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In vitro drusen model: three-dimensional spheroid culture of retinal pigment epithelial cells
- Source :
- Journal of Cell Science.
- Publication Year :
- 2018
- Publisher :
- The Company of Biologists, 2018.
-
Abstract
- Age-related macular degeneration (AMD) is a leading cause of legal blindness in people over 50 years of age in many developed countries. Drusen are yellowish extracellular deposits beneath retinal pigment epithelium (RPE) found in aging eyes and considered as a biomarker of AMD. However, the biogenesis of drusen has not been elucidated. We reported previously that multicellular spheroids comprised of human RPE cells constructed a well-differentiated monolayer of RPE with Bruch's membrane. We determined that RPE spheroids exhibited drusen formation between RPE and Bruch's membrane with expression of many drusen-associated proteins such as amyloid ß and complement components, the expression of which was altered by a challenge with oxidative stress. Artificial lipofuscin-loaded RPE spheroids yielded drusen more frequently. In the current study, we showed that drusen originates from the RPE. This culture system is an attractive tool for use as an in vitro drusen model, which might help elucidate the biogenesis of drusen and the pathogenesis of related diseases such as AMD.
- Subjects :
- 0301 basic medicine
Retinal pigment epithelium
genetic structures
Spheroid
Retinal
Cell Biology
Drusen
Macular degeneration
Biology
medicine.disease
eye diseases
Lipofuscin
Cell biology
Pathogenesis
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
chemistry
030221 ophthalmology & optometry
Extracellular
medicine
sense organs
Subjects
Details
- ISSN :
- 14779137 and 00219533
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Science
- Accession number :
- edsair.doi...........78f7d3d0d380680decf9005041d0fd51