1. Photosynthetic CO 2 Conversion to Fatty Acid Ethyl Esters (FAEEs) Using Engineered Cyanobacteria.
- Author
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Lee HJ, Choi J, Lee SM, Um Y, Sim SJ, Kim Y, and Woo HM
- Subjects
- Acetyl Coenzyme A metabolism, Aldehyde-Lyases metabolism, Esters chemistry, Esters metabolism, Ethanol metabolism, Fatty Acids chemistry, Photosynthesis, Synechococcus genetics, Carbon Dioxide metabolism, Fatty Acids metabolism, Metabolic Engineering methods, Synechococcus metabolism
- Abstract
Metabolic engineering of cyanobacteria has received attention as a sustainable strategy to convert carbon dioxide to fatty acid-derived chemicals that are widely used in the food and chemical industries. Herein, Synechococcus elongatus PCC 7942, a model cyanobacterium, was engineered for the first time to produce fatty acid ethyl esters (FAEEs) from CO
2 . Due to the lack of an endogenous ethanol production pathway and wax ester synthase (AftA) activity in the wild-type cyanobacterium, we metabolically engineered S. elongatus PCC 7942 by expressing heterologous AftA and introducing the ethanol pathway, resulting in detectable peaks of FAEEs. To enhance FAEE production, a heterologous phosphoketolase pathway was introduced in the FAEE-producing strain to supply acetyl-CoA. Subsequent optimization of the cyanobacterial culture with a hexadecane overlay resulted in engineered S. elongatus PCC 7942 that produced photosynthetic FAEEs (10.0 ± 0.7 mg/L/OD730 ) from CO2 . This paper is the first report of photosynthetic production of FAEEs from CO2 in cyanobacteria.- Published
- 2017
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