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Binding of flunitrazepam to differentiating neurons cultured in a chemically defined, hormone-supplemented medium
- Source :
- Neurochemical Research. 15:773-776
- Publication Year :
- 1990
- Publisher :
- Springer Science and Business Media LLC, 1990.
-
Abstract
- [3H]Flunitrazepam (FNZ) binding to cortical neurons from fetal rat brain was investigated in vitro. The use of a synthetic medium specific for neurons made it possible to plot a developmental curve of3H-FNZ binding in an almost pure neuronal culture. Detectable specific binding was present in vitro at time 0 (that is, the 16th gestational day). A progressive increase of binding, due to an increment in the number of recognition sites, was observed on the subsequent days. The affinity of the specific binding sites to3H-FNZ was enhanced by the addition of exogenous GABA, whereas the density was not affected. © 1990 Plenum Publishing Corporation.
- Subjects :
- Central nervous system
Flunitrazepam
Biology
Settore BIO/19 - Microbiologia Generale
Biochemistry
gamma-Aminobutyric acid
GABA
Cellular and Molecular Neuroscience
medicine
Animals
Binding site
Cells, Cultured
gamma-Aminobutyric Acid
Neurons
Fetus
Cell Differentiation
General Medicine
Hormones
In vitro
Culture Media
Cell biology
medicine.anatomical_structure
neuronal culture
Cell culture
Cerebral cortex
Settore MED/26 - Neurologia
Neuroscience
medicine.drug
Subjects
Details
- ISSN :
- 15736903 and 03643190
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Neurochemical Research
- Accession number :
- edsair.doi.dedup.....b81eebf17c249eb941418d2c864c31b4