1. Purification and characterization of an antioxidant protein from Ginkgo biloba seeds
- Author
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Huang, Wen, Deng, Qianchun, Xie, Bijun, Shi, John, Huang, FengHong, Tian, Binqiang, Huang, Qingde, and Xue, Sophia
- Subjects
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ANTIOXIDANTS , *CHEMICAL purification , *GINKGO , *CHEMILUMINESCENCE , *MOLECULAR weights , *POLYSACCHARIDES , *DRUG activation - Abstract
Abstract: A new antioxidation protein named G4b was purified from Ginkgo biloba seed albumin. The results of chemical colorimetry and chemiluminescence methods showed that the hemi-inhibitory concentration (IC50) of G4b on the hydroxyl radical were 78.1μg/mL and 115.1μg/mL, and the IC50 on DPPH was 100.7μg/mL, while the IC50 on the superoxide anion and DNA injury were 23μg/mL and 27μg/mL, respectively, and the abundance of sulfur amino acids and aromatic amino acids contained in G4b might contribute to its strong antioxidation properties. G4b is a kind of homogeneous and novel protein with a molecular mass of 29,247Da, consisting of two peptides with similar molecular weights linked by a disulfide bond. Result of β-elimination reaction showed that G4b is connected with a small quantity of polysaccharide by an oxygen-glucosidic bond. [Copyright &y& Elsevier]
- Published
- 2010
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