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Objective　To observe whether Huashi Runjing Decoction can inhibit the inflammatory response in rats with desmoplastic male dry eye disease, and to explore its potential mechanism of action. Methods　60 healthy male SD rats were randomly divided into 4 groups：sham surgery group of 15, negative control group of 15, positive control group of 15, Huashi Running Decoction group of 15) . The sham operated group rats only underwent abdominal incision without removing both testicles and epididymis；Rats in the negative control group, positive control group and Huashi Runjing Decoction group were removed both testicles and epididymis. After 1 week of modeling, the model was validated by fluorescein staining (FL), break-up time (BUT), and tear secretion test (SIT) . After the wound healed, the rats were given the appropriate drug interventions. The degree of corneal breakage, tear film rupture time and tear secretion of rats in each group were detected by FL method, BUT method and SIT method respectively after 8 weeks of drug administration. The levels of COX-2, TNF-α and IL-6 in the serum of each group of rats were observed by ELISA; HE staining was used to observe the pathomorphological changes in the lacrimal gland tissue of rats in each group. The expression of key inflammatory factors COX-2, TNF-α and IL-6 in the lacrimal gland tissues of rats in each group was observed by immunohistochemistry and Western blot. Results　Compared with the sham-operated group, the negative control group, positive control group and Huashi Runjing Decoction group showed an increase in the area of sodium fluorescein positive staining in the cornea, a significant increase in FL score, a shortening of tear film rupture time and a decrease in tear secretion, an increase in serum COX-2, TNF-α and IL-6 levels, atrophy of the lacrimal gland tissue vesicles, an irregular shape, a disorganized structure, a decrease in intracytoplasmic zymogen particles, and a large number of inflammatory cell infiltrations. The expression levels of inflammatory factors COX-2, TNF-α and IL-6 increased in the tissues ( P < 0.05) ; Compared with the negative control group, the Huashi Runjing Decoction group showed a significant decrease in the area of sodium fluorescein positive staining, a significant decrease in FL score, a significant increase in tear film rupture time and tear secretion, a decrease in serum COX-2, TNF-α and IL-6 levels, a decrease in atrophy of the lacrimal gland tissue vesicles, a more regular shape, a clearer structure, an increase in the number of intracytoplasmic zymogen particles, an increase in inflammatory cell infiltration and a decrease in the area of lacrimal gland tissue. The expression levels of COX-2, TNF-α and IL-6 in lacrimal gland tissues were reduced (P < 0.05) . Conclusion　Huashi Runjing Decoction could reduce the protein expression of COX-2, TNF-α and IL-6 in the lacrimal gland tissue of castrated male dry eye disease rats, and inhibit the inflammatory response. [ABSTRACT FROM AUTHOR]

Objective　 To analyze the clinical features of adult hemophagocytic syndrome （HPS） and the correlation between the levels of various cytokines and prognosis. Methods　 The data of 19 adult patients with hemophagocytic syndrome were collected, and the clinical features and laboratory tests were analyzed. The blood samples were collected for 12 cytokine levels detection, and the correlation between the levels and prognosis was analyzed. Results　The levels of cytokines IL-1β, IL-2, IL-4, IL-8, IL-10, IL-6, IFN-γand TNF-α in HPS patients were higher than those in non-HPS non-infected patients （P < 0.05）. The levels of IL-4, IL-5, IL10, IFN-γ and TNF-α in HPS patients were higher than those in non-HPS patients （P < 0.05）, the ROC curve of IL-10, IFN-γ and TNF-α showed that the AUC of IL-10, IFN-γ and TNF-α was between 0.7 and 0.9, indicating accuracy in the diagnosis of HPS. Fib was significantly lower than that in the survival group （P < 0.05）, while there were no significant differences in age, sex, clinical features （fever, liver, spleen, lymphadenopathy） and laboratory tests （ALT, AST, TBIL, ALB, Fer, TG, ACN, HGB, PLT） between the HPS death group and the survival group （P > 0.05）, and the levels of IL-1β, IL-8 and TNF-α in HPS death group were significantly higher than those in survival group （P < 0.05）. Conclusion　HPS may present increased levels of various cytokines, among which IL-10, IFN-γ and TNF-α may be significant for the diagnosis of HPS. The significant decrease of Fib and the significant increase of IL-1β, IL-8 and TNF-α may indicate a poor prognosis. [ABSTRACT FROM AUTHOR]

