Back to Search
Start Over
Differentiation of mouse embryonic stem cells into endothelial cells: genetic selection and potential use in vivo
- Source :
- Methods in Molecular Biology, Methods in Molecular Biology, Humana Press/Springer Imprint, 2006, 330, pp.303-29. ⟨10.1385/1-59745-036-7:303⟩, Methods in Molecular Biology (Clifton then Totova), Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2006, 330, pp.303-29. 〈10.1385/1-59745-036-7:303〉
- Publication Year :
- 2006
-
Abstract
- International audience; Embryonic stem (ES) cells are pluripotent cells derived from blastocyst-stage embryos. They are characterized by their infinite self-renewal capacity and their ability to differentiate into many cell types in vitro as well as in vivo. The present protocol describes culture conditions that allow efficient differentiation of mouse ES cells toward the endothelial lineage, both in two-dimensional cultures and in three-dimensional, multicellular embryoid bodies. We also provide a protocol for establishing recombinant ES cell clones, giving the example of cells expressing green fluorescent protein and an antibiotic resistance gene under the control of an endothelial-specific promoter. These transgenes allow the visualization and the genetic selection of endothelial cells from a heterogeneous population of differentiating ES cells. Potential applications for these differentiation models are given, including the study of the endothelial cell lineage and its derivatives, the testing of molecules involved in angiogenesis, and the potential use of selected endothelial cells in vivo.
- Subjects :
- MESH: Cell Differentiation
MESH : Cell Culture Techniques
Cell Transplantation
Green Fluorescent Proteins
Cell Culture Techniques
Mice, Nude
[SDV.CAN]Life Sciences [q-bio]/Cancer
[ SDV.BBM.BM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
MESH: Stem Cells
MESH: Extracellular Matrix
MESH : Green Fluorescent Proteins
[ SDV.CAN ] Life Sciences [q-bio]/Cancer
Mice
MESH: Green Fluorescent Proteins
MESH: Reverse Transcriptase Polymerase Chain Reaction
MESH : Mice
MESH : Cells, Cultured
MESH: Mice, Nude
MESH : Embryo, Mammalian
Animals
Humans
MESH: Animals
MESH: Endothelial Cells
MESH: Mice
Cells, Cultured
MESH: Cell Culture Techniques
MESH: Humans
MESH : Endothelial Cells
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells
MESH : Humans
MESH : Reverse Transcriptase Polymerase Chain Reaction
MESH : Extracellular Matrix
MESH: Embryo, Mammalian
MESH : Mice, Nude
Endothelial Cells
MESH: Antigens, CD31
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Cell Differentiation
Embryo, Mammalian
MESH : Cell Transplantation
Extracellular Matrix
Platelet Endothelial Cell Adhesion Molecule-1
MESH: Cell Transplantation
MESH : Antigens, CD31
MESH : Stem Cells
MESH : Animals
MESH : Cell Differentiation
MESH: Cells, Cultured
Subjects
Details
- ISSN :
- 10643745 and 19406029
- Volume :
- 330
- Database :
- OpenAIRE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Accession number :
- edsair.pmid.dedup....d53c3e199037f729eb143950421b824e