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2 results on '"Tomohiko Katayama"'

1. New type of Sendai virus vector provides transgene-free iPS cells derived from chimpanzee blood.

Author

Yasumitsu Fujie, Noemi Fusaki, Tomohiko Katayama, Makoto Hamasaki, Yumi Soejima, Minami Soga, Hiroshi Ban, Mamoru Hasegawa, Satoshi Yamashita, Shigemi Kimura, Saori Suzuki, Tetsuro Matsuzawa, Hirofumi Akari, and Takumi Era

Subjects
Medicine, Science
Abstract

Induced pluripotent stem cells (iPSCs) are potentially valuable cell sources for disease models and future therapeutic applications; however, inefficient generation and the presence of integrated transgenes remain as problems limiting their current use. Here, we developed a new Sendai virus vector, TS12KOS, which has improved efficiency, does not integrate into the cellular DNA, and can be easily eliminated. TS12KOS carries KLF4, OCT3/4, and SOX2 in a single vector and can easily generate iPSCs from human blood cells. Using TS12KOS, we established iPSC lines from chimpanzee blood, and used DNA array analysis to show that the global gene-expression pattern of chimpanzee iPSCs is similar to those of human embryonic stem cell and iPSC lines. These results demonstrated that our new vector is useful for generating iPSCs from the blood cells of both human and chimpanzee. In addition, the chimpanzee iPSCs are expected to facilitate unique studies into human physiology and disease.

Published
2014
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2. New Type of Sendai Virus Vector Provides Transgene-Free iPS Cells Derived from Chimpanzee Blood

Author

Noemi Fusaki, Makoto Hamasaki, Hirofumi Akari, Yumi Soejima, Takumi Era, Hiroshi Ban, Saori Suzuki, Minami Soga, Mamoru Hasegawa, Yasumitsu Fujie, Satoshi Yamashita, Tetsuro Matsuzawa, Shigemi Kimura, and Tomohiko Katayama

Subjects
Pan troglodytes, Science, Transgene, Cell, Induced Pluripotent Stem Cells, Genetic Vectors, Gene Expression, Pathology and Laboratory Medicine, Microbiology, Sendai virus, Cell Line, Kruppel-Like Factor 4, SOX2, Animal Cells, Transduction, Genetic, medicine, Medicine and Health Sciences, Animals, Humans, Transgenes, Induced pluripotent stem cell, Microbial Pathogens, Multidisciplinary, biology, Stem Cells, Biology and Life Sciences, Cell Differentiation, Cell Biology, biology.organism_classification, Cellular Reprogramming, Embryonic stem cell, Virology, Cell biology, medicine.anatomical_structure, KLF4, Medical Microbiology, Viral Pathogens, Paramyxoviruses, Medicine, DNA microarray, Cellular Types, Pathogens, Research Article
Abstract

Induced pluripotent stem cells (iPSCs) are potentially valuable cell sources for disease models and future therapeutic applications; however, inefficient generation and the presence of integrated transgenes remain as problems limiting their current use. Here, we developed a new Sendai virus vector, TS12KOS, which has improved efficiency, does not integrate into the cellular DNA, and can be easily eliminated. TS12KOS carries KLF4, OCT3/4, and SOX2 in a single vector and can easily generate iPSCs from human blood cells. Using TS12KOS, we established iPSC lines from chimpanzee blood, and used DNA array analysis to show that the global gene-expression pattern of chimpanzee iPSCs is similar to those of human embryonic stem cell and iPSC lines. These results demonstrated that our new vector is useful for generating iPSCs from the blood cells of both human and chimpanzee. In addition, the chimpanzee iPSCs are expected to facilitate unique studies into human physiology and disease.

Published
2014

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