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In vitro effect of recombinant human tumor necrosis factor-alpha on canine neutrophil apoptosis.
- Source :
-
Research in veterinary science [Res Vet Sci] 2006 Apr; Vol. 80 (2), pp. 162-6. Date of Electronic Publication: 2005 Jul 22. - Publication Year :
- 2006
-
Abstract
- Neutrophils (polymorphonuclear leukocytes: PMNs) are essential for the host defense against various infections and are often injurious to the host, causing inflammatory diseases where tumor necrosis factor-alpha (TNF-alpha) is suggested to play an important role. Since an effect of TNF-alpha on canine PMN apoptosis has not been studied, canine PMNs were stimulated with recombinant human (rh)TNF-alpha in the present study to investigate the effect of TNF-alpha on canine PMN apoptosis. PMN apoptosis and function to produce ROS were assessed by flow cytometry. Delayed apoptosis was observed in the PMNs treated with rhTNF-alpha at 100 ng/ml, accompanied by retention of capability to produce ROS. However, PMN apoptosis was accelerated by rhTNF-alpha combined with cycloheximide. Therefore, it is indicated that TNF-alpha is able to activate anti- and pro-apoptotic pathways in PMNs and that the inhibition of PMN apoptosis by TNF-alpha requires protein synthesis in the PMNs.
- Subjects :
- Animals
Cell Survival drug effects
Cycloheximide pharmacology
Dogs
Dose-Response Relationship, Drug
Humans
Protein Synthesis Inhibitors pharmacology
Reactive Oxygen Species metabolism
Recombinant Proteins chemistry
Recombinant Proteins pharmacology
Tumor Necrosis Factor-alpha chemistry
Apoptosis drug effects
Neutrophils cytology
Neutrophils drug effects
Tumor Necrosis Factor-alpha pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0034-5288
- Volume :
- 80
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Research in veterinary science
- Publication Type :
- Academic Journal
- Accession number :
- 16040067
- Full Text :
- https://doi.org/10.1016/j.rvsc.2005.05.012