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Your search keyword '"Scondotto, S"' showing total 3 results
Start Over Author "Scondotto, S" Journal neurochemical research
3 results on '"Scondotto, S"'

2. Binding of flunitrazepam to differentiating neurons cultured in a chemically defined, hormone-supplemented medium

Author

V. La Bella, R. Guarneri, S Scondotto, Giovanni Savettieri, Giuseppe Salemi, Federico Piccoli, Donatella Ferraro, Savettieri, G., Guarneri, R., Salemi, G., LA BELLA, V., Ferraro, D., Scondotto, S., and Piccoli, F.

Subjects
Central nervous system, Flunitrazepam, Biology, Settore BIO/19 - Microbiologia Generale, Biochemistry, gamma-Aminobutyric acid, GABA, Cellular and Molecular Neuroscience, medicine, Animals, Binding site, Cells, Cultured, gamma-Aminobutyric Acid, Neurons, Fetus, Cell Differentiation, General Medicine, Hormones, In vitro, Culture Media, Cell biology, medicine.anatomical_structure, neuronal culture, Cell culture, Cerebral cortex, Settore MED/26 - Neurologia, Neuroscience, medicine.drug
Abstract

[3H]Flunitrazepam (FNZ) binding to cortical neurons from fetal rat brain was investigated in vitro. The use of a synthetic medium specific for neurons made it possible to plot a developmental curve of3H-FNZ binding in an almost pure neuronal culture. Detectable specific binding was present in vitro at time 0 (that is, the 16th gestational day). A progressive increase of binding, due to an increment in the number of recognition sites, was observed on the subsequent days. The affinity of the specific binding sites to3H-FNZ was enhanced by the addition of exogenous GABA, whereas the density was not affected. © 1990 Plenum Publishing Corporation.

Published
1990

3. Binding of flunitrazepam to differentiating neurons cultured in a chemically defined, hormone-supplemented medium.

Author

Savettieri G, Guarneri R, Salemi G, La Bella V, Ferraro D, Scondotto S, and Piccoli F

Subjects
Animals, Cell Differentiation, Cells, Cultured, Culture Media, Neurons cytology, gamma-Aminobutyric Acid pharmacology, Flunitrazepam metabolism, Hormones pharmacology, Neurons metabolism
Abstract

[3H]Flunitrazepam (FNZ) binding to cortical neurons from fetal rat brain was investigated in vitro. The use of a synthetic medium specific for neurons made it possible to plot a developmental curve of 3H-FNZ binding in an almost pure neuronal culture. Detectable specific binding was present in vitro at time 0 (that is, the 16th gestational day). A progressive increase of binding, due to an increment in the number of recognition sites, was observed on the subsequent days. The affinity of the specific binding sites to 3H-FNZ was enhanced by the addition of exogenous GABA, whereas the density was not affected.

Published
1990
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