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Selective targeting of the retinal pigment epithelium using an acousto-optic laser scanner
- Source :
- Journal of Biomedical Optics. 10:064014
- Publication Year :
- 2005
- Publisher :
- SPIE-Intl Soc Optical Eng, 2005.
-
Abstract
- Selective targeting of the retinal pigment epithelium (RPE) is a new strategy for treating certain retinal disorders while preserving adjacent photoreceptors. The treatment currently relies on a complex laser system to produce the required microsecond pulse structure. In our new approach, we scan the focus of a continuous-wave (cw) laser beam with acousto-optic deflectors to produce microsecond-long exposures at each RPE cell. Experiments were performed in vitro with a bench-top scanner on samples of young bovine RPE and in vivo on Dutch belted rabbits with a slit-lamp adapted scanner. Effective dose 50% (ED50) for RPE damage was determined in vitro by fluorescence cell viability assay and in vivo by fluorescein angiography. Damage to individual RPE cells was achieved with laser power on the order of 100 mW. Using separated scan lines, we demonstrate selectivity in the form of alternating lines of dead and surviving cells that resemble the scan pattern. Selectivity is also shown by the absence of retinal thermal coagulation in vivo. Selective RPE damage is feasible by rapidly scanning a cw laser beam. The scanning device is an attractive alternative to conventional laser coagulation and pulsed laser targeting of the RPE.
- Subjects :
- Materials science
Retinal Disorder
Laser scanning
Cell Survival
Biomedical Engineering
In Vitro Techniques
Sensitivity and Specificity
law.invention
Biomaterials
chemistry.chemical_compound
Optics
law
In vivo
medicine
Animals
Laser power scaling
Pigment Epithelium of Eye
Cells, Cultured
Retina
Laser Coagulation
Retinal pigment epithelium
business.industry
Epithelial Cells
Retinal
Acoustics
Equipment Design
Laser
eye diseases
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Equipment Failure Analysis
Treatment Outcome
medicine.anatomical_structure
chemistry
Cattle
Rabbits
sense organs
business
Biomedical engineering
Subjects
Details
- ISSN :
- 10833668
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....d4190f7944c2114da91441324bc8292c