1. Characterization of Bone Marrow Stromal Abnormalities in a Patient with Constitutional Trisomy 8 Mosaicism and Myelodysplastic Syndrome
- Author
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Lindsay M. Hawkins, Wilma Vanek, Aru Narendran, Hooman Ganjavi, Matthew F.W. Gee, Grant Johnson, Jason W. Barlow, Melvin H. Freedman, and David Malkin
- Subjects
Pathology ,medicine.medical_specialty ,Stromal cell ,Myeloid ,Cell Survival ,medicine.medical_treatment ,Trisomy ,Trisomy 8 ,Bone Marrow ,Cell Line, Tumor ,hemic and lymphatic diseases ,Tumor Cells, Cultured ,Humans ,Medicine ,Progenitor cell ,Child ,Mosaicism ,business.industry ,Hematology ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,Hematopoietic Stem Cells ,medicine.disease ,Coculture Techniques ,Hematopoiesis ,Leukemia ,Haematopoiesis ,Cytokine ,medicine.anatomical_structure ,Oncology ,Myelodysplastic Syndromes ,Pediatrics, Perinatology and Child Health ,Cytokines ,Female ,Bone marrow ,Stromal Cells ,business ,Cell Division ,Chromosomes, Human, Pair 8 - Abstract
The development of myeloid leukemias and myelodysplastic syndrome (MDS) is common in children with trisomy 8 mosaicism. However, the mechanisms by which the presence of an additional chromosome 8 translates to an increased risk of leukemias and MDS is currently unknown. The authors describe the analysis of stromal cells from a pediatric MDS patient with constitutional trisomy 8. Patient and control marrow stromal cells were analyzed for alterations in cytokine production. Clonogenic assays were used to examine stromal support for hematopoiesis. The interplay between leukemia cells and stroma was studied by co-culture experiments. The results indicate that stromal cell function in this patient was seriously altered in favor of progenitor cell proliferation and expansion. This indicates that constitutional trisomy 8 in stromal cells plays a critical role in the pathogenesis of MDS.
- Published
- 2004