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Your search keyword '"Majcherczyk, Paul"' showing total 3 results
Start Over Author "Majcherczyk, Paul" Journal journal of bacteriology
3 results on '"Majcherczyk, Paul"'

1. Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite 1-2-amino-4-methoxy-trans-3-butenoic acid

Author

Lee, Xiaoyun, Fox, Aine, Sufrin, Janice, Henry, Hugues, Majcherczyk, Paul, Haas, Dieter, and Reimmann, Cornelia

Subjects
Pseudomonas aeruginosa -- Genetic aspects, Gene expression -- Physiological aspects, Antimetabolites -- Properties, Carboxylic acids -- Properties, Biological sciences
Abstract

L.2-Amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction. doi: 10.1128/JB.00492-10

Published
2010

3. Identification of the Biosynthetic Gene Cluster for the Pseudomonas aeruginosa Antimetabolite L-2-Amino-4-Methoxy-trans-3-Butenoic Acid.

Author

Xiaoyun Lee, Fox, Áine, Sufrin, Janice, Henry, Hugues, Majcherczyk, Paul, Haas, Dieter, and Reimmann, Cornelia

Subjects
*ANTIMETABOLITES, *PSEUDOMONAS aeruginosa, *ANTIBIOTICS, *BIOSYNTHESIS, *ENZYME inhibitors
Abstract

L-2-Amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction. [ABSTRACT FROM AUTHOR]

Published
2010
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