1. Transplantation of chicken egg white extract-induced rabbit PBMCs as a treatment for renal ischemia-reperfusion injury in rabbits
- Author
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Xue-Min Cai, Ruan Guangping, Xiang Yao, Rong-Qing Pang, Qing-Keng Lin, Zi-an Li, and Xing-Hua Pan
- Subjects
Cell Extracts ,0301 basic medicine ,Physiology ,Cell Transplantation ,Eggs ,medicine.medical_treatment ,Kidney ,Vascular Medicine ,chemistry.chemical_compound ,Multipotency ,0302 clinical medicine ,Reproductive Physiology ,Ischemia ,Animal Cells ,Medicine and Health Sciences ,Renal Transplantation ,Blood and Lymphatic System Procedures ,Medicine ,Cells, Cultured ,Kidney transplantation ,Mammals ,Multidisciplinary ,Stem Cell Therapy ,Stem Cells ,Acute kidney injury ,Eukaryota ,Cell Differentiation ,Animal Models ,Stem-cell therapy ,Bird Eggs ,Experimental Organism Systems ,Reperfusion Injury ,030220 oncology & carcinogenesis ,Vertebrates ,Leporids ,Chicken Eggs ,Rabbits ,Anatomy ,Cellular Types ,Renal Ischemia ,Research Article ,Pluripotent Stem Cells ,Cell Potency ,Science ,Renal function ,Surgical and Invasive Medical Procedures ,Research and Analysis Methods ,Peripheral blood mononuclear cell ,Urinary System Procedures ,Andrology ,03 medical and health sciences ,Egg White ,Animals ,Clinical Genetics ,Transplantation ,Creatinine ,Renal ischemia ,business.industry ,Organisms ,Biology and Life Sciences ,Kidneys ,Renal System ,Organ Transplantation ,Cell Biology ,medicine.disease ,030104 developmental biology ,chemistry ,Amniotes ,Animal Studies ,Leukocytes, Mononuclear ,business ,Zoology ,Stem Cell Transplantation - Abstract
Ischemia-reperfusion injury is an important contributor to acute kidney injury and a major factor affecting early functional recovery after kidney transplantation. We conducted this experiment to investigate the protective effect of induced multipotent stem cell transplantation on renal ischemia-reperfusion injury. Forty rabbits were divided into four groups of 10 rabbits each. Thirty rabbits were used to establish the renal ischemia-reperfusion injury model, and ten rabbits served as the model group and were not treated. Among the 30 rabbits with renal ischemia-reperfusion injury, 10 rabbits were treated with induced peripheral blood mononuclear cells (PBMCs), and 10 other rabbits were treated with noninduced PBMCs. After three weekly treatments, the serum creatinine levels, urea nitrogen levels and urine protein concentrations were quantified. The kidneys were stained with hematoxylin-eosin (HE), periodic acid-Schiff (PAS) and Masson’s trichrome and then sent for commercial metabolomic testing. The kidneys of the rabbits in the model group showed different degrees of pathological changes, and the recovery of renal function was observed in the group treated with induced cells. The results indicate that PBMCs differentiate into multipotent stem cells after induction and exert a therapeutic effect on renal ischemia-reperfusion injury.
- Published
- 2020