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Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells

Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells

Authors :
Laura Conti
Roberto Ruiu
Diego Alberti
S. Geninatti Crich
Stefania Lanzardo
Silvio Aime
Juan Carlos Cutrin
Federica Cavallo
M. Cadenazzi
Source :
Nanoscale. 7:6527-6533
Publication Year :
2015
Publisher :
Royal Society of Chemistry (RSC), 2015.

Abstract

In this work the selective uptake of native horse spleen ferritin and apoferritin loaded with MRI contrast agents has been assessed in human breast cancer cells (MCF-7 and MDA-MB-231). The higher expression of L-ferritin receptors (SCARA5) led to an enhanced uptake in MCF-7 as shown in T2 and T1 weighted MR images, respectively. The high efficiency of ferritin internalization in MCF-7 has been exploited for the simultaneous delivery of curcumin, a natural therapeutic molecule endowed with antineoplastic and anti-inflammatory action, and the MRI contrast agent Gd-HPDO3A. This theranostic system is able to treat selectively breast cancer cells over-expressing ferritin receptors. By entrapping in apoferritin both Gd-HPDO3A and curcumin, it was possible to deliver a therapeutic dose of 167 μg ml−1 (as calculated by MRI) of this natural drug to MCF-7 cells, thus obtaining a significant reduction of cell proliferation. Fil: Geninatti Crich, S.. Universitã â Di Torino; Italia Fil: Cadenazzi, M.. Universitã â Di Torino; Italia Fil: Lanzardo, S.. Universitã â Di Torino; Italia Fil: Conti, L.. Universitã â Di Torino; Italia Fil: Ruiu, R.. Universitã â Di Torino; Italia Fil: Alberti, D.. Universitã â Di Torino; Italia Fil: Cavallo, F.. Universitã â Di Torino; Italia Fil: Cutrin, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina. Università  Di Torino; Italia Fil: Aime, S.. Universitã â Di Torino; Italia

Details

ISSN :
20403372 and 20403364
Volume :
7
Database :
OpenAIRE
Journal :
Nanoscale
Accession number :
edsair.doi.dedup.....e37f2162a294df157802bc657e6f7b68
Full Text :
https://doi.org/10.1039/c5nr00352k