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Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology
- Source :
- Genome Biology, Vol 20, Iss 1, Pp 1-6 (2019), Genome Biology
- Publication Year :
- 2019
- Publisher :
- BMC, 2019.
-
Abstract
- Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embryos. Trim-Away depletes proteins faster than morpholinos, which enables analysis of protein function during early embryogenesis. Furthermore, Trim-Away can be applied to evaluate the role of maternally contributed proteins in zebrafish embryos. Our findings indicate that Trim-Away is a powerful tool to perform functional analysis of proteins during zebrafish development. Electronic supplementary material The online version of this article (10.1186/s13059-019-1624-4) contains supplementary material, which is available to authorized users.
- Subjects :
- Cell type
Embryo, Nonmammalian
animal structures
Morpholino
lcsh:QH426-470
ved/biology.organism_classification_rank.species
Short Report
Endogeny
Biology
Protein degradation
Animals, Genetically Modified
03 medical and health sciences
0302 clinical medicine
Animals
Model organism
Zebrafish
lcsh:QH301-705.5
030304 developmental biology
0303 health sciences
Gene knockdown
ved/biology
fungi
biology.organism_classification
Phenotype
Cell biology
lcsh:Genetics
Ribonucleoproteins
lcsh:Biology (General)
Trim-away
Proteolysis
embryonic structures
Knockdown
030217 neurology & neurosurgery
TRIM21
Biotechnology
Subjects
Details
- Language :
- English
- Volume :
- 20
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Genome Biology
- Accession number :
- edsair.doi.dedup.....b2e875ec1203809c45656322b9e362a5