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2. High-dose radiation-induced immunogenic cell death of bladder cancer cells leads to dendritic cell activation.

Author

Zeng, Xianlin, Luo, Daiqin, Zhang, Shuai, Cui, Zhonghui, Wang, Yun, Chen, Jin, Zhang, Shichao, Teng, Lijing, Hu, Zuquan, Liu, Lina, Zhou, Shi, Zeng, Zhu, and Long, Jinhua

Subjects
CELL cycle, DENDRITIC cells, CELL death, CANCER cells, CANCER prognosis
Abstract

Radiotherapy is a commonly used method in the treatment of bladder cancers (BC). Radiation-induced immunogenic cell death (ICD) is related to the immune response against cancers and their prognoses. Even though dendritic cells (DC) act as powerful antigen-presenting cells in the body, their precise role in this ICD process remains unclear. Accordingly, an in vitro study was undertaken to ascertain whether high-dose radiation-induced ICD of BC cells could regulate the immune response of DC. The results indicated that high-dose radiation treatments of BC cells significantly increased their levels of apoptosis, blocked their cell cycle in the G2/M phase, increased their expression of ICD-related proteins, and upregulated their secretion of CCL5 and CCL21 which control the directed migration of DC. It was also noted that expression of CD80, CD86, CCR5, and CCR7 on DC was upregulated in the medium containing the irradiated cells. In conclusion, the present findings illustrate that high-dose radiation can induce the occurrence of ICD within BC cells, concomitantly resulting in the activation of DC. Such findings could be of great significance in increasing the understanding how radiotherapy of BC may work to bring about reductions in cell activity and how these processes in turn lead to immunoregulation of the function of DC. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Effect of NaCl-stressed Bacillus thuringiensis (Bt) cotton on the feeding behaviors and nutritional parameters of Helicoverpa armigera

Author

Li-Juan Zhang, Jun-Yu Luo, Jin-Jie Cui, Zhang Kaixin, Zhu Xiangzhen, Li Wang, Jichao Ji, Zhang Shuai, and Wang Chunyi

Subjects
0106 biological sciences, Insecticides, Bacillus thuringiensis, lcsh:Medicine, Genetically modified crops, Helicoverpa armigera, Moths, Sodium Chloride, 01 natural sciences, Salt Stress, Animal science, Bacterial Proteins, Relative growth rate, Animals, Herbivory, lcsh:Science, Pest Control, Biological, Gossypium, Multidisciplinary, biology, business.industry, lcsh:R, fungi, Pest control, biology.organism_classification, Plants, Genetically Modified, 010602 entomology, Bollworm, Bt cotton, Instar, lcsh:Q, business, 010606 plant biology & botany
Abstract

Saline-alkali soil is an arable land resource on which transgenic Bacillus thuringiensis (Bt) cotton has been planted on a large scale in accordance with food security strategies. There are, however, concerns about the insecticidal effects of Bt cotton on target insect pests. In this study, a Bt cotton variety, GK19, and its nontransgenic parent variety, Simian-3, were used as experimental models for investigating the effect of the expression of exogenous insecticidal proteins in Bt cotton under NaCl stress on the feeding behavior and nutritional parameters of Helicoverpa armigera. The results showed that the expression of exogenous insecticidal proteins in GK19 was significantly inhibited under NaCl stress. However, the feeding, crawling, resting and spinning down behavior of the 5th instar H. armigera larvae on GK19 Bt cotton, as well as the amount of food consumed and feces produced by these larvae, did not markedly differ under different NaCl concentrations. In contrast, the mean relative growth rate (MRGR), relative growth rate (RGR), approximate digestibility (AD), efficiency of conversion of ingested food (ECI) and efficiency of conversion of digested food (ECD) of the larvae markedly decreased in response to NaCl stress. Under the same concentration of NaCl, the nutritional parameters of the bollworm larvae on GK19 Bt cotton or Simian-3 nontransgenic cotton were different. However, the interaction between salt stress and cotton variety had no significant effect on the feeding behavior or nutritional parameters of H. armigera larvae. These results may provide a scientific basis for determining the effect of exogenous insecticidal protein expression in Bt cotton under NaCl stress on H. armigera and can therefore be useful for the effective application of Bt cotton in saline-alkali soils to prevent and control H. armigera.

