114 results on '"Wei, Jia"'
Search Results
2. Tryptophan Predicts the Risk for Future Type 2 Diabetes.
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Tianlu Chen, Xiaojiao Zheng, Xiaojing Ma, Yuqian Bao, Yan Ni, Cheng Hu, Cynthia Rajani, Fengjie Huang, Aihua Zhao, Weiping Jia, and Wei Jia
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Medicine ,Science - Abstract
Recently, 5 amino acids were identified and verified as important metabolites highly associated with type 2 diabetes (T2D) development. This report aims to assess the association of tryptophan with the development of T2D and to evaluate its performance with existing amino acid markers. A total of 213 participants selected from a ten-year longitudinal Shanghai Diabetes Study (SHDS) were examined in two ways: 1) 51 subjects who developed diabetes and 162 individuals who remained metabolically healthy in 10 years; 2) the same 51 future diabetes and 23 strictly matched ones selected from the 162 healthy individuals. Baseline fasting serum tryptophan concentrations were quantitatively measured using ultra-performance liquid chromatography triple quadruple mass spectrometry. First, serum tryptophan level was found significantly higher in future T2D and was positively and independently associated with diabetes onset risk. Patients with higher tryptophan level tended to present higher degree of insulin resistance and secretion, triglyceride and blood pressure. Second, the prediction potential of tryptophan is non-inferior to the 5 existing amino acids. The predictive performance of the combined score improved after taking tryptophan into account. Our findings unveiled the potential of tryptophan as a new marker associated with diabetes risk in Chinese populations. The addition of tryptophan provided complementary value to the existing amino acid predictors.
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- 2016
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3. Meta-analysis of the effect of mesenchymal stem cell transplantation on vascular remodeling after carotid balloon injury in animal models.
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Xinxin Ju, Hong Zou, Kejian Liu, Juncang Duan, Shugang Li, Zheng Zhou, Yan Qi, Jin Zhao, Jianming Hu, Lianghai Wang, Wei Jia, Yutao Wei, Yixun Wang, Wenjie Zhang, Lijuan Pang, and Feng Li
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Medicine ,Science - Abstract
A meta-analysis was conducted to assess the efficacy of mesenchymal stem cell (MSC) transplantation in small animal coronary vessels after balloon injury, to provide data for the design of future pre-clinical experiments and human clinical trials.The search strategy included the PubMed, EMBASE, Chinese Biomedical Literature (CBM), and China National Knowledge Infrastructure (CKNI) databases. The endpoint was the ratio of vascular neointima/media (I/M). Moreover, neointimal area, re-endothelialization, and proliferating cell nuclear antigen (PCNA) expression were analyzed. Pooled analyses were conducted using random effects models. Heterogeneity and publication bias were also explored. All data were analyzed using RevMan 5.2 and Stata 12.0.Fifteen studies were reviewed from 238 retrieved animal studies. Compared with controls, MSC transplantation resulted in greater I/M reduction (pooled difference, 0.39; 95% CI, 0.57-0.21; P < 0.0001), greater neointimal area reduction (pooled difference, 0.16; 95% CI, 0.22-0.10; P < 0.0001), decreased PCNA expression (pooled difference, 17.69; 95% CI, 28.94-6.44; P = 0.002), and enhanced re-endothelialization (pooled difference, 3.37; 95% CI, 1.78-4.95; P < 0.0001). The multivariable meta-regression analysis showed that a higher number of transplanted cells (>106; P = 0.017) and later time point of I/M measurement (P = 0.022) were significantly associated with I/M reduction. Subgroup analysis demonstrated a trend for a greater reduction in the ratio of I/M with late MSC transplantation (>1 day), MSCs transplanted through intravenous injection, and atherosclerotic vessels.The meta-analysis results demonstrate that MSC transplantation might improve injured vascular remodeling. In addition to greater efficacy with a greater number of transplanted MSCs (>106), the long-term effect of MSC transplantation appears to be more significant. The findings of this meta-analysis may help to design future, effective MSC trials.
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- 2015
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4. The Features of Genetic Prion Diseases Based on Chinese Surveillance Program.
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Qi Shi, Wei Zhou, Cao Chen, Bao-Yun Zhang, Kang Xiao, Xiu-Chun Zhang, Xiao-Jing Shen, Qing Li, Li-Quan Deng, Jian-Hua Dong, Wen-Qing Lin, Pu Huang, Wei-Jia Jiang, Jie Lv, Jun Han, and Xiao-Ping Dong
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Medicine ,Science - Abstract
To identify the features of Chinese genetic prion diseases.Suspected Creutzfeldt-Jakob disease (CJD) cases that were reported under CJD surveillance were diagnosed and subtyped using the diagnostic criteria issued by the WHO. The general information concerning the patient, their clinical, MRI and EEG data, and the results of CSF 14-3-3 and PRNP sequencing were carefully collected from the database of the national CJD surveillance program and analyzed using the SPSS 11.5 statistical software program.Since 2006, 69 patients were diagnosed with genetic prion diseases and as having 15 different mutations. The median age of the 69 patients at disease onset was 53.5 years, varying from 19 to 80 years. The majority of patients displaying clinical symptoms were in the 50-59 years of age. FFI, T188K gCJD and E200K were the three most common subtypes. The disease appeared in the family histories of 43.48% of the patients. The clinical manifestations varied considerably among the various diseases. Patients who carried mutations in the N-terminus displayed a younger age of onset, were CSF 14-3-3 negative, had a family history of the condition, and experienced a longer duration of the condition. The clinical courses of T188K were significantly shorter than those of FFI and E200K gCJD, while the symptoms in the FFI group appeared at a younger age and for a longer duration. Moreover, the time intervals between the initial neurologist visit to the final diagnosis were similar among patients with FFI, T188K gCJD, E200K gCJD and other diseases.The features of Chinese genetic prion diseases are different from those seen in Europe and other Asian countries.
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- 2015
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5. Lysophosphatidic Acid Mediates Activating Transcription Factor 3 Expression Which Is a Target for Post-Transcriptional Silencing by miR-30c-2-3p.
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Ha T Nguyen, Wei Jia, Aaron M Beedle, Eileen J Kennedy, and Mandi M Murph
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Medicine ,Science - Abstract
Although microRNAs (miRNAs) are small, non-protein-coding entities, they have important roles in post-transcriptional regulation of most of the human genome. These small entities generate fine-tuning adjustments in the expression of mRNA, which can mildly or massively affect the abundance of proteins. Previously, we found that the expression of miR-30c-2-3p is induced by lysophosphatidic acid and has an important role in the regulation of cell proliferation in ovarian cancer cells. The goal here is to confirm that ATF3 mRNA is a target of miR-30c-2-3p silencing, thereby further establishing the functional role of miR-30c-2-3p. Using a combination of bioinformatics, qRT-PCR, immunoblotting and luciferase assays, we uncovered a regulatory pathway between miR-30c-2-3p and the expression of the transcription factor, ATF3. Lysophosphatidic acids triggers the expression of both miR-30c-2-3p and ATF3, which peak at 1 h and are absent 8 h post stimulation in SKOV-3 and OVCAR-3 serous ovarian cancer cells. The 3´-untranslated region (3´-UTR) of ATF3 was a predicted, putative target for miR-30c-2-3p, which we confirmed as a bona-fide interaction using a luciferase reporter assay. Specific mutations introduced into the predicted site of interaction between miR-30c-2-3p and the 3´-UTR of ATF3 alleviated the suppression of the luciferase signal. Furthermore, the presence of anti-miR-30c-2-3p enhanced ATF3 mRNA and protein after lysophosphatidic acid stimulation. Thus, the data suggest that after the expression of ATF3 and miR-30c-2-3p are elicited by lysophosphatidic acid, subsequently miR-30c-2-3p negatively regulates the expression of ATF3 through post-transcriptional silencing, which prevents further ATF3-related outcomes as a consequence of lysophosphatidic acid signaling.
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- 2015
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6. The prognostic value of altered eIF3a and its association with p27 in non-small cell lung cancers.
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Jie Shen, Ji-Ye Yin, Xiang-Ping Li, Zhao-Qian Liu, Ying Wang, Juan Chen, Jian Qu, Xiao-Jing Xu, Howard Lewis McLeod, Yi-Jing He, Kun Xia, Yuan-Wei Jia, and Hong-Hao Zhou
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Medicine ,Science - Abstract
BACKGROUND: Over-expressed eukaryotic initiation factor 3a (eIF3a) in non-small cell lung cancer (NSCLC) contributed to cisplatin sensitivity. However, the role of eIF3a in oncogenesis was still controversial. This study was designed to investigate the prognostic impact of eIF3a and p27 in radically resected NSCLC patients. METHODS: The expression levels of subcellular eIF3a and p27 were evaluated immunohistochemically in 537 radically resected NSCLC samples, and another cohort of 210 stage II NSCLC patients. Disease specific survival (DSS) and disease free survival (DFS) were analyzed by Kaplan-Meier method and Cox regression model. RESULTS: The subcellular expression of eIF3a was strongly correlated with status of p27 (Spearman rank coefficient correlation for cytoplasmic eIF3a and p27=0.653, for nuclear staining=0.716). Moreover, survival analysis revealed favorable prognostic impact of nuclear eIF3a, p27, and the combination high nuclear staining on NSCLC (Hazards Ratio=0.360, 95%CI=0.109-0.782, P=0.028). In addition, interaction research between biomarkers and chemotherapy status disclosed cisplatin-based regimen trend to prolong DSS of stage II NSCLC patients with high eIF3a-C (P=0.036)and low p27-N (P=0.031). CONCLUSIONS: Our findings suggested altered eIF3a expression closely correlated with p27 status, and the association was of prognostic value for resected NSCLC. Altered expression of eIF3a and p27 predicted prognosis of NSCLC independently.
