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Your search keyword '"Ryutaro Fukumura"' showing total 2 results
Start Over "Ryutaro Fukumura" Topic genetics Journal immunogenetics
2 results on '"Ryutaro Fukumura"'

1. Identification of four highly conserved regions in DNA-PKcs

Author

Masumi Abe, Ryoko Araki, Hirokazu Takahashi, Ryutaro Fukumura, Tatsuya Ohhata, Kouich Tatsumi, Akira Kawahara, and Akira Fujimori

Subjects
DNA, Complementary, Immunology, MAPK7, Molecular Sequence Data, Cell Cycle Proteins, DNA-Activated Protein Kinase, Protein Serine-Threonine Kinases, MAP3K7, MAP3K8, Conserved sequence, Cell Line, Mice, Xenopus laevis, Catalytic Domain, Genetics, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, Conserved Sequence, Genomic organization, biology, Base Sequence, Sequence Homology, Amino Acid, Cyclin-dependent kinase 2, Nuclear Proteins, Minichromosome Maintenance Complex Component 4, DNA-Binding Proteins, enzymes and coenzymes (carbohydrates), biology.protein, Cyclin-dependent kinase 9, biological phenomena, cell phenomena, and immunity, Cyclin-dependent kinase 7, Chickens
Abstract

The gene for the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is responsible for severe combined immune deficiency (SCID) and its products associate with Ku70/Ku86 autoantigens, c-Abl, and other factors to exert its roles. Investigations to date suggest that DNA-PKcs comprises several regions which specifically interact with these known and other as yet unidentified factors. Nevertheless, the relationship between the structure and function of the DNA-PKcs molecule is poorly understood. Here we report cloning of the entire DNA-PKcs cDNA from chicken and Xenopus laevis. Comparative study of the DNA-PKcs polypeptides from four different vertebrates revealed at least three novel conserved regions in addition to the carboxyl-terminal region containing the phosphatidylinositol-3 kinase domain. We also demonstrated that the four vertebrates share the common genomic organization between a licensing factor, MCM4, and DNA-PKcs, both of which locate in a head-to-head manner within a few kilobase intervals. These data provide useful clues for the further genetic study of this huge multifunctional enzyme.

Published
2000

2. Identification of four highly conserved regions in DNA-PKcs.

Author

Fujimori, Akira, Araki, Ryoko, Fukumura, Ryutaro, Ohhata, Tatsuya, Takahashi, Hirokazu, Kawahara, Akira, Tatsumi, Kouich, and Abe, Masumi

Subjects
GENETICS, PROTEIN kinases, DNA, IMMUNODEFICIENCY, ANTIGENS, GENOMICS
Abstract

The gene for the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is responsible for severe combined immune deficiency (SCID) and its products associate with Ku70/Ku86 autoantigens, c-Abl, and other factors to exert its roles. Investigations to date suggest that DNA-PKcs comprises several regions which specifically interact with these known and other as yet unidentified factors. Nevertheless, the relationship between the structure and function of the DNA-PKcs molecule is poorly understood. Here we report cloning of the entire DNA-PKcs cDNA from chicken and Xenopus laevis. Comparative study of the DNA-PKcs polypeptides from four different vertebrates revealed at least three novel conserved regions in addition to the carboxyl-terminal region containing the phosphatidylinositol-3 kinase domain. We also demonstrated that the four vertebrates share the common genomic organization between a licensing factor, MCM4, and DNA-PKcs, both of which locate in a head-to-head manner within a few kilobase intervals. These data provide useful clues for the further genetic study of this huge multifunctional enzyme. [ABSTRACT FROM AUTHOR]

Published
2000
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