1. Structural characterization and immunomodulatory activity of an arabinogalactan from Jasminum sambac (L.) Aiton tea processing waste.
- Author
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Huang, Hai, Yang, Xiang, Li, Wei, Han, Qifeng, Xu, Zhizhen, Xia, Wei, Wu, Mengqi, and Zhang, Wenqing
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ARABINOGALACTAN , *TUMOR necrosis factors , *JASMINE , *MOLECULAR weights , *SEPHAROSE - Abstract
An arabinogalactan named JSP-1a was isolated from Jasmine tea processing waste by DEAE Sepharose FF and Sephacryl S-200 HR chromatography. Polysaccharide JSP-1a, with an average molecular weight of 87.5 kDa, was composed of galactose (59.60 %), arabinose (33.89 %), mannose (4.81 %), and rhamnose (1.70 %). JSP-1a was found to be a type II arabinogalactan comprising the main backbone of 1, 6-linked Gal p residues, and the side chain containing α-T-Ara f , α-1,5-Ara f , β-T-Gal p , β-1,3-Gal p , and β-1,4-Man p residues was attached to the O-3 position of β-1,3,6-Gal p residues. Evidence from bioactivity assays indicated that JSP-1a possessed potent immunomodulatory effects on RAW264.7 macrophages: treatment with JSP-1a increased phagocytosis, activated NF-κB p65 translocation, and promoted the production of NO, reactive oxygen species (ROS), the tumor necrosis factor (TNF)-α, and interleukin (IL)-6. Furthermore, inhibition of Toll-like receptor 4 caused the suppression of NO release and cytokines secretion, which indicated that TLR-4/NF-κB pathway might play a significant role in JSP-1a-induced macrophages' immune response. The results of this study could provide a theoretical basis of JSP-1a as a safe immunostimulatory functional foods or a treatment for immunological diseases. [Display omitted] [ABSTRACT FROM AUTHOR]
- Published
- 2023
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