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Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To investigate the effects of sodium hypochlorite and chlorhexidine as root canal disinfection rinsing solution on oral flora and root canal infection substances. Seventy-six patients who received root canal treatment were divided into observation group and control group according to the random number table method, in which the control group was treated with sodium hypochlorite and the observation group was treated with sodium hypochlorite and chlorhexidine combined. The detection rate of oral flora, bacterial colony count in the root canal, infectious substances in the root canal, therapeutic effect, long-term effective rate and incidence of adverse reactions in two groups were compared and analyzed. After root canal disinfection and irrigation, the bacterial colony count and root canal infection substances in the observation group were significantly lower than those in the control group, with statistical significance (P<0.05). The incidence of total effective rate and adverse reaction in the observation group was 100.00% and 13.16%, respectively, and the incidence of total effective rate and adverse reaction in the control group was 88.00% and 65.79%, respectively (P<0.05). In addition, the effective rate of observation group was higher than control group at 3 months, 6 months and 1 year later. Sodium hypochlorite and chlorhexidine, as root canal disinfection rinsing solutions, can effectively reduce the number of oral flora and infectious substances in the root canal, improve the treatment efficiency and reduce the incidence of adverse reactions. Therefore, they are worthy of widespread clinical use. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the effect of escitalopram combined with psychotherapy on sleep quality, quality of life and serum neurotransmitter levels in patients with anxiety disorder. Methods: 117 patients with anxiety disorder admitted to our hospital from July 2017 to March 2020 were selected, and randomly divided into control group (58 cases) and study group (59 cases). The control group was treated with escitalopram, and the study group was combined with psychotherapy on the basis of the control group. The efficacy, Hamilton Anxiety Scale (HAMA) and Pittsburgh sleep quality index (PSQI) and short form of health survey (SF-36) scores, serum neurotransmitter levels [5-hydroxytryptamine (5-HT), norepinephrine (NE), myeloperoxidase (MPO), neuropeptide (NPY)] and adverse reactions were compared between the two groups. 8 weeks after treatment, the clinical total effective rate of the study group was 88.14% (52/59), which was significantly higher than that of the control group 70.69% (41/58)(P<0.05). Methods: 8 weeks after treatment, the MPO levels of the study group were lower than those of the control group, and the 5-HT, NE and NPY level was higher than that of the control group (P<0.05). 8 weeks after treatment, HAMA and PSQI scores of the study group were lower than those of the control group (P<0.05). 8 weeks after treatment, the scores of mental health, physiological function, physical pain, social function, emotional function, vitality, physiological function and health status of the study group were higher than those of the control group (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion: Escitalopram combined with psychotherapy has good efficacy and safety in the treatment of patients with anxiety disorder. It can effectively improve the anxiety symptoms, sleep quality and quality of life, and effectively regulate the serum neurotransmitter levels. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the effect of steroid coenzyme A desaturase 1(SCD1) overexpression on osteogenic differentiation of bone marrow mesenchymal stem cells(BM-MSCs), and to analyze the changes of whole genome expression profile by gene chip technology. Methods: BM-MSCs were transfected with SCD1 lentivirus. The overexpression and activity of SCD1 in BM-MSCs were detected by RT-PCR and C14 technique. OC and other related osteogenesis indexes were detected by Western blot and alizarin red staining after BM-MSCs were induced and cultured. The effect of overexpression of SCD1 on osteogenic differentiation expression profile of BM-MSCs was further examined by whole gene chip. Results: SCD1 was successfully overexpressed in BM-MSCs,and SCD1 activity in overexpressing group was significantly higher than that in control group. The levels of alkaline phosphatase(APL)and osteocalcin(OC) in the overexpression group were higher than those in the control group at 7 and 14 days after osteogenesis induction(P < 0.05). Alkaline phosphatase staining and alizarin red staining were more in the overexpression group than in the control group at 7 and 14 days after osteogenesis induction. The results of gene expression microarray showed that 2896 differentially expressed genes were detected by overexpression of SCD1 in bone marrow mesenchymal stem cells. Gene pathway analysis showed that the interferon pathway was the most significant pathway(P<0.05). Conclusion: Overexpression of SCD1 can promote the osteogenic differentiation of BM-MSCs, which may affect the osteogenic differentiation function through interferon pathway. This finding may provide important thinking and potential therapeutic strategies for fracture healing, and is worthy of further study. [ABSTRACT FROM AUTHOR]

