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Previously, we observed that serum from humans immune to dengue serotype 1 (dengue-1) neutralized the American genotype of dengue serotype 2 (American-2) to a greater ex- tent than it neutralized the Asian genotype of dengue serotype 2 (Asian-2). To determine if this activity is protective, Aotus nancymac monkeys were infected with dengue-1 followed by either American-2 or Asian-2. Dengue- 1-infected animals produced antibody with neutralizing titers of 2656 antibodies against dengue-1, 409 against American-2, and <20 against Asian-2. Infection with American-2 did not produce detectable viremia in either dengue-1-immune or den- gue-1-naive animals. These findings support the hypothesis that dengue- 1 immunity might have prevented disease or altered the severity of disease in individuals sequentially infected with dengue-1 and American-2. [ABSTRACT FROM AUTHOR]

BALB/c mice were experimentally infected with murine cytomegalovirus (MCMV) to discover whether latent MCMV persisted in aging mice and to examine the effect of aging on MCMV reactivation. Latently infected mice received saline, cyclophosphamide, or allogeneic blood at 6 and 18 months of age. MCMV DNA was detected by polymerase chain reaction in submaxillary salivary gland biopsy specimens from saline-treated young and old mice. Evidence ofMCMV reactivation was sought byculture of biopsy specimens and byMCMV IgG ELISA of pre- and posttreatment sera from all animals. Veryfewcyclophosphamide-or saline-treated mice reactivated MCMV at either age, but young transfused mice reactivated MCMV significantly more often than did old transfused mice. These experiments indicate that MCMV tiNA persists in the salivary gland of aging mice but that the likelihood of MCMV reactivation does not increase with age. [ABSTRACT FROM PUBLISHER]