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- 2014
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7. Metabotropic glutamate receptor 3 is associated with heroin dependence but not depression or schizophrenia in a Chinese population.
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Wei Jia, Rui Zhang, Bin Wu, Zun-xiao Dai, Yong-sheng Zhu, Ping-ping Li, and Feng Zhu
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Medicine ,Science - Abstract
Metabotropic glutamate receptor subtype 3 (mGluR3, encoded by GRM3) plays important roles in the pathophysiology of schizophrenia, depression, and drug dependence. GRM3 polymorphisms were reported to be associated with prefrontal activity, cognitive shifting, and memory capability in healthy subjects, as well as susceptibility to schizophrenia and depression. The goal of this study was to replicate the association of GRM3 with schizophrenia and depression and to explore GRM3's potential association with heroin dependence (HD) in a Chinese population. Seventeen SNPs throughout the GRM3 gene were genotyped using MALDI-TOF within the MassARRAY system, and the allele and genotype distributions were compared between 619 healthy controls and 433 patients with schizophrenia, 409 patients with major depression, and 584 unrelated addicts. We found that GRM3 polymorphisms modulate the susceptibility to HD but do not significantly influence the risk for schizophrenia or depression. An increased risk of HD was significantly associated with the minor alleles of two GRM3 SNPs, including the T allele of rs274618 (Odds ratio (OR) = 1.631, 95% confidence interval (95%CI): 1.317-2.005), the T allele of rs274622 (OR = 1.652, 95% CI: 1.336-2.036), compared with the major alleles. The addicts carrying the minor allele of rs274618 or rs274622 had a shortened duration for transition from first use to dependence (DTFUD) in comparison to homozygote for major allele (P
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- 2014
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8. The strain at bone-implant interface determines the effect of spinopelvic reconstruction following total sacrectomy: a strain gauge analysis in various spinopelvic constructs.
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Yan Yu, Rui Zhu, Zhi-Li Zeng, Yong-Wei Jia, Zhou-Rui Wu, Yi-Long Ren, Bo Chen, Zu-Quan Ding, and Li-Ming Cheng
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Medicine ,Science - Abstract
PURPOSE: There is still some controversy regarding the optimal biomechanical concept for spinopelvic stabilization following total sacrectomy for malignancy. Strains at specific anatomical sites at pelvis/sacrum and implants interfaces have been poorly investigated. Herein, we compared and analyzed the strains applied at key points at the bone-implant interface in four different spinopelvic constructs following total sacrectomy; consequently, we defined a balanced architecture for spinopelvic fusion in that situation. METHODS: Six human cadaveric specimens, from second lumbar vertebra to proximal femur, were used to compare the partial strains at specific sites in a total sacrectomy model. Test constructs included: (1) intact pelvis (control), (2) sacral-rod reconstruction (SRR), (3) bilateral fibular flap reconstruction (BFFR), (4) four-rods reconstruction (FRR), and (5) improved compound reconstruction (ICR). Strains were measured by bonded strain gauges onto the surface of three specific sites (pubic rami, arcuate lines, and posterior spinal rods) under a 500 N axial load. RESULTS: ICR caused lower strains at specific sites and, moreover, on stress distribution and symmetry, compared to the other three constructs. Strains at pubic rami and arcuate lines following BFFR were lower than those following SRR, but higher at the posterior spinal rod construct. The different modes of strain distribution reflected different patient's parameter-related conditions. FRR model showed the highest strains at all sites because of the lack of an anterior bracing frame. CONCLUSIONS: The findings of this investigation suggest that both anterior bracing frame and the four-rods load dispersion provide significant load sharing. Additionally, these two constructs decrease the peak strains at bone-implant interface, thus determining the theoretical surgical technique to achieve optimal stress dispersion and balance for spinopelvic reconstruction in early postoperative period following total sacrectomy.
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- 2014
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9. MicroRNA-92a as a potential biomarker in diagnosis of colorectal cancer: a systematic review and meta-analysis.
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Xin Yang, Zongyue Zeng, Yixuan Hou, Taixian Yuan, Chao Gao, Wei Jia, Xiaoyan Yi, and Manran Liu
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Medicine ,Science - Abstract
INTRODUCTION: Previous studies demonstrated that MicroRNA-92a (miR-92a) was significantly differential expressed between colorectal cancer (CRC) patients and control cohorts, which provide timely relevant evidence for miR-92a as a novel promising biomarker in the colorectal cancer patients. This meta-analysis aimed to evaluate potential diagnostic value of plasma miR-92a. METHODS: Relevant literatures were collected in PubMed, Embase, Chinese Biomedical Literature Database (CBM), Chinese National Knowledge Infrastructure (CNKI) and Technology of Chongqing (VIP), and Wan Fang Data. Sensitivity, specificity and diagnostic odds ratio (DOR) for miR-92a in the diagnosis of CRC were pooled using random effects models. Summary receiver operating characteristic (SROC) curve analysis and the area under the curve (AUC) were used to estimate the overall test performance. RESULTS: This Meta-analysis included six studies with a total of 521 CRC patients and 379 healthy controls. For miR-92a, the pooled sensitivity, specificity and DOR to predict CRC patients were 76% (95% confidence interval [CI]: 72%-79%), 64% (95% confidence interval [CI]: 59%-69%) and 8.05 (95% CI: 3.50-18.56), respectively. In addition, the AUC of miR-92a in diagnosis CRC is 0.7720. CONCLUSIONS: MicroRNA-92a might be a novel potential biomarker in the diagnosis of colorectal cancer, and more studies are needed to highlight the theoretical strengths.
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- 2014
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10. Long-term effects of methadone maintenance treatment with different psychosocial intervention models.
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Lirong Wang, Xiaoli Wei, Xueliang Wang, Jinsong Li, Hengxin Li, and Wei Jia
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Medicine ,Science - Abstract
This study evaluated the long-term effects of different psychosocial intervention models in methadone maintenance treatment (MMT) in Xi'an China. Patients from five MMT clinics were divided into three groups receiving MMT only, MMT with counseling psychology (CP) or MMT with contingency management (CM). A five-year follow-up was carried out with daily records of medication, monthly random urine morphine tests, and tests for anti-HIV and anti-HCV every six months. Drug use behavior was recorded six months after initial recruitment using a survey. Adjusted RRs and their 95% confidence intervals (CIs) were estimated using an unconditional logistic regression model or a Cox proportional hazard model. A total of 2662 patients were recruited with 797 in MMT, 985 in MMT with CP, and 880 in MMT with CM. Following six months of treatment, the injection rates of MMT with CP and MMT with CM groups were significantly lower than that of MMT (5.1% and 6.9% vs. 16.3%, x² = 47.093 and 29.908, respectively; P
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- 2014
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11. TGF-β1/Smad signaling pathway regulates epithelial-to-mesenchymal transition in esophageal squamous cell carcinoma: in vitro and clinical analyses of cell lines and nomadic Kazakh patients from northwest Xinjiang, China.
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Lijuan Pang, Qiuxiang Li, Cuilei Wei, Hong Zou, Shugang Li, Weiwei Cao, Jianwei He, Yang Zhou, Xinxin Ju, Jiaojiao Lan, Yutao Wei, Chengyan Wang, Wei Zhao, Jianming Hu, Wei Jia, Yan Qi, Fudong Liu, Jinfang Jiang, Li Li, Jin Zhao, Weihua Liang, Jianxin Xie, and Feng Li
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Medicine ,Science - Abstract
Invasion and metastasis are the major causes of death in patients with esophageal squamous cell carcinoma (ESCC). Epithelial-mesenchymal transition (EMT) is a critical step in tumor progression and transforming growth factor-β1 (TGF-β1) signaling has been shown to play an important role in EMT. In this study, we investigated how TGF-β1 signaling pathways contributed to EMT in three ESCC cell lines as well as 100 patients of nomadic ethnic Kazakhs residing in northwest Xinjiang Province of China. In vitro analyses included Western blotting to detect the expression of TGF-β1/Smad and EMT-associated proteins in Eca109, EC9706 and KYSE150 cell lines following stimulation with recombinant TGF-β1 and SB431542, a potent inhibitor of ALK5 that also inhibits TGF-β type II receptor. TGF-β-activated Smad2/3 signaling in EMT was significantly upregulated as indicated by mesenchymal markers of N-cadherin and Vimentin, and in the meantime, epithelial marker, E-cadherin, was markedly downregulated. In contrast, SB431542 addition downregulated the expression of N-cadherin and Vimentin, but upregulated the expression of E-cadherin. Moreover, the TGF-β1-induced EMT promoted invasion capability of Eca109 cells. Tumor cells undergoing EMT acquire fibroblastoid-like phenotype. Expressed levels of TGF-β1/Smad signaling molecules and EMT-associated proteins were examined using immunohistochemical analyses in 100 ESCC tissues of Kazakh patients and 58 matched noncancerous adjacent tissues. The results showed that ESCC tissues exhibited upregulated expression of TGF-β1/Smad. We also analyzed the relationship between the above proteins and the patients' clinicopathological characteristics. The TGF-β1/Smad signaling pathway in human Eca109 ESCC cells may carry similar features as in Kazakh ESCC patients, suggesting that TGF-β1/Smad signaling pathway may be involved in the regulation of EMT in ethnic Kazakh patients with ESCC from Xinjiang, China.
