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New Type of Sendai Virus Vector Provides Transgene-Free iPS Cells Derived from Chimpanzee Blood
- Source :
- PLoS ONE, PLoS ONE, Vol 9, Iss 12, p e113052 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science, 2014.
-
Abstract
- Induced pluripotent stem cells (iPSCs) are potentially valuable cell sources for disease models and future therapeutic applications; however, inefficient generation and the presence of integrated transgenes remain as problems limiting their current use. Here, we developed a new Sendai virus vector, TS12KOS, which has improved efficiency, does not integrate into the cellular DNA, and can be easily eliminated. TS12KOS carries KLF4, OCT3/4, and SOX2 in a single vector and can easily generate iPSCs from human blood cells. Using TS12KOS, we established iPSC lines from chimpanzee blood, and used DNA array analysis to show that the global gene-expression pattern of chimpanzee iPSCs is similar to those of human embryonic stem cell and iPSC lines. These results demonstrated that our new vector is useful for generating iPSCs from the blood cells of both human and chimpanzee. In addition, the chimpanzee iPSCs are expected to facilitate unique studies into human physiology and disease.
- Subjects :
- Pan troglodytes
Science
Transgene
Cell
Induced Pluripotent Stem Cells
Genetic Vectors
Gene Expression
Pathology and Laboratory Medicine
Microbiology
Sendai virus
Cell Line
Kruppel-Like Factor 4
SOX2
Animal Cells
Transduction, Genetic
medicine
Medicine and Health Sciences
Animals
Humans
Transgenes
Induced pluripotent stem cell
Microbial Pathogens
Multidisciplinary
biology
Stem Cells
Biology and Life Sciences
Cell Differentiation
Cell Biology
biology.organism_classification
Cellular Reprogramming
Embryonic stem cell
Virology
Cell biology
medicine.anatomical_structure
KLF4
Medical Microbiology
Viral Pathogens
Paramyxoviruses
Medicine
DNA microarray
Cellular Types
Pathogens
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....55997412c77d95655c3541c76687b3b8