1. Iron deposition in autopsied liver on patients receiving long-term TPN
- Author
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Yoshihiro Kawade, Kazuyuki Arakawa, Chie Hishida, Toshie Manabe, Yoshio Hashizume, Hajime Tanaka, Norihiro Ogawa, Takayuki Yamamoto, Takeshi Kanesaka, Hiroyasu Akatsu, Yoshiyuki Masaki, Mitsuo Maruyama, Hirotaka Ohara, Koichi Tsuneyama, and Takayoshi Kanematsu
- Subjects
Male ,Parenteral Nutrition ,Physiology ,Gastroenterology ,Biochemistry ,Negative Staining ,White Blood Cells ,0302 clinical medicine ,Animal Cells ,Medicine and Health Sciences ,Group-Specific Staining ,Infusions, Intravenous ,Aged, 80 and over ,Staining ,Multidisciplinary ,biology ,medicine.diagnostic_test ,Body Fluids ,Chemistry ,medicine.anatomical_structure ,Blood ,Liver ,030220 oncology & carcinogenesis ,Physical Sciences ,Serum iron ,Medicine ,030211 gastroenterology & hepatology ,Female ,Autopsy ,Prussian Blue Staining ,Cellular Types ,Anatomy ,Research Article ,Chemical Elements ,medicine.medical_specialty ,Kupffer Cells ,Science ,Iron ,Immune Cells ,Immunology ,Research and Analysis Methods ,03 medical and health sciences ,Hepcidin ,Internal medicine ,medicine ,Humans ,Vein ,Aged ,Nutrition ,Ferritin ,Blood Cells ,business.industry ,Albumin ,Biology and Life Sciences ,Proteins ,Protein Complexes ,Cell Biology ,Transthyretin ,Parenteral nutrition ,Specimen Preparation and Treatment ,biology.protein ,Hepatocytes ,business - Abstract
BackgroundVitamins and minerals are routinely administered by total parenteral nutrition (TPN). However, in Japan, adjustments in iron dosage are difficult because blended mineral preparations are often used. It is therefore unclear whether the iron content is appropriate in cases of long-term TPN. The aim of the study was to assess the influence of iron administration by long-term TPN on iron deposition in post-mortem liver samples isolated from older deceased patients.MethodsLiver tissues were collected from post-mortem autopsies of 187 patients over a period of 15 years. Samples were stained with Prussian blue and histologically evaluated from Grade 0-V by at least three different observers. Specimens with positive and negative iron staining were compared, and positive samples were grouped according to the level and distribution of the staining. Post-mortem blood obtained from the subclavian vein during autopsy was also analysed. Samples were collected for the measurement of unsaturated serum iron, serum iron, albumin, prealbumin, hepcidin, and IL-6 concentrations.ResultsIron accumulation in the liver was significantly higher in male patients (p = 0.005) with a history of surgery (p = 0.044) or central vein administration of iron (pConclusionsChronic intravenous administration of iron was associated with iron deposition in the liver, even when given the minimum recommended dosage. In long-term TPN patients, the iron dose should therefore be carefully considered.
- Published
- 2020