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Objective: To explore the effect of dobutamine combined with amiodarone in the clinical treatment of arrhythmia patients in ICU. Methods: 72 patients with arrhythmias in ICU treated in our hospital from April 2019 to may 2022 were divided into matched group and experimental group by average method, with 36 cases in each group. The matched group was treated with dobutamine, while the experimental group was treated with dobutamine + amiodarone. The cardiac function indexes, BNP and HS CRP levels, the frequency of arrhythmias, average heart rate, clinical efficacy, adverse cardiovascular events and drug safety were compared between the two groups. Results: There was no difference in the levels of LVEF, LVEDd and LVESD between the two groups before treatment (P>0.05). After treatment, the levels of LVEF increased and the levels of LVEDd and LVESD decreased in the two groups. After treatment, the changes of the above indexes in the experimental group were greater than those in the matched group (P<0.05); Before treatment, there was no difference in the levels of BNP and hs CRP between the two groups (P>0.05). After treatment, the levels of BNP and hs CRP in the two groups decreased, and after treatment, the levels of BNP and hs CRP in the experimental group were lower than those in the matched group (P<0.05); Before treatment, there was no difference in the number of arrhythmias and average heart rate between the two groups (P>0.05). After treatment, the number of arrhythmias and average heart rate in the two groups decreased, and after treatment, the number of arrhythmias in the experimental group was less than that in the matched group, and the average heart rate was lower than that in the matched group (P<0.05); The effective rate of clinical treatment in the experimental group was 94.44 %, and that in the matched group was 69.44 %. The effective rate of clinical treatment in the experimental group was higher than that in the matched group (P<0.05); The incidence of adverse cardiovascular events and adverse reactions in the experimental group were lower than those in the matched group (P<0.05). Conclusion: Dobutamine combined with amiodarone has a significant therapeutic effect on improving cardiac function in ICU arrhythmia patients, reducing the number of arrhythmias, stabilizing heart rate, and reducing the level of brain natriuretic peptide and high-sensitivity C-reactive protein, and the patient develops after treatment. There are few adverse cardiovascular events and adverse drug reactions, and it has a satisfactory drug safety. [ABSTRACT FROM AUTHOR]

The study of proteomics is the feature of life science in the post genomic era, an effective means to explore the mystery of life, and related experimental technologies have been widely used in various fields. Traditional proteomic technologies are that include gel electrophoresis, mass spectrometry analysis, protein chip and so on. Due to these technologies existence some deficiencies, i.e. low sensitivity, complicated operation, the disadvantages of poor repeatability, they have not been adapt to the current protein research, which prompted the improvement and innovation of protein group science and technology. Isotope labeled for relative and absolute quantitation (iTRAQ) technology is used for qualitative and quantitative protein commonly, development as a new technology quickly, the technology has the advantages of high sensitivity, accuracy and good reproducibility, which can simultaneously detect multiple samples and the samples can be analysed accurdately and efficiently. In this paper, the advantages and disadvantages of iTRAQ technology are analyzed, the medicine and clinical researches that used the iTRAQ technology in recent years are analyzed, and the application prospects of this technology in the field of medicine are discussed. [ABSTRACT FROM AUTHOR]

The basis of proteomics is protein separation. For natural protein, it could take a series of purification procedures to obtain targeted protein with required purity for research purposes. But the protein may lose its activity for conformational changing caused by the environmental change of solution or external force. In this review, we introduced the frequently used protein purification technologies, such as membrane separation, precipitation, electrophoresis and chromatography, and summarized the existing problems in nowadays protein purification technologies. Moreover, we introduced and prospected the application of the asymmetrical flow field-flow fractionation separation technology in protein separation and purification. [ABSTRACT FROM AUTHOR]

Proteins are the foundation of life, they play an important part in the metabolic process of cell. These proteins which are made of 20 natural amino acids are involved in all the cellular activities and life processes. For example, the polymerase, translation complex and signal receptors which play pivotal roles in the process of DNA replication, RNA translation and signal transduction are all proteins. The activities of proteins are controlled by their spatial configuration and post-translational modifications. With the development of chemistry and biology, researchers created different methods to introduce unnatural amino acids (UAA) into proteins to change the properties of proteins, such as chemical modification and synthesis, in vitro expression system and genetic code expansion. This article reviewed various methods in chemistry and biology for UAA insertion, and discussed their latest applications in research. [ABSTRACT FROM AUTHOR]