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- 2014
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12. Statin use is associated with reduced risk of haematological malignancies: evidence from a meta-analysis.
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Xiao Yi, Wei Jia, Yin Jin, and Shang Zhen
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Medicine ,Science - Abstract
BACKGROUND: Several observational studies have shown that statin use may modify the risk of haematological malignancies. To quantify the association between statin use and risk for haematological malignancies, we performed a detailed meta-analysis of published studies regarding this subject. METHODS: We conducted a systematic search of multiple databases including PubMed, Embase, and Cochrane Library Central database up to July 2013. Fixed-effect and random-effect models were used to estimate summary relative risks (RR) and the corresponding 95% confidence intervals (CIs). Potential sources of heterogeneity were detected by meta-regression. Subgroup analyses and sensitivity analysis were also performed. RESULTS: A total of 20 eligible studies (ten case-control studies, four cohort studies, and six RCTs) reporting 1,139,584 subjects and 15,297 haematological malignancies cases were included. Meta-analysis showed that statin use was associated with a statistically significant 19% reduction in haematological malignancies incidence (RR = 0.81, 95% CI [0.70, 0.92]). During subgroup analyses, statin use was associated with a significantly reduced risk of haematological malignancies among observational studies (RR = 0.79, 95% CI [0.67, 0.93]), but not among RCTs (RR = 0.92, 95% CI [0.77, 1.09]). CONCLUSIONS: Based on this comprehensive meta-analysis, statin use may have chemopreventive effects against haematological malignancies. More studies, especially definitive, randomized chemoprevention trials are needed to confirm this association.
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- 2014
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13. High fat diet feeding exaggerates perfluorooctanoic acid-induced liver injury in mice via modulating multiple metabolic pathways.
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Xiaobing Tan, Guoxiang Xie, Xiuhua Sun, Qiong Li, Wei Zhong, Peter Qiao, Xinguo Sun, Wei Jia, and Zhanxiang Zhou
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Medicine ,Science - Abstract
High fat diet (HFD) is closely linked to a variety of health issues including fatty liver. Exposure to perfluorooctanoic acid (PFOA), a synthetic perfluorinated carboxylic acid, also causes liver injury. The present study investigated the possible interactions between high fat diet and PFOA in induction of liver injury. Mice were pair-fed a high-fat diet (HFD) or low fat control with or without PFOA administration at 5 mg/kg/day for 3 weeks. Exposure to PFOA alone caused elevated plasma alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels and increased liver weight along with reduced body weight and adipose tissue mass. HFD alone did not cause liver damage, but exaggerated PFOA-induced hepatotoxicity as indicated by higher plasma ALT and AST levels, and more severe pathological changes including hepatocyte hypertrophy, lipid droplet accumulation and necrosis as well as inflammatory cell infiltration. These additive effects of HFD on PFOA-induced hepatotoxicity correlated with metabolic disturbance in liver and blood as well as up-regulation of hepatic proinflammatory cytokine genes. Metabolomic analysis demonstrated that both serum and hepatic metabolite profiles of PFOA, HFD, or HFD-PFOA group were clearly differentiated from that of controls. PFOA affected more hepatic metabolites than HFD, but HFD showed positive interaction with PFOA on fatty acid metabolites including long chain fatty acids and acylcarnitines. Taken together, dietary high fat potentiates PFOA-induced hepatic lipid accumulation, inflammation and necrotic cell death by disturbing hepatic metabolism and inducing inflammation. This study demonstrated, for the first time, that HFD increases the risk of PFOA in induction of hepatotoxicity.
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- 2013
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14. Dopamine D1 receptor gene variation modulates opioid dependence risk by affecting transition to addiction.
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Feng Zhu, Chun-xia Yan, Yi-chong Wen, Jiayin Wang, Jinbo Bi, Ya-ling Zhao, Lai Wei, Cheng-ge Gao, Wei Jia, and Sheng-bin Li
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Medicine ,Science - Abstract
Dopamine D1 receptor (DRD1) modulates opioid reinforcement, reward, and opioid-induced neuroadaptation. We propose that DRD1 polymorphism affects susceptibility to opioid dependence (OD), the efficiency of transition to OD, and opioid-induced pleasure response. We analyzed potential association between seven DRD1 polymorphisms with the following traits: duration of transition from the first use to dependence (DTFUD), subjective pleasure responses to opioid on first use and post-dependence use, and OD risk in 425 Chinese with OD and 514 healthy controls. DTFUD and level of pleasure responses were examined using a semi-structured interview. The DTFUD of opioid addicts ranged from 5 days to 11 years. Most addicts (64.0%) reported non-comfortable response upon first opioid use, while after dependence, most addicts (53.0%) felt strong opioid-induced pleasure. Survival analysis revealed a correlation of prolonged DTFUD with the minor allele-carrying genotypes of DRD1 rs4532 (hazard ratios (HR) = 0.694; p = 0.001) and rs686 (HR = 0.681, p = 0.0003). Binary logistic regression indicated that rs10063995 GT genotype (vs. GG+TT, OR = 0.261) could predict decreased pleasure response to first-time use and the minor alleles of rs686 (OR = 0.535) and rs4532 (OR = 0.537) could predict decreased post-dependence pleasure. Moreover, rs686 minor allele was associated with a decreased risk for rapid transition from initial use to dependence (DTFUD≤30 days; OR = 0.603) or post-dependence euphoria (OR = 0.603) relative to major allele. In conclusion, DRD1 rs686 minor allele decreases the OD risk by prolonging the transition to dependence and attenuating opioid-induced pleasure in Chinese.
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- 2013
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15. Correction: Differential Regulation of MAPK Phosphorylation in the Dorsal Hippocampus in Response to Prolonged Morphine Withdrawal-Induced Depressive-Like Symptoms in Mice.
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Wei Jia, Rui Liu, Jianguo Shi, Bin Wu, Wei Dang, Ying Du, Qiong Zhou, Jianhua Wang, and Rui Zhang
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Medicine ,Science - Published
- 2013
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16. Differential Regulation of MAPK Phosphorylation in the Dorsal Hippocampus in Response to Prolonged Morphine Withdrawal-Induced Depressive-Like Symptoms in Mice.
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Wei Jia, Rui Liu, Jianguo Shi, Bin Wu, Wei Dang, Ying Du, Qiong Zhou, Jianhua Wang, and Rui Zhang
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Medicine ,Science - Abstract
Depression is one of the most frequent neuropsychiatric comorbidities associated with opiate addiction. Mitogen activated protein kinase (MAPK) and MAPK phosphatase (MKP) are involved in drug addiction and depression. However, the potential role of MAPK and MKP in depression caused by morphine withdrawal remains unclear. We utilized a mouse model of repeated morphine administration to examine the molecular mechanisms that contribute to prolonged withdrawal induced depressive-like behaviors. Depressive-like behaviors were significant at 1 week after withdrawal and worsened over time. Phospho-ERK (extracellular signal-regulated protein kinase) was decreased and MKP-1 was elevated in the hippocampus, and JNK (c-Jun N-terminal protein kinase), p38 (p38 protein kinase) and MKP-3 were unaffected. A pharmacological blockade of MKP-1 by intra-hippocampal sanguinarine (SA) infusion prevented the development of depressive-like behaviors and resulted in relatively normal levels of MKP-1 and phospho-ERK after withdrawal. Our findings support the association between hippocampal MAPK phosphorylation and prolonged morphine withdrawal-induced depression, and emphasize the MKP-1 as an negative regulator of the ERK phosphorylation that contributes to depression.
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- 2013
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17. The metabolic responses to aerial diffusion of essential oils.