Objective: To clarify the pathophysiological correlation between alpha-nuclear synaptic protein and Parkinson's disease and its clinical significance. Methods: Chromatography - mass spectrometry (UPLC -ms) detection of wild type mice and endogenous metabolic product of gene mutant mice brain tissue by mzcloud endogenous metabolic substances identified in brain tissue in mice, the corresponding data of principal component analysis (PCA) and cluster analysis, analysis of the relevant differentially expressed metabolites, and construct the circuit diagram and interaction network diagram. Results: (1) the results of metabolism analysis based on LC/MS method showed that the metabolites between the two groups were mainly amino acids and phospholipids, including beta-alanyl-histidine, l-arginine, l-histidine, l-leucine, l-phenylalanine, l-valine, l-aspartic acid, l-alanine, phospholipid choline, etc. (2) build the metabolic pathways of mainly relates to the synthesis and degradation of ketone body, taurine and the metabolism of taurine, alanine, glutamic acid and aspartic acid metabolism, arginine and proline metabolism, histidine metabolism, phenylalanine metabolism, valine, leucine and isoleucine biosynthesis, glycerol phospholipid metabolism, etc., find 18 landmark metabolic components. Conclusion: after alpha nuclear synaptic protein gene mutations, ketone body of synthesis and degradation, taurine and the metabolism of taurine, alanine, glutamic acid and aspartic acid metabolism, arginine and proline metabolism, histidine metabolism, phenylalanine metabolism, valine, leucine and isoleucine biosynthesis and glycerol phospholipid metabolism in the changes of metabolic pathways, involved in beta - propylene ammonia acyl - L - histidine, L - arginine, L - histidine, L leucine, L - phenylalanine, L - valine, L - aspartate, L - alanine, phosphatidyl choline etc. Change the biological signature of metabolites. [ABSTRACT FROM AUTHOR]

Objective: Explored the effect of Stearoyl-CoA desaturase 1 (SCD1) overexpression on the endothelial differentiation function of human bone marrow mesenchymal stem cells (BM-MSCs) and genes expression profile, through the gene chip and intelligent pathway analysis system (IPA). Methods: Used the SCD1 lentivirus which had been successfully built to transfect BM-MSCs. Performed RT-PCR to detect the expression of SCD1 and used ratio of ([ 14C] oleic acid)/ ([14C] oleic and stearic acids) to detected the activity of SCD1. After endothelial induction culture, used RT-PCR to detect the endothelial index including CD31, vWF and CDH5. Gene microarray was performed and used the Ingenuity Pathway Analysis (IPA) system to analyze the differential gene expression profile of the BM-MSCs modulated by SCD1 overexpression. Results: SCD1 was stably overexpressed in BM-MSCs and maintained a highly activity. After 7 days of endothelial induction culture. CD31 and vWF mRXA of overexpression group were higher than that of the control groupp(p< 0.05). and CD31. vWF and CDH5 mRXA of overexpression group were higher than that of the control group after 14 days (p< 0.05). The microarray showed there are total 522 differential expression genes and IPA results show that the genes of NRF2 pathway and the genes of Cell Differentiation Function were significantly differential expression genes (p < 0.05). Conclusion: SCD1 expression can promote the endothelial differentiation of BM-MSCs. probably by reducing the cell oxidative stress and improving the ability of cell proliferation differentiation. This effect of SCD1 may provide the potential strategy for the endothelial function repair and cardiovascular diseases treatment, which is worth further study. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the therapeutic effect of quercetin on rats with cervicitis. Methods: Cervicitis model rats were prepared by vaginal injection of phenol mucilage, the effect of different doses of quercetin(10 μmol·L-1, 100 μmol·L-1) on the number of white blood cells, number and proportion of neutrophil were observed, the rats cervical histopathological and ultrastructural findings（the changes of cell membrane, mitochondria and nuclear structure） were also detected. Results: White blood cells in quercetin group were decreased, counts and percentage of neutrophil cells were significantly reduced, which had statistical significance compared with cervicitis model group（P<0.05）; Histopathological observation results showed that the number of inflammatory cells in quercetin-treated group was lower than that in cervicitis model group, Electron microscopic observation also showed that the ultrastructural damage of cervical cells in quercetin-treated group was reduced, the chromatin condensation in the nucleus was alleviated, and the continuous nuclear membrane structure could be seen gradually. Conclusion: Quercetin can obviously reduce the total number of white blood cells,reduce the cell count and percentage of neutrophil cells, reduce the cervical tissue hyperemia and edema, which have a good therapeutic effect on cervicitis rats. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)