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Identification of β-amyloid species in canine cerebrospinal fluid by mass spectrometry
- Source :
- Neurobiology of aging. 34(9)
- Publication Year :
- 2012
-
Abstract
- It is well known that several Aβ species, including Aβ40 and Aβ42, are present in cerebrospinal fluid (CSF). Experimental results suggest that these species could play a role in Alzheimer's disease and might also have diagnostic significance. In the present work, the canine CSF β-amyloid species profile has been identified by matrix-assisted laser desorption and ionization–time-of-flight/time of flight mass spectrometry (MALDI-TOF/TOF) analysis after immunoprecipitation with different Aβ-specific antibodies. The results show that species arising from combined β- and γ-secretase activities in humans, such as Aβ1–33, Aβ1–34, Aβ1–37, Aβ1–38, Aβ1–39, Aβ1–40, and Aβ1–42, are also present in dogs. Species arising from combined α- and β-secretase activities, as well as other Aβ-degrading enzymes, are also present in both human and canine CSF, with the exception of Aβ1–13, Aβ1–14, and Aβ1–18, which are not detected in dogs. A large number of species truncated at Glu-3 and Glu-11 have also been detected. To our knowledge, this work describes a most complete Aβ species profile from canine CSF. The similarities between the canine and human CSF Aβ profile reinforce the dog as a highly appropriate animal model for research in Alzheimer's disease.
- Subjects :
- Aging
Pathology
medicine.medical_specialty
Immunoprecipitation
Biology
Mass spectrometry
Mass Spectrometry
Animal model
Cerebrospinal fluid
Dogs
β amyloid
Alzheimer Disease
medicine
Animals
Humans
chemistry.chemical_classification
Amyloid beta-Peptides
General Neuroscience
Molecular biology
Disease Models, Animal
Enzyme
chemistry
biology.protein
Neurology (clinical)
Geriatrics and Gerontology
Time-of-flight mass spectrometry
Antibody
Amyloid Precursor Protein Secretases
Biomarkers
Developmental Biology
Subjects
Details
- ISSN :
- 15581497
- Volume :
- 34
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Neurobiology of aging
- Accession number :
- edsair.doi.dedup.....2c8d27db7b66a17027867e38d137f940