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Incorporation of 3H-Thymidine by Different Prokaryotic Groups in Relation to Temperature and Nutrients in a Lacustrine Ecosystem
- Source :
- Microbial Ecology, Microbial Ecology, Springer Verlag, 2006, 52 (3), pp.399-407. ⟨10.1007/s00248-006-9074-4⟩, Microbial ecology, Microbial ecology, 2006, 52 (3), pp.399-407. ⟨10.1007/s00248-006-9074-4⟩
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- International audience; The incorporation of [3H-methyl] thymidine (3H-TdR) by Eubacteria, bacterial groups (alpha- and beta-Proteobacteria, Cytophaga-Flavobacter), and Archaea was measured according to temperature (7 and 17 degrees C) and nutrient levels (nitrogen, phosphorus, and carbon) in a lacustrine system (Sep, France). Short-term incubation was performed using a combination of microautoradiography and fluorescent in situ hybridization. Irrespective of the temperatures and nutrients studied, all the major phylogenetic bacterial groups assimilated 3H-TdR, and in most of the treatments studied, the proportion of beta-Proteobacteria taking up 3H-TdR was higher than those in the other bacterial groups. The proportion of Bacteria and different bacterial groups studied incorporating 3H-TdR were significantly increased, approximately 1.5-fold, by temperature except for alpha-Proteobacteria (7.6-fold). The nutrient effect was not the same for the different bacterial groups according to the temperatures studied. The proportions of alpha-Proteobacteria (at both temperatures) and Cytophaga-Flavobacter (at 7 degrees C) taking up 3H-TdR were significantly decreased and increased by adding N and P, respectively. Also, adding N, P, and C increased and decreased the percentage of beta-Proteobacteria incorporating 3H-TdR at 7 and 17 degrees C, respectively. The archaeal community showed a similar proportion of active cells (i.e., 3H-TdR) to the bacterial community, and uptake of 3H-TdR by Archaea was significantly increased (P < 0.05) by both temperature and nutrients. Thus, the assimilation of 3H-TdR by bacterial groups and Archaea in lacustrine system is significantly controlled by both temperature and nutrients.
- Subjects :
- Cytophagaceae
Uptake
Cytophaga
In situ
Molecular hybridization
Nutrient
MESH: Biomass
MESH: Ecosystem
Biomass
MESH: In Situ Hybridization, Fluorescence
Food science
MESH: Phosphorus
MESH: Nitrogen
Incubation
In Situ Hybridization, Fluorescence
Soil Microbiology
Phylogeny
2. Zero hunger
0303 health sciences
Ecology
biology
Temperature
Bacteriosis
Phosphorus
Adaptation, Physiological
Nitrogen
MESH: Temperature
6. Clean water
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Infection
Soil Science
chemistry.chemical_element
MESH: Carbon
Tritium
Microbiology
03 medical and health sciences
Microbial ecology
Proteobacteria
Cytophagales
Microbial community
Ecosystem
Archaeobacteria
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
Bacteria
MESH: Cytophaga
030306 microbiology
MESH: Proteobacteria
biology.organism_classification
MESH: Adaptation, Physiological
Carbon
Treatment
Eubacteria
MESH: Bacteria
MESH: Soil Microbiology
MESH: Tritium
chemistry
Microbial population biology
Thymidine
MESH: Thymidine
Archaea
Subjects
Details
- ISSN :
- 1432184X and 00953628
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Microbial Ecology
- Accession number :
- edsair.doi.dedup.....60aad612fb131cfeaceccbd483c882bb