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10 results on '"Ianora, Adrianna"'

1. Zebrafish-based identification of the antiseizure nucleoside inosine from the marine diatom Skeletonema marinoi

Author

Brillatz, Théo, primary, Lauritano, Chiara, additional, Jacmin, Maxime, additional, Khamma, Supitcha, additional, Marcourt, Laurence, additional, Righi, Davide, additional, Romano, Giovanna, additional, Esposito, Francesco, additional, Ianora, Adrianna, additional, Queiroz, Emerson F., additional, Wolfender, Jean-Luc, additional, and Crawford, Alexander D., additional

Published
2018
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10. High-quality RNA extraction from the sea urchin Paracentrotus lividus embryos

Author

Giovanna Romano, Angelo Fontana, Susan Costantini, Maria Costantini, Nadia Ruocco, Valerio Zupo, Adrianna Ianora, Ruocco, Nadia, Costantini, Susan, Zupo, Valerio, Romano, Giovanna, Ianora, Adrianna, Fontana, A, and Costantini, Maria

Subjects
0301 basic medicine, Embryology, Embryo, Nonmammalian, Hydrolases, Molecular biology, lcsh:Medicine, 010501 environmental sciences, Biochemistry, 01 natural sciences, Sequencing techniques, Paracentrotus, DNA sequencing, lcsh:Science, Sea urchin, Deoxyribonucleases, Multidisciplinary, biology, Animal Models, Genomics, Enzymes, Nucleic acids, RNA isolation, Experimental Organism Systems, Female, RNA extraction, Transcriptome Analysis, Research Article, Echinoderms, Next-Generation Sequencing, food.ingredient, Nucleases, RNA integrity number, Biomolecular isolation, Paracentrotus lividus, 03 medical and health sciences, Extraction techniques, Model Organisms, food, biology.animal, Chemical Biology, DNA-binding proteins, Botany, Mediterranean Sea, Genetics, Animals, 14. Life underwater, Pluteus, Non-coding RNA, 0105 earth and related environmental sciences, Diatoms, Life Cycle Stages, lcsh:R, Embryos, Organisms, Biology and Life Sciences, Proteins, Computational Biology, RNA, Genome Analysis, biology.organism_classification, Invertebrates, Gene regulation, Research and analysis methods, MicroRNAs, Molecular biology techniques, 030104 developmental biology, Sea Urchins, Enzymology, lcsh:Q, Gene expression, Developmental Biology
Abstract

The sea urchin Paracentrotus lividus (Lamarck, 1816) is a keystone herbivore in the Mediterranean Sea due to its ability to transform macroalgal-dominated communities into barren areas characterized by increased cover of bare substrates and encrusting coralline algae, reduced biodiversity and altered ecosystem functions. P. lividus is also an excellent animal model for toxicology, physiology and biology investigations having been used for more than a century as a model for embryological studies with synchronously developing embryos which are easy to manipulate and analyze for morphological aberrations. Despite its importance for the scientific community, the complete genome is still not fully annotated. To date, only a few molecular tools are available and a few Next Generation Sequencing (NGS) studies have been performed. Here we aimed at setting-up an RNA extraction method to obtain high quality and sufficient quantity of RNA for NGS from P. lividus embryos at the pluteus stage. We compared five different RNA extraction protocols from four different pools of plutei (500, 1000, 2500 and 5000 embryos): TRIzol, and four widely-used Silica Membrane kits, GenElute TM Mammalian Total RNA Miniprep Kit, RNAqueous Micro Kit, RNeasy Micro Kit and Aurum TM Total RNA Mini Kit. The quantity of RNA isolated was evaluated using NanoDrop. The quality, considering the purity, was measured as A260/A280 and A260/230 ratios. The integrity was measured by RNA Integrity Number (RIN). Our results demonstrated that the most efficient procedures were GenElute, RNeasy and Aurum, producing a sufficient quantity of RNA for NGS. The Bioanalyzer profiles and RIN values revealed that the most efficient methods guaranteeing for RNA integrity were RNeasy and Aurum combined with an initial preservation in RNAlater. This research represents the first attempt to standardize a method for high-quality RNA extraction from sea urchin embryos at the pluteus stage, providing a new resource for this established model marine organism.

Published
2017

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