1. Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells
- Author
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Laura Conti, Roberto Ruiu, Diego Alberti, S. Geninatti Crich, Stefania Lanzardo, Silvio Aime, Juan Carlos Cutrin, Federica Cavallo, and M. Cadenazzi
- Subjects
Curcumin ,Theranostic Nanomedicine ,MRI contrast agent ,Anti-Inflammatory Agents ,Contrast Media ,Antineoplastic Agents ,Breast Neoplasms ,Receptors, Cell Surface ,INGENIERÍAS Y TECNOLOGÍAS ,chemistry.chemical_compound ,Therapeutic index ,BREAST CANCER ,Cell Line, Tumor ,Iron-Binding Proteins ,Animals ,Humans ,THERANOSTIC ,General Materials Science ,Otras Nanotecnología ,Horses ,skin and connective tissue diseases ,Receptor ,Cell Proliferation ,Nanotecnología ,Drug Carriers ,biology ,Temperature ,Scavenger Receptors, Class A ,Magnetic Resonance Imaging ,Ferritin ,Agar ,chemistry ,Cell culture ,Apoferritins ,Ferritins ,Immunology ,Cancer cell ,MCF-7 Cells ,Cancer research ,biology.protein ,Female ,Materials Science (all) ,FERRITIN ,Spleen ,MRI - Abstract
In this work the selective uptake of native horse spleen ferritin and apoferritin loaded with MRI contrast agents has been assessed in human breast cancer cells (MCF-7 and MDA-MB-231). The higher expression of L-ferritin receptors (SCARA5) led to an enhanced uptake in MCF-7 as shown in T2 and T1 weighted MR images, respectively. The high efficiency of ferritin internalization in MCF-7 has been exploited for the simultaneous delivery of curcumin, a natural therapeutic molecule endowed with antineoplastic and anti-inflammatory action, and the MRI contrast agent Gd-HPDO3A. This theranostic system is able to treat selectively breast cancer cells over-expressing ferritin receptors. By entrapping in apoferritin both Gd-HPDO3A and curcumin, it was possible to deliver a therapeutic dose of 167 μg ml−1 (as calculated by MRI) of this natural drug to MCF-7 cells, thus obtaining a significant reduction of cell proliferation. Fil: Geninatti Crich, S.. Universitã â Di Torino; Italia Fil: Cadenazzi, M.. Universitã â Di Torino; Italia Fil: Lanzardo, S.. Universitã â Di Torino; Italia Fil: Conti, L.. Universitã â Di Torino; Italia Fil: Ruiu, R.. Universitã â Di Torino; Italia Fil: Alberti, D.. Universitã â Di Torino; Italia Fil: Cavallo, F.. Universitã â Di Torino; Italia Fil: Cutrin, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina. Università  Di Torino; Italia Fil: Aime, S.. Universitã â Di Torino; Italia
- Published
- 2015
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