1. Incorporation of 3H-Thymidine by Different Prokaryotic Groups in Relation to Temperature and Nutrients in a Lacustrine Ecosystem
- Author
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Aurélie Thénot, Didier Debroas, M. Richardot, Delphine Boucher, Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Centre National de la Recherche Scientifique (CNRS)-Université d'Auvergne - Clermont-Ferrand I (UdA), and Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Cytophagaceae ,Uptake ,Cytophaga ,In situ ,Molecular hybridization ,Nutrient ,MESH: Biomass ,MESH: Ecosystem ,Biomass ,MESH: In Situ Hybridization, Fluorescence ,Food science ,MESH: Phosphorus ,MESH: Nitrogen ,Incubation ,In Situ Hybridization, Fluorescence ,Soil Microbiology ,Phylogeny ,2. Zero hunger ,0303 health sciences ,Ecology ,biology ,Temperature ,Bacteriosis ,Phosphorus ,Adaptation, Physiological ,Nitrogen ,MESH: Temperature ,6. Clean water ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Infection ,Soil Science ,chemistry.chemical_element ,MESH: Carbon ,Tritium ,Microbiology ,03 medical and health sciences ,Microbial ecology ,Proteobacteria ,Cytophagales ,Microbial community ,Ecosystem ,Archaeobacteria ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,Bacteria ,MESH: Cytophaga ,030306 microbiology ,MESH: Proteobacteria ,biology.organism_classification ,MESH: Adaptation, Physiological ,Carbon ,Treatment ,Eubacteria ,MESH: Bacteria ,MESH: Soil Microbiology ,MESH: Tritium ,chemistry ,Microbial population biology ,Thymidine ,MESH: Thymidine ,Archaea - Abstract
International audience; The incorporation of [3H-methyl] thymidine (3H-TdR) by Eubacteria, bacterial groups (alpha- and beta-Proteobacteria, Cytophaga-Flavobacter), and Archaea was measured according to temperature (7 and 17 degrees C) and nutrient levels (nitrogen, phosphorus, and carbon) in a lacustrine system (Sep, France). Short-term incubation was performed using a combination of microautoradiography and fluorescent in situ hybridization. Irrespective of the temperatures and nutrients studied, all the major phylogenetic bacterial groups assimilated 3H-TdR, and in most of the treatments studied, the proportion of beta-Proteobacteria taking up 3H-TdR was higher than those in the other bacterial groups. The proportion of Bacteria and different bacterial groups studied incorporating 3H-TdR were significantly increased, approximately 1.5-fold, by temperature except for alpha-Proteobacteria (7.6-fold). The nutrient effect was not the same for the different bacterial groups according to the temperatures studied. The proportions of alpha-Proteobacteria (at both temperatures) and Cytophaga-Flavobacter (at 7 degrees C) taking up 3H-TdR were significantly decreased and increased by adding N and P, respectively. Also, adding N, P, and C increased and decreased the percentage of beta-Proteobacteria incorporating 3H-TdR at 7 and 17 degrees C, respectively. The archaeal community showed a similar proportion of active cells (i.e., 3H-TdR) to the bacterial community, and uptake of 3H-TdR by Archaea was significantly increased (P < 0.05) by both temperature and nutrients. Thus, the assimilation of 3H-TdR by bacterial groups and Archaea in lacustrine system is significantly controlled by both temperature and nutrients.
- Published
- 2006