Back to Search
Start Over
Structural characterization and immunomodulatory activity of an arabinogalactan from Jasminum sambac (L.) Aiton tea processing waste.
- Source :
-
International Journal of Biological Macromolecules . Apr2023, Vol. 235, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- An arabinogalactan named JSP-1a was isolated from Jasmine tea processing waste by DEAE Sepharose FF and Sephacryl S-200 HR chromatography. Polysaccharide JSP-1a, with an average molecular weight of 87.5 kDa, was composed of galactose (59.60 %), arabinose (33.89 %), mannose (4.81 %), and rhamnose (1.70 %). JSP-1a was found to be a type II arabinogalactan comprising the main backbone of 1, 6-linked Gal p residues, and the side chain containing α-T-Ara f , α-1,5-Ara f , β-T-Gal p , β-1,3-Gal p , and β-1,4-Man p residues was attached to the O-3 position of β-1,3,6-Gal p residues. Evidence from bioactivity assays indicated that JSP-1a possessed potent immunomodulatory effects on RAW264.7 macrophages: treatment with JSP-1a increased phagocytosis, activated NF-κB p65 translocation, and promoted the production of NO, reactive oxygen species (ROS), the tumor necrosis factor (TNF)-α, and interleukin (IL)-6. Furthermore, inhibition of Toll-like receptor 4 caused the suppression of NO release and cytokines secretion, which indicated that TLR-4/NF-κB pathway might play a significant role in JSP-1a-induced macrophages' immune response. The results of this study could provide a theoretical basis of JSP-1a as a safe immunostimulatory functional foods or a treatment for immunological diseases. [Display omitted] [ABSTRACT FROM AUTHOR]
- Subjects :
- *ARABINOGALACTAN
*TUMOR necrosis factors
*JASMINE
*MOLECULAR weights
*SEPHAROSE
Subjects
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 235
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 162891359
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2023.123816