1. Differential expression of insulin-like growth factor binding proteins in murine hematopoietic stromal cell lines
- Author
-
Teresa F. Clawson, Wei Hua Lee, and Mervin C. Yoder
- Subjects
medicine.medical_specialty ,Mesoderm ,Stromal cell ,medicine.medical_treatment ,Radioimmunoassay ,Biology ,Biochemistry ,Polymerase Chain Reaction ,Mice ,Endocrinology ,Insulin-Like Growth Factor II ,Internal medicine ,medicine ,Animals ,Yolk sac ,Insulin-Like Growth Factor I ,Molecular Biology ,Cells, Cultured ,Growth factor ,Cell biology ,Blot ,Insulin-Like Growth Factor Binding Proteins ,medicine.anatomical_structure ,Cell culture ,Culture Media, Conditioned ,embryonic structures ,Bone marrow ,Endoderm ,Stromal Cells ,Interleukin-1 - Abstract
The expression of the insulin-like growth factor (IGF) system in the hematopoietic microenvironment during ontogeny was studied utilizing immortalized murine cell lines established from several sites of hematopoiesis. Conditioned media was obtained from tissue cultures of murine yolk sac endoderm and mesoderm, fetal liver and adult bone marrow stromal cell lines. IGF-I and -II were quantified by radioimmunoassay and RT-PCR. The presence of insulin-like growth factor binding proteins (IGFBPs) in conditioned media was determined by Western ligand blot and Western immunoblot. IGF-I and IGF-II were present in conditioned media from every stromal cell line and differential expression of the IGFBPs was found among the hematopoietic sites. Stimulation of the cell lines with interleukin-1 alpha altered the IGFBPs in yolk sac endoderm and bone marrow conditioned media. We report that IGF-I and -II are expressed in stromal cell lines obtained from different ontogenic sites of hematopoiesis and IGFBPs are differentially expressed by these sites. The expression of IGFBPs, but not IGFs, is in part regulated by interleukin-1 alpha.
- Published
- 1996