1. Extracellular Histones Promote Pulmonary Fibrosis in Patients With Coal Workers' Pneumoconiosis.
- Author
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Yanlin Zhang, Li Guan, Yimu Zheng, Lijun Mao, Shuqiang Li, and Jinyuan Zhao
- Subjects
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PULMONARY fibrosis , *CELL proliferation , *AUTOANTIBODIES , *EXTRACELLULAR space , *FIBROBLASTS , *HISTONES , *DUST diseases , *TRANSFORMING growth factors-beta , *SEVERITY of illness index , *IN vitro studies , *BLOOD , *PREVENTION - Abstract
Objective: This investigation assessed the profibrotic role that extracellular histones play in the pathogenesis of coal workers' pneumoconiosis (CWP). Methods: The correlation of extracellular histones with small opacity profusion (SOP) and transforming growth factor-ß (TGF-ß) was analyzed. The stimulating effect of extracellular histones on pulmonary fibroblast was assessed in vitro. Results: The levels of extracellular histones in plasma were positively correlated with SOP and TGF-ß in the coal miners investigated. Plasma collected from patients with CWP caused apparent lung fibroblast proliferation, while anti-H4 antibody antagonized the stimulating effect of the patient plasma by blocking histone H4. In vitro experiments showed that extracellular histones directly stimulated fibroblast proliferation. Conclusion: Consistent with our hypothesis, the concentrations of extracellular histones were indices of the severity of pulmonary fibrosis in simple CWP, and extracellular histones-targeted Intervention could inhibit the proliferation of lung fibroblast. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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