1. Identification of two Thormählen-positive compounds from melanotic urine by gas chromatography-mass spectrometry
- Author
-
J Duchon, S Pavel, G T Nagel, Z. Schwippelová, and Frits A. J. Muskiet
- Subjects
Chromatography ,Indoles ,Trimethylsilyl ,Elution ,Chemistry ,Liver Neoplasms ,General Chemistry ,Urine ,Mass spectrometry ,Gas Chromatography-Mass Spectrometry ,Hydrolysis ,chemistry.chemical_compound ,Humans ,Indicators and Reagents ,Gas chromatography ,Chromatography, Thin Layer ,Gas chromatography–mass spectrometry ,Neoplasm Metastasis ,Glucuronide ,Melanoma - Abstract
The isolation of two Thormahlen-positive compounds from the urine of a patient with malignant melanoma and the elucidation of their structure by gas chromatography—mass spectrometry is described. The compounds were isolated using a poly-N-vinylpyrrolidone column and separated by preparative thin-layer chromatography. After elution they were analyzed by gas chromatography and gas chromatography—mass spectrometry as their trimethylsilyl derivatives and after hydrolysis also as their tert.-butyldimethylsilyl derivatives. The results showed the main Thormahlen-positive compound A to be the glucuronide of 5-hydroxy-6-methoxyindole, whereas the minor compound AX appeared to be the glucuronide of its isomer 6-hydroxy-5-methoxyindole.
- Published
- 1981