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Yani Wu, Yinan Zhang, Guoxiang Xie, Aihua Zhao, Xiaolan Pan, Tianlu Chen, Yixue Hu, Yumin Liu, Yu Cheng, Yi Chi, Lei Yao, and Wei Jia
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Medicine ,Science - Abstract
Anxiety disorders are the most prevalent psychiatric disorders and affect a great number of people worldwide. Essential oils, take effects through inhalation or topical application, are believed to enhance physical, emotional, and spiritual well-being. Although clinical studies suggest that the use of essential oils may have therapeutic potential, evidence for the efficacy of essential oils in treating medical conditions remains poor, with a particular lack of studies employing rigorous analytical methods that capture its identifiable impact on human biology. Here, we report a comprehensive gas chromatography time-of-flight mass spectrometry (GC-TOFMS) based metabonomics study that reveals the aromas-induced metabolic changes and the anxiolytic effect of aromas in elevated plus maze (EPM) induced anxiety model rats. The significant alteration of metabolites in the EPM group was attenuated by aromas treatment, concurrent with the behavioral improvement with significantly increased open arms time and open arms entries. Brain tissue and urinary metabonomic analysis identified a number of altered metabolites in response to aromas intervention. These metabolic changes included the increased carbohydrates and lowered levels of neurotransmitters (tryptophan, serine, glycine, aspartate, tyrosine, cysteine, phenylalanine, hypotaurine, histidine, and asparagine), amino acids, and fatty acids in the brain. Elevated aspartate, carbohydrates (sucrose, maltose, fructose, and glucose), nucleosides and organic acids such as lactate and pyruvate were also observed in the urine. The EPM induced metabolic differences observed in urine or brain tissue was significantly reduced after 10 days of aroma inhalation, as noted with the loss of statistical significance on many of the metabolites in the aroma-EPM group. This study demonstrates, for the first time, that the metabonomics approach can capture the subtle metabolic changes resulting from exposure to essential oils and provide the basis for pinpointing affected pathways in anxiety-related behavior, which will lead to an improved mechanistic understanding of anxiolytic effect of essential oils.
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- 2012
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18. Increased p66Shc in the inner ear of D-galactose-induced aging mice with accumulation of mitochondrial DNA 3873-bp deletion: p66Shc and mtDNA damage in the inner ear during aging.
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Lisa Wu, Yu Sun, Yu-Juan Hu, Yang Yang, Ling-Li Yao, Xing-Xing Zhou, Hao Wang, Rui Zhang, Xiang Huang, and Wei-Jia Kong
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Medicine ,Science - Abstract
Aging has been associated with mitochondrial DNA damage. P66Shc is an age-related adaptor protein that has a substantial impact on mitochondrial metabolism through regulation of the cellular response to oxidative stress. Our study aimed to establish a D-galactose (D-gal)-induced inner ear aging mouse model and to investigate the potential role of p66Shc and its serine 36-phosphorylated form in the inner ear during aging by using this model. Real-time PCR was performed to detect the mtDNA 3873-bp deletion and the level of p66Shc mRNA in the cochlear lateral wall. Western blot analysis was performed to analyze the total and mitochondrial protein levels of p66Shc and the level of Ser36-P-p66Shc in the cochlear lateral wall. Immunofluoresence was performed to detect the location of the Ser36-P-p66Shc expression in the cochlear lateral wall. The results showed that the accumulation of the mtDNA 3873-bp deletion, total and mitochondrial protein levels of p66Shc and level of Ser36-P-p66Shc were significantly increased in the cochlear lateral wall of the D-gal-treated group when compared to the control group and that Ser36-P-p66Shc was mainly localized in the cytoplasm of the cells in the stria vascularis. During aging, the oxidative stress-related increase of p66Shc and Ser36-P-p66Shc might be associated with the accumulation of the mtDNA 3873-bp deletion in the inner ear.
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- 2012
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19. Seminal plasma metabolomics approach for the diagnosis of unexplained male infertility
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Minjian Chen, Qiuqin Tang, Wei Wu, Xinru Wang, Wei Jia, Yankai Xia, and Shanlei Qiao
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0301 basic medicine ,Male ,Physiology ,lcsh:Medicine ,Biochemistry ,Mass Spectrometry ,Male infertility ,Analytical Chemistry ,0302 clinical medicine ,Spectrum Analysis Techniques ,Animal Cells ,Metabolites ,Medicine and Health Sciences ,Medicine ,lcsh:Science ,030219 obstetrics & reproductive medicine ,Multidisciplinary ,Chromatographic Techniques ,Body Fluids ,Chemistry ,Physical Sciences ,Metabolome ,Population study ,Metabolic Pathways ,Anatomy ,Cellular Types ,Metabolic Networks and Pathways ,Research Article ,Infertility ,Adult ,Urology ,Semen ,Plasma biomarkers ,Research and Analysis Methods ,Gas Chromatography-Mass Spectrometry ,Andrology ,03 medical and health sciences ,Metabolomics ,Humans ,Male Infertility ,Infertility, Male ,business.industry ,lcsh:R ,Biology and Life Sciences ,Cell Biology ,Pathway enrichment ,medicine.disease ,Sperm ,030104 developmental biology ,Metabolism ,Germ Cells ,lcsh:Q ,business ,Biomarkers - Abstract
We used a gas chromatography-mass spectrometry (GC-MS) based metabolomics approach to obtain the metabolic profiling of unexplained male infertility (UMI), and identified seminal plasma biomarkers associated with UMI by a two-stage population study. A robust OPLS-DA model based on these identified metabolites was able to distinguish 82% of the UMI patients from health controls with a specificity of 92%. In this model, 44 metabolites were found differentially expressed in UMI subjects compared with health controls. By pathway enrichment analysis, we identified several major changed metabolic pathways related to UMI. Our findings provide new perspective for the diagnosis of UMI.
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- 2017
20. Consideration of future consequences (CFC) serves as a buffer against aggression related to psychopathy
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Zhao, Yongping, primary, Wei, Jia, additional, Chen, Yuanshu, additional, and Xia, Lingxiang, additional
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- 2018
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21. Reconstitution of Protein Translation of Mycobacterium Reveals Functional Conservation and Divergence with the Gram-Negative Bacterium Escherichia coli
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Aashish Srivastava, Sheng Cui, Meng Zhang, Haiying Liu, Shaorong Chong, Wei-Jia Zhang, Haruichi Asahara, and Qi Jin
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0301 basic medicine ,lcsh:Medicine ,Gene Expression ,Centrifugation ,Protein Synthesis ,medicine.disease_cause ,Ribosome ,Biochemistry ,Antibiotics ,Protein biosynthesis ,Medicine and Health Sciences ,lcsh:Science ,Multidisciplinary ,biology ,Antimicrobials ,Mycobacterium smegmatis ,Drugs ,Chemical Synthesis ,Translation (biology) ,Actinobacteria ,Nucleic acids ,Separation Processes ,Protein Transport ,Transfer RNA ,Cellular Structures and Organelles ,Research Article ,Biosynthetic Techniques ,030106 microbiology ,Research and Analysis Methods ,Microbiology ,Mycobacterium ,Mycobacterium tuberculosis ,Amino Acyl-tRNA Synthetases ,03 medical and health sciences ,Microbial Control ,Elongation Factors ,medicine ,Genetics ,Escherichia coli ,Gene Regulation ,Non-coding RNA ,Pharmacology ,Bacteria ,lcsh:R ,Organisms ,Biology and Life Sciences ,Proteins ,Cell Biology ,biology.organism_classification ,Regulatory Proteins ,030104 developmental biology ,RNA ,lcsh:Q ,Protein Translation ,Ribosomes ,Mycobacterium Tuberculosis - Abstract
Protein translation is essential for all bacteria pathogens. It has also been a major focus of structural and functional studies and an important target of antibiotics. Here we report our attempts to biochemically reconstitute mycobacterial protein translation in vitro from purified components. This mycobacterial translation system consists of individually purified recombinant translation factors from Mycobacterium tuberculosis (M. tuberculosis), purified tRNAs and ribosomes from Mycobacterium smegmatis (M. smegmatis), and an aminoacyl-tRNA synthetase (AARS) mixture from the cell-extract of M. smegmatis. We demonstrate that such mycobacterial translation system was efficient in in vitro protein synthesis, and enabled functional comparisons of translational components between the gram-positive Mycobacterium and the gram-negative E. coli. Although mycobacterial translation factors and ribosomes were highly compatible with their E. coli counterparts, M. smegmatis tRNAs were not properly charged by the E. coli AARSs to allow efficient translation of a reporter. In contrast, both E. coli and M. smegmatis tRNAs exhibited similar activity with the semi-purified M. smegmatis AARSs mixture for in vitro translation. We further demonstrated the use of both mycobacterial and E. coli translation systems as comparative in vitro assays for small-molecule antibiotics that target protein translation. While mycobacterial and E. coli translation were both inhibited at the same IC50 by the antibiotic spectinomycin, mycobacterial translation was preferentially inhibited by the antibiotic tetracycline, suggesting that there may be structural differences at the antibiotic binding sites between the ribosomes of Mycobacterium and E. coli. Our results illustrate an alternative approach for antibiotic discovery and functional studies of protein translation in mycobacteria and possibly other bacterial pathogens.
