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Identification of four highly conserved regions in DNA-PKcs
- Source :
- Immunogenetics. 51(11)
- Publication Year :
- 2000
-
Abstract
- The gene for the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is responsible for severe combined immune deficiency (SCID) and its products associate with Ku70/Ku86 autoantigens, c-Abl, and other factors to exert its roles. Investigations to date suggest that DNA-PKcs comprises several regions which specifically interact with these known and other as yet unidentified factors. Nevertheless, the relationship between the structure and function of the DNA-PKcs molecule is poorly understood. Here we report cloning of the entire DNA-PKcs cDNA from chicken and Xenopus laevis. Comparative study of the DNA-PKcs polypeptides from four different vertebrates revealed at least three novel conserved regions in addition to the carboxyl-terminal region containing the phosphatidylinositol-3 kinase domain. We also demonstrated that the four vertebrates share the common genomic organization between a licensing factor, MCM4, and DNA-PKcs, both of which locate in a head-to-head manner within a few kilobase intervals. These data provide useful clues for the further genetic study of this huge multifunctional enzyme.
- Subjects :
- DNA, Complementary
Immunology
MAPK7
Molecular Sequence Data
Cell Cycle Proteins
DNA-Activated Protein Kinase
Protein Serine-Threonine Kinases
MAP3K7
MAP3K8
Conserved sequence
Cell Line
Mice
Xenopus laevis
Catalytic Domain
Genetics
Animals
Humans
Amino Acid Sequence
Cloning, Molecular
Conserved Sequence
Genomic organization
biology
Base Sequence
Sequence Homology, Amino Acid
Cyclin-dependent kinase 2
Nuclear Proteins
Minichromosome Maintenance Complex Component 4
DNA-Binding Proteins
enzymes and coenzymes (carbohydrates)
biology.protein
Cyclin-dependent kinase 9
biological phenomena, cell phenomena, and immunity
Cyclin-dependent kinase 7
Chickens
Subjects
Details
- ISSN :
- 00937711
- Volume :
- 51
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Immunogenetics
- Accession number :
- edsair.doi.dedup.....0e4ab3eafe9e85de026d5b7e169107f3