Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite 1-2-amino-4-methoxy-trans-3-butenoic acid

L.2-Amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction. doi: 10.1128/JB.00492-10