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- 2016
22. Tryptophan Predicts the Risk for Future Type 2 Diabetes
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Weiping Jia, Cynthia Rajani, Yuqian Bao, Tianlu Chen, Fengjie Huang, Cheng Hu, Wei Jia, Aihua Zhao, Xiaojing Ma, Yan Ni, and Xiaojiao Zheng
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0301 basic medicine ,Blood Glucose ,Male ,Physiology ,medicine.medical_treatment ,lcsh:Medicine ,Type 2 diabetes ,Biochemistry ,chemistry.chemical_compound ,Aromatic Amino Acids ,Endocrinology ,Glucose Metabolism ,Risk Factors ,Insulin Secretion ,Medicine and Health Sciences ,Insulin ,Longitudinal Studies ,Amino Acids ,lcsh:Science ,Multidisciplinary ,Organic Compounds ,Tryptophan ,Valine ,Fasting ,Middle Aged ,Prognosis ,Chemistry ,Physical Sciences ,Carbohydrate Metabolism ,Female ,Research Article ,Adult ,medicine.medical_specialty ,China ,Diabetes risk ,Endocrine Disorders ,Phenylalanine ,Biology ,03 medical and health sciences ,Insulin resistance ,Leucine ,Predictive Value of Tests ,Internal medicine ,Diabetes mellitus ,Hydroxyl Amino Acids ,medicine ,Diabetes Mellitus ,Humans ,Isoleucine ,Aged ,Diabetic Endocrinology ,Triglyceride ,Endocrine Physiology ,lcsh:R ,Organic Chemistry ,Chemical Compounds ,Biology and Life Sciences ,Proteins ,medicine.disease ,Hormones ,030104 developmental biology ,Metabolism ,chemistry ,Diabetes Mellitus, Type 2 ,Aliphatic Amino Acids ,Case-Control Studies ,Metabolic Disorders ,Tyrosine ,lcsh:Q ,Insulin Resistance ,Biomarkers - Abstract
Recently, 5 amino acids were identified and verified as important metabolites highly associated with type 2 diabetes (T2D) development. This report aims to assess the association of tryptophan with the development of T2D and to evaluate its performance with existing amino acid markers. A total of 213 participants selected from a ten-year longitudinal Shanghai Diabetes Study (SHDS) were examined in two ways: 1) 51 subjects who developed diabetes and 162 individuals who remained metabolically healthy in 10 years; 2) the same 51 future diabetes and 23 strictly matched ones selected from the 162 healthy individuals. Baseline fasting serum tryptophan concentrations were quantitatively measured using ultra-performance liquid chromatography triple quadruple mass spectrometry. First, serum tryptophan level was found significantly higher in future T2D and was positively and independently associated with diabetes onset risk. Patients with higher tryptophan level tended to present higher degree of insulin resistance and secretion, triglyceride and blood pressure. Second, the prediction potential of tryptophan is non-inferior to the 5 existing amino acids. The predictive performance of the combined score improved after taking tryptophan into account. Our findings unveiled the potential of tryptophan as a new marker associated with diabetes risk in Chinese populations. The addition of tryptophan provided complementary value to the existing amino acid predictors.
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- 2016
23. Repositioning Bazedoxifene as a novel IL-6/GP130 signaling antagonist for human rhabdomyosarcoma therapy
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Xiao, Hui, primary, Bid, Hemant Kumar, additional, Chen, Xiang, additional, Wu, Xiaojuan, additional, Wei, Jia, additional, Bian, Yang, additional, Zhao, Chengguang, additional, Li, Huameng, additional, Li, Chenglong, additional, and Lin, Jiayuh, additional
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- 2017
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24. Superior success rate of intracavitary electrocardiogram guidance for peripherally inserted central catheter placement in patients with cancer: A randomized open-label controlled multicenter study
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Yuan, Ling, primary, Li, Rongmei, additional, Meng, Aifeng, additional, Feng, Yuling, additional, Wu, Xiancui, additional, Yang, Yiqun, additional, Chen, Ping, additional, Qiu, Zhenzhu, additional, Qi, Jing, additional, Chen, Chuanying, additional, Wei, Jia, additional, Qin, Minyi, additional, Kong, Weiwei, additional, Chen, Xiangyu, additional, and Xu, Wei, additional
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- 2017
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25. Meta-analysis of the effect of mesenchymal stem cell transplantation on vascular remodeling after carotid balloon injury in animal models
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Hong Zou, Yixun Wang, Yan Qi, Wei Jia, Juncang Duan, Jianming Hu, Feng Li, Jin Zhao, Xinxin Ju, Lianghai Wang, Wen Jie Zhang, Kejian Liu, Yutao Wei, Zheng Zhou, Shugang Li, and Lijuan Pang
- Subjects
Oncology ,Neointima ,medicine.medical_specialty ,Pathology ,medicine.medical_treatment ,lcsh:Medicine ,Subgroup analysis ,Vascular Remodeling ,Mesenchymal Stem Cell Transplantation ,Mice ,Internal medicine ,medicine ,Animals ,lcsh:Science ,Multidisciplinary ,business.industry ,Mesenchymal stem cell ,lcsh:R ,Stem-cell therapy ,Clinical trial ,Transplantation ,Disease Models, Animal ,Treatment Outcome ,Meta-analysis ,lcsh:Q ,Rabbits ,Animal studies ,Carotid Artery Injuries ,business ,Research Article - Abstract
Aim A meta-analysis was conducted to assess the efficacy of mesenchymal stem cell (MSC) transplantation in small animal coronary vessels after balloon injury, to provide data for the design of future pre-clinical experiments and human clinical trials. Methods The search strategy included the PubMed, EMBASE, Chinese Biomedical Literature (CBM), and China National Knowledge Infrastructure (CKNI) databases. The endpoint was the ratio of vascular neointima/media (I/M). Moreover, neointimal area, re-endothelialization, and proliferating cell nuclear antigen (PCNA) expression were analyzed. Pooled analyses were conducted using random effects models. Heterogeneity and publication bias were also explored. All data were analyzed using RevMan 5.2 and Stata 12.0. Results Fifteen studies were reviewed from 238 retrieved animal studies. Compared with controls, MSC transplantation resulted in greater I/M reduction (pooled difference, 0.39; 95% CI, 0.57–0.21; P < 0.0001), greater neointimal area reduction (pooled difference, 0.16; 95% CI, 0.22–0.10; P < 0.0001), decreased PCNA expression (pooled difference, 17.69; 95% CI, 28.94–6.44; P = 0.002), and enhanced re-endothelialization (pooled difference, 3.37; 95% CI, 1.78–4.95; P < 0.0001). The multivariable meta-regression analysis showed that a higher number of transplanted cells (>106; P = 0.017) and later time point of I/M measurement (P = 0.022) were significantly associated with I/M reduction. Subgroup analysis demonstrated a trend for a greater reduction in the ratio of I/M with late MSC transplantation (>1 day), MSCs transplanted through intravenous injection, and atherosclerotic vessels. Conclusion The meta-analysis results demonstrate that MSC transplantation might improve injured vascular remodeling. In addition to greater efficacy with a greater number of transplanted MSCs (>106), the long-term effect of MSC transplantation appears to be more significant. The findings of this meta-analysis may help to design future, effective MSC trials.
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- 2015
26. TGF-β1/Smad signaling pathway regulates epithelial-to-mesenchymal transition in esophageal squamous cell carcinoma: in vitro and clinical analyses of cell lines and nomadic Kazakh patients from northwest Xinjiang, China
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Hong Zou, Cuilei Wei, Yutao Wei, Xinxin Ju, Qiuxiang Li, Jiaojiao Lan, Wei Zhao, Wei Jia, Shugang Li, Feng Li, Jianming Hu, Jinfang Jiang, Jianxin Xie, Weiwei Cao, Li Li, Weihua Liang, Yan Qi, Lijuan Pang, Fudong Liu, Jin Zhao, Yang Zhou, Jianwei He, and Chengyan Wang
- Subjects
Adult ,Male ,Cell signaling ,Pathology ,medicine.medical_specialty ,China ,Clinical Pathology ,Epithelial-Mesenchymal Transition ,Esophageal Neoplasms ,lcsh:Medicine ,Vimentin ,Smad Proteins ,SMAD ,Pathology and Laboratory Medicine ,Transforming Growth Factor beta1 ,Antigens, CD ,Cell Line, Tumor ,medicine ,Medicine and Health Sciences ,Humans ,Neoplasm Invasiveness ,Epithelial–mesenchymal transition ,Neoplasm Metastasis ,lcsh:Science ,Aged ,Multidisciplinary ,biology ,Cadherin ,lcsh:R ,Middle Aged ,Cadherins ,Immunohistochemistry ,Kazakhstan ,Tumor progression ,biology.protein ,Cancer research ,Carcinoma, Squamous Cell ,Disease Progression ,Female ,lcsh:Q ,Esophageal Squamous Cell Carcinoma ,Signal transduction ,Molecular Pathology ,Transforming growth factor ,Research Article ,Signal Transduction - Abstract
Invasion and metastasis are the major causes of death in patients with esophageal squamous cell carcinoma (ESCC). Epithelial-mesenchymal transition (EMT) is a critical step in tumor progression and transforming growth factor-β1 (TGF-β1) signaling has been shown to play an important role in EMT. In this study, we investigated how TGF-β1 signaling pathways contributed to EMT in three ESCC cell lines as well as 100 patients of nomadic ethnic Kazakhs residing in northwest Xinjiang Province of China. In vitro analyses included Western blotting to detect the expression of TGF-β1/Smad and EMT-associated proteins in Eca109, EC9706 and KYSE150 cell lines following stimulation with recombinant TGF-β1 and SB431542, a potent inhibitor of ALK5 that also inhibits TGF-β type II receptor. TGF-β-activated Smad2/3 signaling in EMT was significantly upregulated as indicated by mesenchymal markers of N-cadherin and Vimentin, and in the meantime, epithelial marker, E-cadherin, was markedly downregulated. In contrast, SB431542 addition downregulated the expression of N-cadherin and Vimentin, but upregulated the expression of E-cadherin. Moreover, the TGF-β1-induced EMT promoted invasion capability of Eca109 cells. Tumor cells undergoing EMT acquire fibroblastoid-like phenotype. Expressed levels of TGF-β1/Smad signaling molecules and EMT-associated proteins were examined using immunohistochemical analyses in 100 ESCC tissues of Kazakh patients and 58 matched noncancerous adjacent tissues. The results showed that ESCC tissues exhibited upregulated expression of TGF-β1/Smad. We also analyzed the relationship between the above proteins and the patients' clinicopathological characteristics. The TGF-β1/Smad signaling pathway in human Eca109 ESCC cells may carry similar features as in Kazakh ESCC patients, suggesting that TGF-β1/Smad signaling pathway may be involved in the regulation of EMT in ethnic Kazakh patients with ESCC from Xinjiang, China.