Objective　To study the efficacy and safety of rivaroxaban after stent implantation of the femoral popliteal atherosclerosis occlusion stent. Methods　Eighty-seven patients who met the criteria were randomly divided into 45 cases in the treatment group and 42 cases in the control group. There was no statistically significant difference in the general information between the two groups. The treatment group was given rivaroxaban tablets 10 mg each time， po， qd and aspirin enteric-coated tablets 100 mg each time， po， qd； the control group was given aspirin enteric-coated tablets 100 mg， po， qd each time， both groups were given Atorvastatin 10 mg， po， qd and Beraprost sodium tablets 40 μg， po， tid， all patients were urged to quit smoking. Observe the relevant efficacy indicators such as ABI， claudication distance， stent restenosis， stent mural thrombosis， and secondary intervention rate of the two groups of patients on the day of postoperative day，3 months and 6 months after treatment， and the safety indicators such as adverse drug reactions， major adverse cardiac and cerebrovascular events， minor and major bleeding， the results were analyzed statistically. Results　At 6 months， there were differences in the stent mural thrombosis， ankle brachial index and claudication distance between the two groups （P < 0.05）， no significant difference in in-stent restenosis （P > 0.05），There was no statistically significant difference in the logrank test of the second intervention rate between the two groups （P > 0.05），and there was no statistical difference in safety comparison between the two groups（ P > 0.05） . Conclusion　Rivaroxaban combined with aspirin can significantly reduce stent mural thrombosis and delay the decline in postoperative ABI and claudication distance， which may benefit the long-term prognosis of patients. [ABSTRACT FROM AUTHOR]

Objective To compare the effect of three oral care methods (oral scrubbing, oral rinsing and oral scrubbing and rinsing combined) on postoperative about oral cancer. Methods 150 patients who had oral cancer surgery in our department from July 2017 to January 2020 were randomly divided into 3 groups, and given three kinds of oral care methods respectively. The differences of oral cleanliness, comfort, wound healing and oral infection among the three groups were observed. Results After 7 days of oral care by combining oral scrubbing with rinsing, the patients' oral cleanliness, wound healing and oral infection were better than those in the other two groups, and the patients in the oral rinsing group had the best oral comfort. The differences were statistically significant (P < 0.05). Conclusion A combination of oral scrubbing with rinsing is one of the most effective oral care methods to treat patients with oral cancer after operation [ABSTRACT FROM AUTHOR]

Objective To investigate the role of DNMT3A in IDH1 mutation induced acute myeloidleukemia genome methylation . Methods To study the effect of DNMT3A expression level on IDH1 induced genomic hypermethylation, human AML cell lines HL60 and THP-1 were transfected with IDH1，DNMT3A mutant expression plasmid. Results （1） The levels of 2-HG and 5-methylcytosine （5-mC） were increased in the primary AML cells with IDH1 mutation and the human AML cell lines HL60 or THP-1 transfected with IDH1 mutant expression plasmid was increased，while the level of DNMT3A mRNA and its protease activity also increased（P<0.05）.（2） Inhibiting the expression of DN MT3Ain HL60 or THP-1 cells transfected with IDH1 mutant expression plasmid could reduce the content of 5-mC（ P< 0.05），but the expression level of 2-HG was not affected （P = 0.989）.（3）Compared with HL60 cells transfected with IDH1 mutation （IDH1 R132H） expression plasmids alone， the expression plasmids transfected IDH1 mutation （IDH1 R132H） and DNMT3A mutation （DNMT3A R882H） decreased the content of 5-mC in cells（ P< 0.05）， but the expression of 2-HG wasn't affected （p=1.294） . Conclusion DNMT3A participates in the genomic hypermethylation induced by IDH1 mutation. [ABSTRACT FROM AUTHOR]

