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Differential expression of insulin-like growth factor binding proteins in murine hematopoietic stromal cell lines
Differential expression of insulin-like growth factor binding proteins in murine hematopoietic stromal cell lines
- Source :
- Molecular and cellular endocrinology. 120(1)
- Publication Year :
- 1996
-
Abstract
- The expression of the insulin-like growth factor (IGF) system in the hematopoietic microenvironment during ontogeny was studied utilizing immortalized murine cell lines established from several sites of hematopoiesis. Conditioned media was obtained from tissue cultures of murine yolk sac endoderm and mesoderm, fetal liver and adult bone marrow stromal cell lines. IGF-I and -II were quantified by radioimmunoassay and RT-PCR. The presence of insulin-like growth factor binding proteins (IGFBPs) in conditioned media was determined by Western ligand blot and Western immunoblot. IGF-I and IGF-II were present in conditioned media from every stromal cell line and differential expression of the IGFBPs was found among the hematopoietic sites. Stimulation of the cell lines with interleukin-1 alpha altered the IGFBPs in yolk sac endoderm and bone marrow conditioned media. We report that IGF-I and -II are expressed in stromal cell lines obtained from different ontogenic sites of hematopoiesis and IGFBPs are differentially expressed by these sites. The expression of IGFBPs, but not IGFs, is in part regulated by interleukin-1 alpha.
- Subjects :
- medicine.medical_specialty
Mesoderm
Stromal cell
medicine.medical_treatment
Radioimmunoassay
Biology
Biochemistry
Polymerase Chain Reaction
Mice
Endocrinology
Insulin-Like Growth Factor II
Internal medicine
medicine
Animals
Yolk sac
Insulin-Like Growth Factor I
Molecular Biology
Cells, Cultured
Growth factor
Cell biology
Blot
Insulin-Like Growth Factor Binding Proteins
medicine.anatomical_structure
Cell culture
Culture Media, Conditioned
embryonic structures
Bone marrow
Endoderm
Stromal Cells
Interleukin-1
Subjects
Details
- ISSN :
- 03037207
- Volume :
- 120
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Molecular and cellular endocrinology
- Accession number :
- edsair.doi.dedup.....3894dba8c659441b26698b6c3796de8c