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5 results on '"Ikeda, Tadashi"'

1. In vivo recellularization of xenogeneic vascular grafts decellularized with high hydrostatic pressure method in a porcine carotid arterial interpose model

Author

Kurokawa, Shunji, primary, Hashimoto, Yoshihide, additional, Funamoto, Seiichi, additional, Murata, Kozue, additional, Yamashita, Akitatsu, additional, Yamazaki, Kazuhiro, additional, Ikeda, Tadashi, additional, Minatoya, Kenji, additional, Kishida, Akio, additional, and Masumoto, Hidetoshi, additional

Published
2021
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2. Correction: Efficient and robust differentiation of endothelial cells from human induced pluripotent stem cells via lineage control with VEGF and cyclic AMP

Author

Ikuno, Takeshi, Masumoto, Hidetoshi, Yamamizu, Kohei, Yoshioka, Miki, Minakata, Kenji, Ikeda, Tadashi, Sakata, Ryuzo, and Yamashita, Jun K.

Subjects
Vascular Endothelial Growth Factor A, Multidisciplinary, Induced Pluripotent Stem Cells, lcsh:R, Correction, lcsh:Medicine, Cell Differentiation, Flow Cytometry, Cyclic AMP, Human Umbilical Vein Endothelial Cells, Humans, Cell Lineage, lcsh:Q, lcsh:Science
Abstract

Blood vessels are essential components for many tissues and organs. Thus, efficient induction of endothelial cells (ECs) from human pluripotent stem cells is a key method for generating higher tissue structures entirely from stem cells. We previously established an EC differentiation system with mouse pluripotent stem cells to show that vascular endothelial growth factor (VEGF) is essential to induce ECs and that cyclic adenosine monophosphate (cAMP) synergistically enhances VEGF effects. Here we report an efficient and robust EC differentiation method from human pluripotent stem cell lines based on a 2D monolayer, serum-free culture. We controlled the direction of differentiation from mesoderm to ECs using stage-specific stimulation with VEGF and cAMP combined with the elimination of non-responder cells at early EC stage. This "stimulation-elimination" method robustly achieved very high efficiency (99%) and yield (10 ECs from 1 hiPSC input) of EC differentiation, with no purification of ECs after differentiation. We believe this method will be a valuable technological basis broadly for regenerative medicine and 3D tissue engineering.

Published
2017

5. Induction and Enhancement of Cardiac Cell Differentiation from Mouse and Human Induced Pluripotent Stem Cells with Cyclosporin-A

Author

Fujiwara, Masataka, primary, Yan, Peishi, additional, Otsuji, Tomomi G., additional, Narazaki, Genta, additional, Uosaki, Hideki, additional, Fukushima, Hiroyuki, additional, Kuwahara, Koichiro, additional, Harada, Masaki, additional, Matsuda, Hiroyuki, additional, Matsuoka, Satoshi, additional, Okita, Keisuke, additional, Takahashi, Kazutoshi, additional, Nakagawa, Masato, additional, Ikeda, Tadashi, additional, Sakata, Ryuzo, additional, Mummery, Christine L., additional, Nakatsuji, Norio, additional, Yamanaka, Shinya, additional, Nakao, Kazuwa, additional, and Yamashita, Jun K., additional

Published
2011
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