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- 2014
27. Metabotropic glutamate receptor 3 is associated with heroin dependence but not depression or schizophrenia in a Chinese population
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Zun-xiao Dai, Wei Jia, Yongsheng Zhu, Feng Zhu, Rui Zhang, Ping-ping Li, and Bin Wu
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Male ,Oncology ,Linkage disequilibrium ,lcsh:Medicine ,Receptors, Metabotropic Glutamate ,Linkage Disequilibrium ,Gene Frequency ,lcsh:Science ,Psychiatry ,Genetics ,Multidisciplinary ,Heroin Dependence ,Substance Abuse ,Middle Aged ,Mental Health ,Behavioral Pharmacology ,Schizophrenia ,Medicine ,Female ,Research Article ,Adult ,Drugs and Devices ,China ,medicine.medical_specialty ,Adolescent ,Genotype ,Single-nucleotide polymorphism ,Biology ,Polymorphism, Single Nucleotide ,Young Adult ,Asian People ,Recreational Drug Use ,Internal medicine ,medicine ,Humans ,Genetic Predisposition to Disease ,Allele ,Allele frequency ,Depressive Disorder ,Population Biology ,Mood Disorders ,Haplotype ,lcsh:R ,Human Genetics ,Odds ratio ,medicine.disease ,Minor allele frequency ,Logistic Models ,Genetic Polymorphism ,lcsh:Q ,Population Genetics - Abstract
Metabotropic glutamate receptor subtype 3 (mGluR3, encoded by GRM3) plays important roles in the pathophysiology of schizophrenia, depression, and drug dependence. GRM3 polymorphisms were reported to be associated with prefrontal activity, cognitive shifting, and memory capability in healthy subjects, as well as susceptibility to schizophrenia and depression. The goal of this study was to replicate the association of GRM3 with schizophrenia and depression and to explore GRM3’s potential association with heroin dependence (HD) in a Chinese population. Seventeen SNPs throughout the GRM3 gene were genotyped using MALDI-TOF within the MassARRAY system, and the allele and genotype distributions were compared between 619 healthy controls and 433 patients with schizophrenia, 409 patients with major depression, and 584 unrelated addicts. We found that GRM3 polymorphisms modulate the susceptibility to HD but do not significantly influence the risk for schizophrenia or depression. An increased risk of HD was significantly associated with the minor alleles of two GRM3 SNPs, including the T allele of rs274618 (Odds ratio (OR) = 1.631, 95% confidence interval (95%CI): 1.317–2.005), the T allele of rs274622 (OR = 1.652, 95% CI: 1.336–2.036), compared with the major alleles. The addicts carrying the minor allele of rs274618 or rs274622 had a shortened duration for transition from first use to dependence (DTFUD) in comparison to homozygote for major allele (P
- Published
- 2014
28. Long-term effects of methadone maintenance treatment with different psychosocial intervention models
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Hengxin Li, Xiaoli Wei, Jin-song Li, Xue-liang Wang, Lirong Wang, and Wei Jia
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Counseling ,Male ,Viral Diseases ,Every Six Months ,lcsh:Medicine ,HIV Infections ,Psychology ,lcsh:Science ,Multidisciplinary ,Incidence (epidemiology) ,Hepatitis C ,Prognosis ,Infectious Diseases ,Mental Health ,Medicine ,Female ,Public Health ,medicine.drug ,Research Article ,Adult ,Narcotics ,medicine.medical_specialty ,Methadone maintenance ,Drugs and Devices ,China ,Medication Therapy Management ,Substance-Related Disorders ,Immunology ,Contingency management ,Models, Psychological ,Internal medicine ,mental disorders ,medicine ,Opiate Substitution Treatment ,Humans ,Biology ,Behavior ,business.industry ,Proportional hazards model ,lcsh:R ,HIV ,medicine.disease ,Adjustment (Psychology) ,Confidence interval ,Surgery ,Clinical Immunology ,lcsh:Q ,business ,Methadone ,Follow-Up Studies - Abstract
This study evaluated the long-term effects of different psychosocial intervention models in methadone maintenance treatment (MMT) in Xi'an China. Patients from five MMT clinics were divided into three groups receiving MMT only, MMT with counseling psychology (CP) or MMT with contingency management (CM). A five-year follow-up was carried out with daily records of medication, monthly random urine morphine tests, and tests for anti-HIV and anti-HCV every six months. Drug use behavior was recorded six months after initial recruitment using a survey. Adjusted RRs and their 95% confidence intervals (CIs) were estimated using an unconditional logistic regression model or a Cox proportional hazard model. A total of 2662 patients were recruited with 797 in MMT, 985 in MMT with CP, and 880 in MMT with CM. Following six months of treatment, the injection rates of MMT with CP and MMT with CM groups were significantly lower than that of MMT (5.1% and 6.9% vs. 16.3%, x2 = 47.093 and 29.908, respectively; P
- Published
- 2014
29. Flow Cytometric Immunophenotyping Is Sensitive for the Early Diagnosis of De Novo Aggressive Natural Killer Cell Leukemia (ANKL): A Multicenter Retrospective Analysis
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Li, Yi, primary, Wei, Jia, additional, Mao, Xia, additional, Gao, Qingping, additional, Liu, Longlong, additional, Cheng, Ping, additional, Liu, Limei, additional, Zhang, Xinhua, additional, Zhang, Ke, additional, Wang, Jin, additional, Zhu, Li, additional, Zhou, Jianfeng, additional, Zhang, Yicheng, additional, Meng, Li, additional, Sun, Hanying, additional, Li, Dengju, additional, Huang, Mei, additional, Huang, Wei, additional, Deng, Jinniu, additional, and Zhang, Donghua, additional
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- 2016
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30. Morphogenetic Studies of the Drosophila DA1 Ventral Olfactory Projection Neuron
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Shen, Hung-Chang, primary, Wei, Jia-Yi, additional, Chu, Sao-Yu, additional, Chung, Pei-Chi, additional, Hsu, Tsai-Chi, additional, and Yu, Hung-Hsiang, additional
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- 2016
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31. Epidemiology and Outcomes of Complicated Skin and Soft Tissue Infections among Inpatients in Southern China from 2008 to 2013
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Li, Xiaoyan, primary, Chen, Yunqin, additional, Gao, Weiguo, additional, Ouyang, Wenwei, additional, Wei, Jia, additional, and Wen, Zehuai, additional
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- 2016
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32. Ribosomal L22-like1 (RPL22L1) Promotes Ovarian Cancer Metastasis by Inducing Epithelial-to-Mesenchymal Transition
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Wu, Nan, primary, Wei, Jia, additional, Wang, Yuhui, additional, Yan, Jinyan, additional, Qin, Ying, additional, Tong, Dandan, additional, Pang, Bo, additional, Sun, Donglin, additional, Sun, Haiming, additional, Yu, Yang, additional, Sun, Wenjing, additional, Meng, Xiangning, additional, Zhang, Chunyu, additional, Bai, Jing, additional, Chen, Feng, additional, Geng, Jingshu, additional, Lee, Ki-Young, additional, Fu, Songbin, additional, and Jin, Yan, additional
- Published
- 2015
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33. Statin use is associated with reduced risk of haematological malignancies: evidence from a meta-analysis
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Yin Jin, Xiao Yi, Shang Zhen, and Wei Jia
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Oncology ,medicine.medical_specialty ,Reduced risk ,Drugs and Devices ,Systematic Reviews ,Clinical Research Design ,Epidemiology ,Oral Medicine ,lcsh:Medicine ,Pharmacology ,Cardiovascular ,Global Health ,Risk Assessment ,Cardiovascular Pharmacology ,Hematologic Cancers and Related Disorders ,Risk Factors ,Internal medicine ,medicine ,Humans ,lcsh:Science ,Multidisciplinary ,business.industry ,lcsh:R ,Hematology ,Statin treatment ,Treatment Outcome ,Meta-analysis ,Hematologic Neoplasms ,Medicine ,Observational study ,lcsh:Q ,Hydroxymethylglutaryl-CoA Reductase Inhibitors ,Meta-Analyses ,business ,Cancer Epidemiology ,Research Article - Abstract
BACKGROUND: Several observational studies have shown that statin use may modify the risk of haematological malignancies. To quantify the association between statin use and risk for haematological malignancies, we performed a detailed meta-analysis of published studies regarding this subject. METHODS: We conducted a systematic search of multiple databases including PubMed, Embase, and Cochrane Library Central database up to July 2013. Fixed-effect and random-effect models were used to estimate summary relative risks (RR) and the corresponding 95% confidence intervals (CIs). Potential sources of heterogeneity were detected by meta-regression. Subgroup analyses and sensitivity analysis were also performed. RESULTS: A total of 20 eligible studies (ten case-control studies, four cohort studies, and six RCTs) reporting 1,139,584 subjects and 15,297 haematological malignancies cases were included. Meta-analysis showed that statin use was associated with a statistically significant 19% reduction in haematological malignancies incidence (RR = 0.81, 95% CI [0.70, 0.92]). During subgroup analyses, statin use was associated with a significantly reduced risk of haematological malignancies among observational studies (RR = 0.79, 95% CI [0.67, 0.93]), but not among RCTs (RR = 0.92, 95% CI [0.77, 1.09]). CONCLUSIONS: Based on this comprehensive meta-analysis, statin use may have chemopreventive effects against haematological malignancies. More studies, especially definitive, randomized chemoprevention trials are needed to confirm this association.