Objective To study the effects of methamphetamine on the expression of 5- Hydroxytryptophan (5-HT)， interleukin-6 (IL-6) and nuclear fact kappa B (NF-κB) in rats' hippocampus. Methods Thirty SD rats were randomly allocated into normal control group，METH1d group，METH3d group， METH5d group and METH7d group，with 6 rats in each group．Intraperitoneal injections of METH [15mg/(kg·time)] were conducted at 9:00am and 21:00pm every day．After injection，scoring was performed in combination with observation on their stereotyped behavior. We established different models for rats suffering from 1-day，3-day，5-day，and 7-day METH acute poisoning. After 24 hours of the last intraperitoneal injections of METH，hippocampal tissues were isolated．Enzyme linked immunoorbent assay (ELISA) was applied to detect the expression level of 5-HT and IL-6 in rats hippocampus. Western blot was used to detect the expression level of NF-κBp65．Results Compared with the control group，the expression of 5-HT was reduced in different METH groups，and the decrease was statistically significant in METH5d and METH7d groups (with P<0.05 and P<0.01 respectively) . Compared with the control group，the expression of IL-6 was increased in different METH groups， and the increase was statistically significant in METH5d and METH7d groups (with P<0.05 and P<0.01 respectively) . Compared with the control group， the expression of NF-κB was increased in different groups， and the increase was statistically significant only in METH7d group (P<0.01) . Conclusion METH administration could result in a significant reduce of 5-HT expression and increases of IL-6 and NF-κB expressions in rats hippocampus. [ABSTRACT FROM AUTHOR]

Objective To study the effect of methamphetamine on the expression of DA，5-HTand TNF-α in rats hippocampus. Methods (1) The in vivo toxicity model was established by injecting MA 15 mg/(kg.time)， two times a day for 5 days. The in vitro toxicity model was established by incubating MA with mixed hippocampal neurons/microglia for 24 hours. (2) ELISA was used to detect the content of DA， 5-HT and TNF-α in rat hippocampus and cell culture supernatant. Results On the fifth day，Methamphetamine treated rats showed hair errecting， weight loss， rapid head shaking，circling or head dorsal movement， with a stereotyped score of 5. The levels of DA and 5-HT decreased， and the content of TNF-alpha elevated in rats hippocampus of MA group . The levels of DA ， 5-HT decreased and the content of TNF- alpha increased in cell cultural supernatants of MA group 24 hours. Conclusion The changes of DA， 5-HT and TNF- alpha are involved in rat hippocampal damage induced by methamphetamine. [ABSTRACT FROM AUTHOR]

Objective Analysis of the establishment of a set of operational performance appraisal program， observation and analysis of its application to strengthening the management of nursing. Methods With key indicators， questionnaire survey and expert consultation， performance appraisal implementation plan was established， and the satisfaction degree of nursing staff and of doctors with nursing staff before and after the implementation plan were compared. Results After the implementation of the program，the satisfaction scores of the nursing staff in the evaluation of merit，promotion，scheduling，performance distribution，retention intention and comprehensive satisfaction were significantly improved （ P＜0.05） . At the same time，after the implementation of the program，the satisfaction score of doctors was significantly improved in nursing staff's emergency response ability，communication，responsibility，implementation of medical treatment and comprehensive satisfaction （PP <0.05） . Comprehensive satisfaction degree of nursing staff with themselves and of doctors with the nursing staff has improved from82.18%and 83.55%before implementation to 97.02%and 99.05%after implementation. Conclusion Implementation of nursing performance evaluation program standardized nursing management， and thus improved the management level， and promoted nursing quality. As doctors' and patients' satisfaction with nursing staff improved， nursing staff greatly improved their working initiative，enthusiasm and creativity. It created a better incentive mechanismto make full use of human resources. [ABSTRACT FROM AUTHOR]

Objective To study the effect of methamphetamine on the expression of TNF-α，IL-βand CD22 in rats hippocampus. Method MA （15 mg/kg）was daily administrated （I.P）twice a day for 1day，3days and 5days and normal control group， one-day group， three-day group and five-day group were established. After injection of MA， the stereotyped behavior activities in rats were scored. ELISA method was applied to detect the changes of TNF alpha and IL -1 beta in experimental rats. Western blot was used to detect the expression of CD22. Results Compared with normal control group， the expression of TNF alpha and IL -1beta increased in different MA treated groups， and there was a significant difference in 5-day group（P < 0.01）；the expression of CD22 increased spirally and there was a significant difference in 5 days group（P < 0.01） . Conclusion MA administration resulted in significant increase in TNF alpha，IL -1beta and CD22 expression in rats hippocampus. [ABSTRACT FROM AUTHOR]