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A study was conducted on the photochemical solid-phase phosphoramidite DNA synthesis technique. The findings indicate that the coupling efficiency of these amidites in the synthesis of homopolymers was in the range of 80-97%, with purines having lower efficiency than pyrimidines. Moreover, the results suggest that light-directed in situ synthesis of short oligonucleotides will be an effective approach in preparing hybridization surfaces for detection of complementary sequences.

An inverse 5'-3' photochemical phosphotriester DNA synthesis makes use of a photoremovable phosphate protecting group. The group is generated by fixing the 5'-hydroxyl of the DNA to a solid support thus making available the phosphate protecting group to the 3'-phosphate in the phosphotriester coupling method. The 3'-phosphodiester fused to the base is activated at the same time allowing the reaction between a 3'-activated phosphate and a 5'-hydroxyl. The method is able to synthesize short oligonucleotides which are attached to the support via their 5'-terminals.