Search

Your search keyword '"Y, Shimoji"' showing total 4 results
Start Over Author "Y, Shimoji" Journal journal of microbiological methods
4 results on '"Y, Shimoji"'

1. Differentiation of Salmonella vaccinated and infected animals by serological detection of antibody to T3SS effector SsaK in an indirect ELISA.

Author

Aribam SD, Nakayama M, Ichimura S, Tokuyama K, Hara Y, Ogawa Y, Shimoji Y, and Eguchi M

Subjects
Animals, Enzyme-Linked Immunosorbent Assay, Serologic Tests, Antibodies, Bacterial, Salmonella, Salmonella Infections diagnosis
Abstract

The differentiation of animals that are vaccinated and those that are naturally infected with Salmonella is difficult by conventional serological tests. We have shown here an indirect Enzyme-linked immunosorbent assay for detection of Salmonella infection based on the presence of a Type III secretory effector SsaK in the sera., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Published
2023
Full Text
View/download PDF

2. Development of an SNP-based PCR assay for rapid differentiation of a Japanese live vaccine strain from field isolates of Erysipelothrix rhusiopathiae.

Author

Shiraiwa K, Ogawa Y, Eguchi M, Hikono H, Kusumoto M, and Shimoji Y

Subjects
Animals, DNA, Bacterial genetics, Polymorphism, Single Nucleotide genetics, Sequence Analysis, DNA, Bacterial Vaccines genetics, Erysipelothrix classification, Erysipelothrix genetics, Erysipelothrix Infections microbiology, Molecular Typing methods, Polymerase Chain Reaction methods
Abstract

The differentiation of vaccine from non-vaccine isolates is important for disease control. Based on single nucleotide polymorphisms identified by comparison of the genomes of the Koganei 65-0.15 vaccine strain and a reference strain of Erysipelothrix rhusiopathiae, we developed a PCR assay that can differentiate the vaccine strain from field isolates., (Copyright © 2015 Elsevier B.V. All rights reserved.)

Published
2015
Full Text
View/download PDF

3. Monoclonal antibody-based competitive enzyme-linked immunosorbent assay to detect antibodies to O:4 Salmonella in the sera of livestock and poultry.

Author

Aribam SD, Ogawa Y, Matsui H, Hirota J, Okamura M, Akiba M, Shimoji Y, and Eguchi M

Subjects
Animals, Cattle, Chickens, Enzyme-Linked Immunosorbent Assay instrumentation, Horses, Livestock blood, Poultry Diseases microbiology, Salmonella immunology, Salmonella Infections, Animal microbiology, Swine, Antibodies, Bacterial analysis, Antibodies, Monoclonal analysis, Enzyme-Linked Immunosorbent Assay methods, Poultry Diseases blood, Salmonella isolation & purification, Salmonella Infections, Animal blood
Abstract

Serotyping is an important element for surveillance of Salmonella. In this study, an anti-O:4 Salmonella monoclonal antibody-based competitive enzyme-linked immunosorbent assay that could identify Salmonella infection in cow, pig, horse, and chicken was developed. This detection system can therefore be useful for a wide range of animals and for humans., (Copyright © 2014 Elsevier B.V. All rights reserved.)

Published
2015
Full Text
View/download PDF

4. A rapid differentiation method for enteroinvasive Escherichia coli.

Author

Aribam SD, Hirota J, Kusumoto M, Harada T, Shiraiwa K, Ogawa Y, Shimoji Y, and Eguchi M

Subjects
Cell Line, Humans, Biological Assay methods, Escherichia coli growth & development, Escherichia coli Infections diagnosis
Abstract

Enteroinvasive Escherichia coli (EIEC) comprise 21 major serotypes defined by the presence of O and H antigens, and diagnosis depends on determining its invasive potential. Using HEp-2 cells infected with an EIEC strain, we developed a simple growth-dependent assay that differentiated EIEC strain from non-invasive strains 6 h after infection., (Copyright © 2014 Elsevier B.V. All rights reserved.)

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results