1. In vitro drusen model: three-dimensional spheroid culture of retinal pigment epithelial cells
- Author
-
Wolfram Eichler, Johannes Kacza, Andreas Bringmann, Soichiro Kuwayama, Yousef Yafai, Aki Kato, Hideaki Usui, Akiko Nishiwaki, Ayae Kubota, Yuichiro Ogura, Kazuki Ohashi, Peter Wiedemann, Noriaki Takase, Rina Wako, Tsutomu Yasukawa, Johannes Seeger, and Lanors Landiev
- Subjects
0301 basic medicine ,Retinal pigment epithelium ,genetic structures ,Spheroid ,Retinal ,Cell Biology ,Drusen ,Macular degeneration ,Biology ,medicine.disease ,eye diseases ,Lipofuscin ,Cell biology ,Pathogenesis ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,chemistry ,030221 ophthalmology & optometry ,Extracellular ,medicine ,sense organs - Abstract
Age-related macular degeneration (AMD) is a leading cause of legal blindness in people over 50 years of age in many developed countries. Drusen are yellowish extracellular deposits beneath retinal pigment epithelium (RPE) found in aging eyes and considered as a biomarker of AMD. However, the biogenesis of drusen has not been elucidated. We reported previously that multicellular spheroids comprised of human RPE cells constructed a well-differentiated monolayer of RPE with Bruch's membrane. We determined that RPE spheroids exhibited drusen formation between RPE and Bruch's membrane with expression of many drusen-associated proteins such as amyloid ß and complement components, the expression of which was altered by a challenge with oxidative stress. Artificial lipofuscin-loaded RPE spheroids yielded drusen more frequently. In the current study, we showed that drusen originates from the RPE. This culture system is an attractive tool for use as an in vitro drusen model, which might help elucidate the biogenesis of drusen and the pathogenesis of related diseases such as AMD.
- Published
- 2018