1. Structural features, anti-coagulant and anti-adhesive potentials of blue crab (Portunus segnis) chitosan derivatives: Study of the effects of acetylation degree and molecular weight
- Author
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Suming Li, Jalel Gargouri, Marwa Hamdi, Ikram Ben Amor, Moncef Nasri, Rim Nasri, École Nationale d'Ingénieurs de Sfax | National School of Engineers of Sfax (ENIS), Université de Sfax - University of Sfax, Université de Monastir - University of Monastir (UM), Institut Européen des membranes (IEM), and Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)
- Subjects
Brachyura ,[CHIM.THER]Chemical Sciences/Medicinal Chemistry ,02 engineering and technology ,Cellulase ,Thrombin time ,Biochemistry ,Chitosan ,03 medical and health sciences ,chemistry.chemical_compound ,Structural Biology ,Adhesives ,medicine ,Animals ,Humans ,Blue crab chitosan derivatives ,Thermal stability ,Blood Coagulation ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,medicine.diagnostic_test ,biology ,Thrombin ,Acetylation ,General Medicine ,Adhesion ,021001 nanoscience & nanotechnology ,Molecular Weight ,[CHIM.POLY]Chemical Sciences/Polymers ,chemistry ,Biofilms ,biology.protein ,Biophysics ,Degradation (geology) ,Antiadhesive ,anticoagulant ,0210 nano-technology ,Partial thromboplastin time - Abstract
International audience; The present study was undertaken to establish a distinct relationship between blue crab chitosan (Cs) acetylation degree (AD) and molecular weight (Mw) and its structural features, thermal properties and bioactivity. Therefore, chitosans with different AD were prepared and Cellulase was used to produce Cs derivatives with decreasing Mw. Results clearly display a decrease of the ordered structure of Cs, with the increase of AD and the decrease of Mw. Thermal stability/degradation screening disclose a greater thermal resistance for Cs with lower AD and higher Mw. The anti-adhesive potential of Cs was, additionally, studied, as function of AD and Mw. The effectiveness of Cs in preventing biofilm adhesion was strongly influenced by its AD and Mw, with the lowest inhibition values for higher AD and lower Mw. Interestingly, the effectiveness of Cs in disrupting pre-formed biofilms increased with decreasing Mw. Moreover, Cs derivatives were found to be advantageously efficient in prolonging human blood clotting times, based on data of activated partial thromboplastin time, Quick time and thrombin time assays, typically for the intrinsic coagulation pathway. Accordingly, depending on the predicted application of Cs, either in food, biomedical and pharmaceutical industries, AD and Mw are critical traits to be inevitably reflected on.
- Published
- 2020