1. Differentiation of mouse embryonic stem cells into endothelial cells: genetic selection and potential use in vivo
- Author
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Gimond , Clotilde, Marchetti , Sandrine, Pagès , Gilles, Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Institut de signalisation, biologie du développement et cancer ( ISBDC ), Université Nice Sophia Antipolis ( UNS ), and Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS )
- Subjects
MESH: Cell Differentiation ,MESH : Cell Culture Techniques ,Cell Transplantation ,Green Fluorescent Proteins ,Cell Culture Techniques ,Mice, Nude ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,[ SDV.BBM.BM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,MESH: Stem Cells ,MESH: Extracellular Matrix ,MESH : Green Fluorescent Proteins ,[ SDV.CAN ] Life Sciences [q-bio]/Cancer ,Mice ,MESH: Green Fluorescent Proteins ,MESH: Reverse Transcriptase Polymerase Chain Reaction ,MESH : Mice ,MESH : Cells, Cultured ,MESH: Mice, Nude ,MESH : Embryo, Mammalian ,Animals ,Humans ,MESH: Animals ,MESH: Endothelial Cells ,MESH: Mice ,Cells, Cultured ,MESH: Cell Culture Techniques ,MESH: Humans ,MESH : Endothelial Cells ,Reverse Transcriptase Polymerase Chain Reaction ,Stem Cells ,MESH : Humans ,MESH : Reverse Transcriptase Polymerase Chain Reaction ,MESH : Extracellular Matrix ,MESH: Embryo, Mammalian ,MESH : Mice, Nude ,Endothelial Cells ,MESH: Antigens, CD31 ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Cell Differentiation ,Embryo, Mammalian ,MESH : Cell Transplantation ,Extracellular Matrix ,Platelet Endothelial Cell Adhesion Molecule-1 ,MESH: Cell Transplantation ,MESH : Antigens, CD31 ,MESH : Stem Cells ,MESH : Animals ,MESH : Cell Differentiation ,MESH: Cells, Cultured - Abstract
International audience; Embryonic stem (ES) cells are pluripotent cells derived from blastocyst-stage embryos. They are characterized by their infinite self-renewal capacity and their ability to differentiate into many cell types in vitro as well as in vivo. The present protocol describes culture conditions that allow efficient differentiation of mouse ES cells toward the endothelial lineage, both in two-dimensional cultures and in three-dimensional, multicellular embryoid bodies. We also provide a protocol for establishing recombinant ES cell clones, giving the example of cells expressing green fluorescent protein and an antibiotic resistance gene under the control of an endothelial-specific promoter. These transgenes allow the visualization and the genetic selection of endothelial cells from a heterogeneous population of differentiating ES cells. Potential applications for these differentiation models are given, including the study of the endothelial cell lineage and its derivatives, the testing of molecules involved in angiogenesis, and the potential use of selected endothelial cells in vivo.
- Published
- 2006