Published
2018

13. Identification of candidate chemosensory genes by transcriptome analysis in Loxostege sticticalis Linnaeus

Author

Kebin Li, Yazhong Cao, Hong-Shuang Wei, Jiao Yin, and Zhang Shuai

Subjects
0301 basic medicine, Male, Life Cycles, Sensory Receptors, Odorant binding, Physiology, Social Sciences, lcsh:Medicine, Receptors, Odorant, Biochemistry, Ion Channels, Transcriptome, Larvae, Medicine and Health Sciences, Psychology, Animal Anatomy, Receptor, lcsh:Science, Musculoskeletal System, Phylogeny, Data Management, Genetics, Multidisciplinary, biology, Physics, Phylogenetic Analysis, Genomics, Biological Evolution, Receptors, Pheromone, Lepidoptera, Electrophysiology, Phylogenetics, Larva, Physical Sciences, Pheromone, Insect Proteins, Olfactory Receptors, Legs, Female, Sensory Perception, Anatomy, Research Article, Signal Transduction, Arthropod Antennae, Computer and Information Sciences, Loxostege sticticalis, Biophysics, Neurophysiology, Receptors, Ionotropic Glutamate, Odorant Binding Proteins, 03 medical and health sciences, Animals, Animal Physiology, Evolutionary Systematics, Pheromone binding, Gene Prediction, Taxonomy, Evolutionary Biology, Gene Expression Profiling, Limbs (Anatomy), fungi, lcsh:R, Biology and Life Sciences, Computational Biology, Proteins, Molecular Sequence Annotation, Cell Biology, Ligand-Gated Ion Channels, biology.organism_classification, Genome Analysis, 030104 developmental biology, Gene Ontology, Membrane protein, Odorant-binding protein, biology.protein, lcsh:Q, Antennae (Animal Physiology), Zoology, Neuroscience, Developmental Biology
Abstract

Loxostege sticticalis Linnaeus is an economically important agricultural pest, and the larvae cause great damage to crops, especially in Northern China. However, effective and environmentally friendly chemical methods for controlling this pest have not been discovered to date. In the present study, we performed HiSeq2500 sequencing of transcriptomes of the male and female adult antennae, adult legs and third instar larvae, and we identified 54 candidate odorant receptors (ORs), including 1 odorant receptor coreceptor (Orco) and 5 pheromone receptors (PRs), 18 ionotropic receptors (IRs), 13 gustatory receptors (GRs), 34 odorant binding proteins (OBPs), including 1 general odorant binding protein (GOBP1) and 3 pheromone binding proteins (PBPs), 10 chemosensory proteins (CSPs) and 2 sensory neuron membrane proteins (SNMPs). The results of RNA-Seq and RT-qPCR analyses showed the expression levels of most genes in the antennae were higher than that in the legs and larvae. Furthermore, PR4, OR1-4, 7-11, 13-15, 23, 29-32, 34, 41, 43, 47/IR7d.2/GR5b, 45, 7/PBP2-3, GOBP1, OBP3, 8 showed female antennae-biased expression, while PR1/OBP2, 7/IR75d/CSP2 showed male antennae-biased expression. However, IR1, 7d.3, 68a/OBP11, 20-22, 28/CSP9 had larvae enriched expression, and OBP15, 17, 25, 29/CSP5 were mainly expressed in the legs. The results shown above indicated that these genes might play a key role in foraging, seeking mates and host recognition in the L. sticticalis. Our findings will provide the basic knowledge for further studies on the molecular mechanisms of the olfactory system of L. sticticalis and potential novel targets for pest control strategies.

