1. Diffuse fluorescence fiber probe for in vivo detection of circulating cells
- Author
-
Mark Niedre, Judith Runnels, Charles P. Lin, Xuefei Tan, Vivian Pera, and Neha Sardesai
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,Diffuse reflectance infrared fourier transform ,Biomedical Engineering ,Cancer metastasis ,Mice, Nude ,Cell Count ,01 natural sciences ,010309 optics ,Biomaterials ,03 medical and health sciences ,Mice ,Circulating tumor cell ,In vivo ,Neoplasms ,0103 physical sciences ,medicine ,Animals ,Neoplasm Metastasis ,Fluorescent Dyes ,Chemistry ,Phantoms, Imaging ,Neoplastic Cells, Circulating ,Fluorescence ,Atomic and Molecular Physics, and Optics ,In vitro ,Electronic, Optical and Magnetic Materials ,030104 developmental biology ,Fiber probe ,Preclinical imaging ,Biomedical engineering ,Research Papers: Sensing - Abstract
There has been significant recent interest in the development of technologies for enumeration of rare circulating cells directly in the bloodstream in many areas of research, for example, in small animal models of circulating tumor cell dissemination during cancer metastasis. We describe a fiber-based optical probe that allows fluorescence detection of labeled circulating cells in vivo in a diffuse reflectance configuration. We validated this probe in a tissue-mimicking flow phantom model in vitro and in nude mice injected with fluorescently labeled multiple myeloma cells in vivo. Compared to our previous work, this design yields an improvement in detection signal-to-noise ratio of 10 dB, virtually eliminates problematic motion artifacts due to mouse breathing, and potentially allows operation in larger animals and limbs.
- Published
- 2017