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Start Over Author "Rongying Ou" Journal genome biology
2 results on '"Rongying Ou"'

1. Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology

Author

Xiao Chen, Mi Liu, Hongyan Lou, Yiyi Lu, Meng-Tao Zhou, Rongying Ou, Yunsheng Xu, and Kai-Fu Tang

Subjects
TRIM21, Trim-away, Protein degradation, Knockdown, Biology (General), QH301-705.5, Genetics, QH426-470
Abstract

Abstract Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embryos. Trim-Away depletes proteins faster than morpholinos, which enables analysis of protein function during early embryogenesis. Furthermore, Trim-Away can be applied to evaluate the role of maternally contributed proteins in zebrafish embryos. Our findings indicate that Trim-Away is a powerful tool to perform functional analysis of proteins during zebrafish development.

Published
2019
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2. Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology

Author

Rongying Ou, Xiao Chen, Yiyi Lu, Kai-Fu Tang, Mi Liu, Meng-Tao Zhou, Yunsheng Xu, and Hongyan Lou

Subjects
Cell type, Embryo, Nonmammalian, animal structures, Morpholino, lcsh:QH426-470, ved/biology.organism_classification_rank.species, Short Report, Endogeny, Biology, Protein degradation, Animals, Genetically Modified, 03 medical and health sciences, 0302 clinical medicine, Animals, Model organism, Zebrafish, lcsh:QH301-705.5, 030304 developmental biology, 0303 health sciences, Gene knockdown, ved/biology, fungi, biology.organism_classification, Phenotype, Cell biology, lcsh:Genetics, Ribonucleoproteins, lcsh:Biology (General), Trim-away, Proteolysis, embryonic structures, Knockdown, 030217 neurology & neurosurgery, TRIM21, Biotechnology
Abstract

Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embryos. Trim-Away depletes proteins faster than morpholinos, which enables analysis of protein function during early embryogenesis. Furthermore, Trim-Away can be applied to evaluate the role of maternally contributed proteins in zebrafish embryos. Our findings indicate that Trim-Away is a powerful tool to perform functional analysis of proteins during zebrafish development. Electronic supplementary material The online version of this article (10.1186/s13059-019-1624-4) contains supplementary material, which is available to authorized users.

Published
2019

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