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Your search keyword '"Oguma, Keisuke"' showing total 5 results
Start Over Author "Oguma, Keisuke" Journal research in veterinary science
5 results on '"Oguma, Keisuke"'

5. In vitro effect of recombinant human tumor necrosis factor-alpha on canine neutrophil apoptosis.

Author

Oguma K, Sano J, Kano R, Watari T, and Hasegawa A

Subjects
Animals, Cell Survival drug effects, Cycloheximide pharmacology, Dogs, Dose-Response Relationship, Drug, Humans, Protein Synthesis Inhibitors pharmacology, Reactive Oxygen Species metabolism, Recombinant Proteins chemistry, Recombinant Proteins pharmacology, Tumor Necrosis Factor-alpha chemistry, Apoptosis drug effects, Neutrophils cytology, Neutrophils drug effects, Tumor Necrosis Factor-alpha pharmacology
Abstract

Neutrophils (polymorphonuclear leukocytes: PMNs) are essential for the host defense against various infections and are often injurious to the host, causing inflammatory diseases where tumor necrosis factor-alpha (TNF-alpha) is suggested to play an important role. Since an effect of TNF-alpha on canine PMN apoptosis has not been studied, canine PMNs were stimulated with recombinant human (rh)TNF-alpha in the present study to investigate the effect of TNF-alpha on canine PMN apoptosis. PMN apoptosis and function to produce ROS were assessed by flow cytometry. Delayed apoptosis was observed in the PMNs treated with rhTNF-alpha at 100 ng/ml, accompanied by retention of capability to produce ROS. However, PMN apoptosis was accelerated by rhTNF-alpha combined with cycloheximide. Therefore, it is indicated that TNF-alpha is able to activate anti- and pro-apoptotic pathways in PMNs and that the inhibition of PMN apoptosis by TNF-alpha requires protein synthesis in the PMNs.

Published
2006
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