α-核突触蛋白基因突变小鼠脑组织中内源性代谢产物的变化.

Objective: To clarify the pathophysiological correlation between alpha-nuclear synaptic protein and Parkinson's disease and its clinical significance. Methods: Chromatography - mass spectrometry (UPLC -ms) detection of wild type mice and endogenous metabolic product of gene mutant mice brain tissue by mzcloud endogenous metabolic substances identified in brain tissue in mice, the corresponding data of principal component analysis (PCA) and cluster analysis, analysis of the relevant differentially expressed metabolites, and construct the circuit diagram and interaction network diagram. Results: (1) the results of metabolism analysis based on LC/MS method showed that the metabolites between the two groups were mainly amino acids and phospholipids, including beta-alanyl-histidine, l-arginine, l-histidine, l-leucine, l-phenylalanine, l-valine, l-aspartic acid, l-alanine, phospholipid choline, etc. (2) build the metabolic pathways of mainly relates to the synthesis and degradation of ketone body, taurine and the metabolism of taurine, alanine, glutamic acid and aspartic acid metabolism, arginine and proline metabolism, histidine metabolism, phenylalanine metabolism, valine, leucine and isoleucine biosynthesis, glycerol phospholipid metabolism, etc., find 18 landmark metabolic components. Conclusion: after alpha nuclear synaptic protein gene mutations, ketone body of synthesis and degradation, taurine and the metabolism of taurine, alanine, glutamic acid and aspartic acid metabolism, arginine and proline metabolism, histidine metabolism, phenylalanine metabolism, valine, leucine and isoleucine biosynthesis and glycerol phospholipid metabolism in the changes of metabolic pathways, involved in beta - propylene ammonia acyl - L - histidine, L - arginine, L - histidine, L leucine, L - phenylalanine, L - valine, L - aspartate, L - alanine, phosphatidyl choline etc. Change the biological signature of metabolites. [ABSTRACT FROM AUTHOR]