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- 2013
34. Differential Regulation of MAPK Phosphorylation in the Dorsal Hippocampus in Response to Prolonged Morphine Withdrawal-Induced Depressive-Like Symptoms in Mice
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Ying Du, Qiong Zhou, Wei Dang, Rui Liu, Jianhua Wang, Jianguo Shi, Bin Wu, Wei Jia, and Rui Zhang
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MAPK/ERK pathway ,Male ,Hippocampus ,lcsh:Medicine ,Signal transduction ,Social and Behavioral Sciences ,Mice ,Molecular cell biology ,Psychology ,Phosphorylation ,lcsh:Science ,Drug Dependence ,Psychiatry ,Multidisciplinary ,biology ,Morphine ,Depression ,Signaling cascades ,Substance Withdrawal Syndrome ,Mental Health ,Behavioral Pharmacology ,Mitogen-activated protein kinase ,Medicine ,Mitogen-Activated Protein Kinases ,medicine.drug ,Research Article ,medicine.medical_specialty ,Drugs and Devices ,MAPK signaling cascades ,p38 mitogen-activated protein kinases ,Internal medicine ,medicine ,Animals ,Protein kinase A ,Biology ,business.industry ,lcsh:R ,Psychoses ,Mice, Inbred C57BL ,Endocrinology ,Therapies ,biology.protein ,MAPK phosphatase ,lcsh:Q ,business ,Neuroscience - Abstract
Depression is one of the most frequent neuropsychiatric comorbidities associated with opiate addiction. Mitogen activated protein kinase (MAPK) and MAPK phosphatase (MKP) are involved in drug addiction and depression. However, the potential role of MAPK and MKP in depression caused by morphine withdrawal remains unclear. We utilized a mouse model of repeated morphine administration to examine the molecular mechanisms that contribute to prolonged withdrawal induced depressive-like behaviors. Depressive-like behaviors were significant at 1 week after withdrawal and worsened over time. Phospho-ERK (extracellular signal-regulated protein kinase) was decreased and MKP-1 was elevated in the hippocampus, and JNK (c-Jun N-terminal protein kinase), p38 (p38 protein kinase) and MKP-3 were unaffected. A pharmacological blockade of MKP-1 by intra-hippocampal sanguinarine (SA) infusion prevented the development of depressive-like behaviors and resulted in relatively normal levels of MKP-1 and phospho-ERK after withdrawal. Our findings support the association between hippocampal MAPK phosphorylation and prolonged morphine withdrawal-induced depression, and emphasize the MKP-1 as an negative regulator of the ERK phosphorylation that contributes to depression.
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- 2013
35. High fat diet feeding exaggerates perfluorooctanoic acid-induced liver injury in mice via modulating multiple metabolic pathways
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Guoxiang Xie, Xiaobing Tan, Xiuhua Sun, Peter Qiao, Wei Zhong, Zhanxiang Zhou, Xinguo Sun, Wei Jia, and Qiong Li
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Male ,Adipose tissue ,Toxicology ,chemistry.chemical_compound ,Mice ,chemistry.chemical_classification ,Liver injury ,Fluorocarbons ,Multidisciplinary ,biology ,Liver Diseases ,Fatty liver ,Fatty Acids ,Alanine Transaminase ,Organ Size ,medicine.anatomical_structure ,Liver ,Hepatocyte ,Perfluorooctanoic acid ,Medicine ,Public Health ,Caprylates ,Environmental Health ,Research Article ,medicine.medical_specialty ,Science ,Adipose Tissue, White ,Toxic Agents ,Predictive Toxicology ,Gastroenterology and Hepatology ,Diet, High-Fat ,Necrosis ,Internal medicine ,medicine ,Animals ,Aspartate Aminotransferases ,Biology ,Triglycerides ,Nutrition ,Inflammation ,Gene Expression Profiling ,Body Weight ,Fatty acid ,Lipid metabolism ,medicine.disease ,Lipid Metabolism ,Fatty Liver ,Mice, Inbred C57BL ,Endocrinology ,chemistry ,Alanine transaminase ,Gene Expression Regulation ,Metabolic Disorders ,biology.protein ,Hepatocytes - Abstract
High fat diet (HFD) is closely linked to a variety of health issues including fatty liver. Exposure to perfluorooctanoic acid (PFOA), a synthetic perfluorinated carboxylic acid, also causes liver injury. The present study investigated the possible interactions between high fat diet and PFOA in induction of liver injury. Mice were pair-fed a high-fat diet (HFD) or low fat control with or without PFOA administration at 5 mg/kg/day for 3 weeks. Exposure to PFOA alone caused elevated plasma alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels and increased liver weight along with reduced body weight and adipose tissue mass. HFD alone did not cause liver damage, but exaggerated PFOA-induced hepatotoxicity as indicated by higher plasma ALT and AST levels, and more severe pathological changes including hepatocyte hypertrophy, lipid droplet accumulation and necrosis as well as inflammatory cell infiltration. These additive effects of HFD on PFOA-induced hepatotoxicity correlated with metabolic disturbance in liver and blood as well as up-regulation of hepatic proinflammatory cytokine genes. Metabolomic analysis demonstrated that both serum and hepatic metabolite profiles of PFOA, HFD, or HFD-PFOA group were clearly differentiated from that of controls. PFOA affected more hepatic metabolites than HFD, but HFD showed positive interaction with PFOA on fatty acid metabolites including long chain fatty acids and acylcarnitines. Taken together, dietary high fat potentiates PFOA-induced hepatic lipid accumulation, inflammation and necrotic cell death by disturbing hepatic metabolism and inducing inflammation. This study demonstrated, for the first time, that HFD increases the risk of PFOA in induction of hepatotoxicity.