Published
2017

29. The Effects of Genetic Variation in FTO rs9939609 on Obesity and Dietary Preferences in Chinese Han Children and Adolescents

Author

Yang, Min, primary, Xu, Yuyang, additional, Liang, Li, additional, Fu, Junfen, additional, Xiong, Feng, additional, Liu, Geli, additional, Gong, Chunxiu, additional, Luo, Feihong, additional, Chen, Shaoke, additional, Xu, Chunxiao, additional, Zhang, Dandan, additional, Li, Zhengli, additional, Zhang, Shuai, additional, Zhang, Yan, additional, Wang, Hao, additional, and Zhu, Yimin, additional

Published
2014
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30. Identification of Serum microRNA Biomarkers for Tuberculosis Using RNA-seq

Author

Zhang, Hongtai, primary, Sun, Zhaogang, additional, Wei, Wenjing, additional, Liu, Zhonghui, additional, Fleming, Joy, additional, Zhang, Shuai, additional, Lin, Nan, additional, Wang, Ming, additional, Chen, Maoshan, additional, Xu, Yuhui, additional, Zhou, Jie, additional, Li, Chuanyou, additional, Bi, Lijun, additional, and Zhou, Guangming, additional

Published
2014
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40. The Effects of Genetic Variation in FTO rs9939609 on Obesity and Dietary Preferences in Chinese Han Children and Adolescents.

Author

Yang, Min, Xu, Yuyang, Liang, Li, Fu, Junfen, Xiong, Feng, Liu, Geli, Gong, Chunxiu, Luo, Feihong, Chen, Shaoke, Xu, Chunxiao, Zhang, Dandan, Li, Zhengli, Zhang, Shuai, Zhang, Yan, Wang, Hao, and Zhu, Yimin

Subjects
HUMAN genetic variation, OBESITY genetics, DIETARY supplements, CHINESE people, DISEASES in teenagers, SINGLE nucleotide polymorphisms, DISEASES
Abstract

The association of the rs9939609 single nucleotide polymorphism in FTO gene with obesity has been extensively investigated in studies of populations of European, African, and Asian ancestry. However, inconsistent results have been reported in Asian populations, and the relationship of FTO variation and dietary behaviors has only rarely been examined in Chinese children and adolescents. The aim of this study was to assess the association of rs9939609 with obesity and dietary preferences in childhood in a Chinese population. Epidemiological data including dietary preferences were collected in interviews using survey questionnaires, and rs9939609 genotype was determined by real-time PCR. The associations of rs9939609 genotypes with obesity and dietary preferences were analyzed by multivariate logistic regression using both additive and dominant models. The results showed that subjects with a TA or AA genotype had an increased risk of obesity compared with the TT participants; the odds ratios (ORs) were 1.47 (95% CI: 1.25–1.71, P = 1.73×10−6), and 3.32 (95% CI: 2.01–5.47, P = 2.68×10−6), respectively. After adjusting for age and gender, body mass index, waist circumference, hip circumference, systolic blood pressure, diastolic blood pressure, fasting blood glucose, triglycerides, and low-density lipoprotein cholesterol were higher, and high-density lipoprotein cholesterol was lower in TA and AA participants than in those with the TT genotype. After additionally controlling for body mass index, the association remained significant only for systolic blood pressure (P = 0.005). Compared with TT participants, those with the AA genotype were more likely to prefer a meat-based diet (OR = 2.81, 95% CI: 1.52–5.21). The combined OR for obesity in participants with TA/AA genotypes and preference for a meat-based diet was 4.04 (95% CI: 2.8–5.81) compared with the TT participants who preferred a plant-based diet. These findings indicate the genetic variation of rs9939609 is associated with obesity and dietary preferences in Chinese children and adolescents. [ABSTRACT FROM AUTHOR]

Published
2014
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41. Ecological Adaption Analysis of the Cotton Aphid (Aphis gossypii) in Different Phenotypes by Transcriptome Comparison.

