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Photosynthetic CO 2 Conversion to Fatty Acid Ethyl Esters (FAEEs) Using Engineered Cyanobacteria.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2017 Feb 15; Vol. 65 (6), pp. 1087-1092. Date of Electronic Publication: 2017 Feb 02. - Publication Year :
- 2017
-
Abstract
- Metabolic engineering of cyanobacteria has received attention as a sustainable strategy to convert carbon dioxide to fatty acid-derived chemicals that are widely used in the food and chemical industries. Herein, Synechococcus elongatus PCC 7942, a model cyanobacterium, was engineered for the first time to produce fatty acid ethyl esters (FAEEs) from CO <subscript>2</subscript> . Due to the lack of an endogenous ethanol production pathway and wax ester synthase (AftA) activity in the wild-type cyanobacterium, we metabolically engineered S. elongatus PCC 7942 by expressing heterologous AftA and introducing the ethanol pathway, resulting in detectable peaks of FAEEs. To enhance FAEE production, a heterologous phosphoketolase pathway was introduced in the FAEE-producing strain to supply acetyl-CoA. Subsequent optimization of the cyanobacterial culture with a hexadecane overlay resulted in engineered S. elongatus PCC 7942 that produced photosynthetic FAEEs (10.0 ± 0.7 mg/L/OD <subscript>730</subscript> ) from CO <subscript>2</subscript> . This paper is the first report of photosynthetic production of FAEEs from CO <subscript>2</subscript> in cyanobacteria.
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 65
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 28128561
- Full Text :
- https://doi.org/10.1021/acs.jafc.7b00002