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- 2012
36. Fur in Magnetospirillum gryphiswaldense influences magnetosomes formation and directly regulates the genes involved in iron and oxygen metabolism
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Jian Li, Chengbo Rong, Jiangning Liu, Wei-Jia Zhang, Lei Qi, Ying Li, and Long-Fei Wu
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Iron ,Magnetosome ,Mutant ,Gene Expression ,Repressor ,lcsh:Medicine ,Biology ,Microbiology ,chemistry.chemical_compound ,Bacterial Proteins ,Drug Resistance, Bacterial ,Molecular Cell Biology ,Genetics ,medicine ,Magnetospirillum ,Promoter Regions, Genetic ,lcsh:Science ,Multidisciplinary ,Chromosome Biology ,lcsh:R ,Bacteriology ,Gene Expression Regulation, Bacterial ,Hydrogen Peroxide ,Genomics ,biology.organism_classification ,Oxygen ,Repressor Proteins ,Streptonigrin ,Biochemistry ,chemistry ,Genes, Bacterial ,Ferric ,Epigenetics ,lcsh:Q ,Magnetosomes ,Chromatin immunoprecipitation ,Metabolic Networks and Pathways ,Intracellular ,Protein Binding ,Research Article ,Biotechnology ,medicine.drug - Abstract
Magnetospirillum gryphiswaldense strain MSR-1 has the unique capability of taking up large amounts of iron and synthesizing magnetosomes (intracellular magnetic particles composed of Fe(3)O(4)). The unusual high iron content of MSR-1 makes it a useful model for studying biological mechanisms of iron uptake and homeostasis. The ferric uptake regulator (Fur) protein plays a key role in maintaining iron homeostasis in many bacteria. We identified and characterized a fur-homologous gene (MGR_1314) in MSR-1. MGR_1314 was able to complement a fur mutant of E. coli in iron-responsive manner in vivo. We constructed a fur mutant strain of MSR-1. In comparison to wild-type MSR-1, the mutant strain had lower magnetosome formation, and was more sensitive to hydrogen peroxide and streptonigrin, indicating higher intracellular free iron content. Quantitative real-time RT-PCR and chromatin immunoprecipitation analyses indicated that Fur protein directly regulates expression of several key genes involved in iron transport and oxygen metabolism, in addition it also functions in magnetosome formation in M. gryphiswaldense.
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- 2012
37. The Features of Genetic Prion Diseases Based on Chinese Surveillance Program
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Shi, Qi, primary, Zhou, Wei, additional, Chen, Cao, additional, Zhang, Bao-Yun, additional, Xiao, Kang, additional, Zhang, Xiu-Chun, additional, Shen, Xiao-Jing, additional, Li, Qing, additional, Deng, Li-Quan, additional, Dong, Jian-Hua, additional, Lin, Wen-Qing, additional, Huang, Pu, additional, Jiang, Wei-Jia, additional, Lv, Jie, additional, Han, Jun, additional, and Dong, Xiao-Ping, additional
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- 2015
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38. Efficacy of Mesenchymal Stem Cell Therapy for Steroid-Refractory Acute Graft-Versus-Host Disease following Allogeneic Hematopoietic Stem Cell Transplantation: A Systematic Review and Meta-Analysis
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Chen, Xiaomei, primary, Wang, Chunyan, additional, Yin, Jin, additional, Xu, Jinhuan, additional, Wei, Jia, additional, and Zhang, Yicheng, additional
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- 2015
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39. Data-Driven Information Extraction from Chinese Electronic Medical Records
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Xu, Dong, primary, Zhang, Meizhuo, additional, Zhao, Tianwan, additional, Ge, Chen, additional, Gao, Weiguo, additional, Wei, Jia, additional, and Zhu, Kenny Q., additional
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- 2015
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40. Quantification of EUGR as a Measure of the Quality of Nutritional Care of Premature Infants
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Lin, Zhenlang, primary, Green, Robert S., additional, Chen, Shangqin, additional, Wu, Hui, additional, Liu, Tiantian, additional, Li, Jingyang, additional, Wei, Jia, additional, and Lin, Jing, additional
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- 2015
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41. Identification of Pathogen Signatures in Prostate Cancer Using RNA-seq
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Chen, Yunqin, primary and Wei, Jia, additional
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- 2015
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42. BTNL2 Gene Polymorphism and Sarcoidosis Susceptibility: A Meta-Analysis
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Lin, Yihua, primary, Wei, Jia, additional, Fan, Lili, additional, and Cheng, Deyun, additional
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- 2015
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43. Psychometric Properties of the Chinese Version of the Fear of Negative Evaluation Scale-Brief (BFNE) and the BFNE-Straightforward for Middle School Students
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Wei, Jia, primary, Zhang, Chunyu, additional, Li, Yadan, additional, Xue, Song, additional, and Zhang, Jinfu, additional
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- 2015
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44. Construction of Drug Network Based on Side Effects and Its Application for Drug Repositioning
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Ye, Hao, primary, Liu, Qi, additional, and Wei, Jia, additional
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- 2014
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45. Incidence and Risk of Cardiotoxicity Associated with Bortezomib in the Treatment of Cancer: A Systematic Review and Meta-Analysis
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Xiao, Yi, primary, Yin, Jin, additional, Wei, Jia, additional, and Shang, Zhen, additional
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- 2014
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46. MicroRNA-92a as a Potential Biomarker in Diagnosis of Colorectal Cancer: A Systematic Review and Meta-Analysis
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Manran Liu, Xiaoyan Yi, Chao Gao, Taixian Yuan, Wei Jia, Zongyue Zeng, Xin Yang, and Yixuan Hou
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Oncology ,Health Screening ,Colorectal cancer ,lcsh:Medicine ,Bioinformatics ,Biochemistry ,Nucleic Acids ,Gastrointestinal Cancers ,Pathology ,Medicine ,lcsh:Science ,Multidisciplinary ,Area under the curve ,Middle Aged ,Meta-analysis ,Regression Analysis ,Biomarker (medicine) ,Public Health ,Colorectal Neoplasms ,Research Article ,Test Evaluation ,medicine.medical_specialty ,Systematic Reviews ,Clinical Research Design ,Gastroenterology and Hepatology ,Genetic Heterogeneity ,Diagnostic Medicine ,Internal medicine ,Gastrointestinal Tumors ,Biomarkers, Tumor ,Cancer Detection and Diagnosis ,Humans ,Biology ,Aged ,Receiver operating characteristic ,business.industry ,lcsh:R ,Cancers and Neoplasms ,Publication bias ,medicine.disease ,Confidence interval ,MicroRNAs ,ROC Curve ,Diagnostic odds ratio ,RNA ,lcsh:Q ,Meta-Analyses ,business ,Publication Bias ,Biomarkers ,General Pathology - Abstract
Introduction Previous studies demonstrated that MicroRNA-92a (miR-92a) was significantly differential expressed between colorectal cancer (CRC) patients and control cohorts, which provide timely relevant evidence for miR-92a as a novel promising biomarker in the colorectal cancer patients. This meta-analysis aimed to evaluate potential diagnostic value of plasma miR-92a. Methods Relevant literatures were collected in PubMed, Embase, Chinese Biomedical Literature Database (CBM), Chinese National Knowledge Infrastructure (CNKI) and Technology of Chongqing (VIP), and Wan Fang Data. Sensitivity, specificity and diagnostic odds ratio (DOR) for miR-92a in the diagnosis of CRC were pooled using random effects models. Summary receiver operating characteristic (SROC) curve analysis and the area under the curve (AUC) were used to estimate the overall test performance. Results This Meta-analysis included six studies with a total of 521 CRC patients and 379 healthy controls. For miR-92a, the pooled sensitivity, specificity and DOR to predict CRC patients were 76% (95% confidence interval [CI]: 72%–79%), 64% (95% confidence interval [CI]: 59%–69%) and 8.05 (95% CI: 3.50–18.56), respectively. In addition, the AUC of miR-92a in diagnosis CRC is 0.7720. Conclusions MicroRNA-92a might be a novel potential biomarker in the diagnosis of colorectal cancer, and more studies are needed to highlight the theoretical strengths.
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- 2014
47. SULF2 Methylation Is Associated with In Vitro Cisplatin Sensitivity and Clinical Efficacy for Gastric Cancer Patients Treated with a Modified FOLFOX Regimen
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Shen, Jie, primary, Wei, Jia, additional, Wang, Hao, additional, Yang, Yang, additional, Yue, Guofeng, additional, Wang, Lin, additional, Yu, Lixia, additional, Xie, Li, additional, Sun, Xia, additional, Bian, Xinyu, additional, Zou, Zhengyun, additional, Qian, Xiaoping, additional, Guan, Wenxian, additional, and Liu, Baorui, additional
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- 2013
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48. Synergistic Anticancer Effects of Polyphyllin I and Evodiamine on Freshly-Removed Human Gastric Tumors
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Yue, Guofeng, primary, Wei, Jia, additional, Qian, Xiaoping, additional, Yu, Lixia, additional, Zou, Zhengyun, additional, Guan, Wenxian, additional, Wang, Hao, additional, Shen, Jie, additional, and Liu, Baorui, additional
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- 2013
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49. Increased p66Shc in the Inner Ear of D-Galactose-Induced Aging Mice with Accumulation of Mitochondrial DNA 3873-bp Deletion: p66Shc and mtDNA Damage in the Inner Ear during Aging
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Wu, Lisa, primary, Sun, Yu, additional, Hu, Yu-Juan, additional, Yang, Yang, additional, Yao, Ling-Li, additional, Zhou, Xing-Xing, additional, Wang, Hao, additional, Zhang, Rui, additional, Huang, Xiang, additional, and Kong, Wei-Jia, additional
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- 2012
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50. The Crystal Structure of Arabidopsis VSP1 Reveals the Plant Class C-Like Phosphatase Structure of the DDDD Superfamily of Phosphohydrolases
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Chen, Yuhong, primary, Wei, Jia, additional, Wang, Mingzhu, additional, Shi, Zhubing, additional, Gong, Weimin, additional, and Zhang, Min, additional
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- 2012
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