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Identification of candidate chemosensory genes by transcriptome analysis in Loxostege sticticalis Linnaeus
- Source :
- PLoS ONE, Vol 12, Iss 4, p e0174036 (2017), PLoS ONE
- Publication Year :
- 2017
- Publisher :
- Public Library of Science (PLoS), 2017.
-
Abstract
- Loxostege sticticalis Linnaeus is an economically important agricultural pest, and the larvae cause great damage to crops, especially in Northern China. However, effective and environmentally friendly chemical methods for controlling this pest have not been discovered to date. In the present study, we performed HiSeq2500 sequencing of transcriptomes of the male and female adult antennae, adult legs and third instar larvae, and we identified 54 candidate odorant receptors (ORs), including 1 odorant receptor coreceptor (Orco) and 5 pheromone receptors (PRs), 18 ionotropic receptors (IRs), 13 gustatory receptors (GRs), 34 odorant binding proteins (OBPs), including 1 general odorant binding protein (GOBP1) and 3 pheromone binding proteins (PBPs), 10 chemosensory proteins (CSPs) and 2 sensory neuron membrane proteins (SNMPs). The results of RNA-Seq and RT-qPCR analyses showed the expression levels of most genes in the antennae were higher than that in the legs and larvae. Furthermore, PR4, OR1-4, 7-11, 13-15, 23, 29-32, 34, 41, 43, 47/IR7d.2/GR5b, 45, 7/PBP2-3, GOBP1, OBP3, 8 showed female antennae-biased expression, while PR1/OBP2, 7/IR75d/CSP2 showed male antennae-biased expression. However, IR1, 7d.3, 68a/OBP11, 20-22, 28/CSP9 had larvae enriched expression, and OBP15, 17, 25, 29/CSP5 were mainly expressed in the legs. The results shown above indicated that these genes might play a key role in foraging, seeking mates and host recognition in the L. sticticalis. Our findings will provide the basic knowledge for further studies on the molecular mechanisms of the olfactory system of L. sticticalis and potential novel targets for pest control strategies.
- Subjects :
- 0301 basic medicine
Male
Life Cycles
Sensory Receptors
Odorant binding
Physiology
Social Sciences
lcsh:Medicine
Receptors, Odorant
Biochemistry
Ion Channels
Transcriptome
Larvae
Medicine and Health Sciences
Psychology
Animal Anatomy
Receptor
lcsh:Science
Musculoskeletal System
Phylogeny
Data Management
Genetics
Multidisciplinary
biology
Physics
Phylogenetic Analysis
Genomics
Biological Evolution
Receptors, Pheromone
Lepidoptera
Electrophysiology
Phylogenetics
Larva
Physical Sciences
Pheromone
Insect Proteins
Olfactory Receptors
Legs
Female
Sensory Perception
Anatomy
Research Article
Signal Transduction
Arthropod Antennae
Computer and Information Sciences
Loxostege sticticalis
Biophysics
Neurophysiology
Receptors, Ionotropic Glutamate
Odorant Binding Proteins
03 medical and health sciences
Animals
Animal Physiology
Evolutionary Systematics
Pheromone binding
Gene Prediction
Taxonomy
Evolutionary Biology
Gene Expression Profiling
Limbs (Anatomy)
fungi
lcsh:R
Biology and Life Sciences
Computational Biology
Proteins
Molecular Sequence Annotation
Cell Biology
Ligand-Gated Ion Channels
biology.organism_classification
Genome Analysis
030104 developmental biology
Gene Ontology
Membrane protein
Odorant-binding protein
biology.protein
lcsh:Q
Antennae (Animal Physiology)
Zoology
Neuroscience
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....bd0024cf843e480fb77a8a4dd3b7abb0