Author

Li, Zhao-Qun, Zhang, Shuai, Luo, Jun-Yu, Wang, Chun-Yi, Lv, Li-Min, Dong, Shuang-Lin, and Cui, Jin-Jie

Subjects
*COTTON aphid, *PHENOTYPES, *GENETIC transcription, *INSECT pests, *HOST plants, *HEAT shock proteins, *SEEDLINGS, *INSECTS
Abstract

Background: The cotton aphid, Aphis gossypii Glover, is a destructive insect pest worldwide; it directly or indirectly damages (virus transmission) 300 species of host plants. Knowledge of their ecologically adaptive mechanisms at the molecular level may provide an essential and urgent method to effectively control this pest. However, no transcriptome information is available for the cotton aphid and sequence data are scarce. Therefore, we obtained transcriptome data. Results: To facilitate such a study, two cotton aphid transcriptomes at different growth stages of cotton, seedling and summer, were sequenced. A total of 161,396 and 66,668 contigs were obtained and assembled into 83,671 and 42,438 transcripts, respectively. After combining the raw date for both transcriptomes, the sequences were reassembled into 66,695 transcripts, and 52,160 were annotated based on BLASTX analyses. Comparison of the transcriptomes revealed that summer presented less challenges for the cotton aphids than the seedling stage of cotton. In total, 58 putative heat shock protein genes and 66 candidate cytochrome p450 genes were identified with BLASTX. Conclusions: Our results form a basis for exploring the molecular mechanisms of ecological adaption in the cotton aphid. Our study also provides a baseline for the exploration of abiotic stress responses. In addition, it provides large-scale sequence information for further studies on this species. [ABSTRACT FROM AUTHOR]

Published
2013
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42. First Transcriptome and Digital Gene Expression Analysis in Neuroptera with an Emphasis on Chemoreception Genes in Chrysopa pallens (Rambur).

Author

Li, Zhao-Qun, Zhang, Shuai, Ma, Yan, Luo, Jun-Yu, Wang, Chun-Yi, Lv, Li-Min, Dong, Shuang-Lin, and Cui, Jin-Jie

Subjects
*GENE expression, *NEUROPTERA, *CHEMICAL senses, *CHRYSOPA, *NUCLEOTIDE sequence, *PREDATORY animals, *AGRICULTURAL pests
Abstract

Background: Chrysopa pallens (Rambur) are the most important natural enemies and predators of various agricultural pests. Understanding the sophisticated olfactory system in insect antennae is crucial for studying the physiological bases of olfaction and also could lead to effective applications of C. pallens in integrated pest management. However no transcriptome information is available for Neuroptera, and sequence data for C. pallens are scarce, so obtaining more sequence data is a priority for researchers on this species. Results: To facilitate identifying sets of genes involved in olfaction, a normalized transcriptome of C. pallens was sequenced. A total of 104,603 contigs were obtained and assembled into 10,662 clusters and 39,734 singletons; 20,524 were annotated based on BLASTX analyses. A large number of candidate chemosensory genes were identified, including 14 odorant-binding proteins (OBPs), 22 chemosensory proteins (CSPs), 16 ionotropic receptors, 14 odorant receptors, and genes potentially involved in olfactory modulation. To better understand the OBPs, CSPs and cytochrome P450s, phylogenetic trees were constructed. In addition, 10 digital gene expression libraries of different tissues were constructed and gene expression profiles were compared among different tissues in males and females. Conclusions: Our results provide a basis for exploring the mechanisms of chemoreception in C. pallens, as well as other insects. The evolutionary analyses in our study provide new insights into the differentiation and evolution of insect OBPs and CSPs. Our study provided large-scale sequence information for further studies in C. pallens. [ABSTRACT FROM AUTHOR]

Published
2013
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