Development of an SNP-based PCR assay for rapid differentiation of a Japanese live vaccine strain from field isolates of Erysipelothrix rhusiopathiae.

Authors :: Shiraiwa K
Ogawa Y
Eguchi M
Hikono H
Kusumoto M
Shimoji Y
Source :: Journal of microbiological methods [J Microbiol Methods] 2015 Oct; Vol. 117, pp. 11-3. Date of Electronic Publication: 2015 Jul 04.
Publication Year :: 2015
Abstract: The differentiation of vaccine from non-vaccine isolates is important for disease control. Based on single nucleotide polymorphisms identified by comparison of the genomes of the Koganei 65-0.15 vaccine strain and a reference strain of Erysipelothrix rhusiopathiae, we developed a PCR assay that can differentiate the vaccine strain from field isolates.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)

Subjects :: Animals
DNA, Bacterial genetics
Polymorphism, Single Nucleotide genetics
Sequence Analysis, DNA
Bacterial Vaccines genetics
Erysipelothrix classification
Erysipelothrix genetics
Erysipelothrix Infections microbiology
Molecular Typing methods
Polymerase Chain Reaction methods

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