1. Identification of β-amyloid species in canine cerebrospinal fluid by mass spectrometry
- Author
-
Ángela González-Martínez, Manuel Sarasa, José Antonio Allué, Leticia Sarasa, and Pedro Pesini
- Subjects
Aging ,Pathology ,medicine.medical_specialty ,Immunoprecipitation ,Biology ,Mass spectrometry ,Mass Spectrometry ,Animal model ,Cerebrospinal fluid ,Dogs ,β amyloid ,Alzheimer Disease ,medicine ,Animals ,Humans ,chemistry.chemical_classification ,Amyloid beta-Peptides ,General Neuroscience ,Molecular biology ,Disease Models, Animal ,Enzyme ,chemistry ,biology.protein ,Neurology (clinical) ,Geriatrics and Gerontology ,Time-of-flight mass spectrometry ,Antibody ,Amyloid Precursor Protein Secretases ,Biomarkers ,Developmental Biology - Abstract
It is well known that several Aβ species, including Aβ40 and Aβ42, are present in cerebrospinal fluid (CSF). Experimental results suggest that these species could play a role in Alzheimer's disease and might also have diagnostic significance. In the present work, the canine CSF β-amyloid species profile has been identified by matrix-assisted laser desorption and ionization–time-of-flight/time of flight mass spectrometry (MALDI-TOF/TOF) analysis after immunoprecipitation with different Aβ-specific antibodies. The results show that species arising from combined β- and γ-secretase activities in humans, such as Aβ1–33, Aβ1–34, Aβ1–37, Aβ1–38, Aβ1–39, Aβ1–40, and Aβ1–42, are also present in dogs. Species arising from combined α- and β-secretase activities, as well as other Aβ-degrading enzymes, are also present in both human and canine CSF, with the exception of Aβ1–13, Aβ1–14, and Aβ1–18, which are not detected in dogs. A large number of species truncated at Glu-3 and Glu-11 have also been detected. To our knowledge, this work describes a most complete Aβ species profile from canine CSF. The similarities between the canine and human CSF Aβ profile reinforce the dog as a highly appropriate animal model for research in Alzheimer's disease.
- Published
- 2012